I received this message from Paula Flicker and offered to post it on the microscopy list server. If you are interested, if possible, answer directly to Paula. If you answer to me I will forward your message.
Michael Radermacher University of Vermont
X-from: "Flicker, Paula (NIH/NIGMS) [E]" {FLICKERP-at-NIGMS.NIH.GOV}
A non-scientist colleague has purchased a house with an electron microscope in the basement. She brought in the manual. It is an RCA EMU-4 owned by a George Christov.
Any ideas of someone who wants this machine and could come take it away? Otherwise, instruction on how to properly dispose of it would be welcome.
thank you, Paula
Paula F. Flicker, Ph.D. Program Director, NIGMS Building 45, Room 2AS.13H 45 Center Drive MSC 6200 Bethesda, MD 20892-6200 301-594-0828 fax: 301-480-2004
==============================Original Headers============================== 12, 27 -- From mraderma-at-physiology.med.uvm.edu Mon Oct 1 13:45:28 2007 12, 27 -- Received: from eagle.uvm.edu (eagle.uvm.edu [132.198.101.203]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l91IjSrt020354 12, 27 -- for {microscopy-at-microscopy.com} ; Mon, 1 Oct 2007 13:45:28 -0500 12, 27 -- Received: from physiology.med.uvm.edu (physiology.med.uvm.edu [132.198.52.4]) 12, 27 -- by eagle.uvm.edu (8.13.7/8.13.5) with ESMTP id l91IjRSb008598 12, 27 -- for {microscopy-at-microscopy.com} ; Mon, 1 Oct 2007 14:45:27 -0400 12, 27 -- Received: from physiology.med.uvm.edu (localhost [127.0.0.1]) 12, 27 -- by physiology.med.uvm.edu (Postfix) with ESMTP id B73D2F326F 12, 27 -- for {microscopy-at-microscopy.com} ; Mon, 1 Oct 2007 14:45:27 -0400 (EDT) 12, 27 -- Received: (from apache-at-localhost) 12, 27 -- by physiology.med.uvm.edu (8.12.8/8.12.8/Submit) id l91IjQTn017496 12, 27 -- for microscopy-at-microscopy.com; Mon, 1 Oct 2007 14:45:26 -0400 12, 27 -- From: Michael Radermacher {mraderma-at-physiology.med.uvm.edu} 12, 27 -- X-Authentication-Warning: physiology.med.uvm.edu: apache set sender to mraderma-at-physiology.med.uvm.edu using -f 12, 27 -- Received: from 132.198.90.90 ( [132.198.90.90]) 12, 27 -- as user mraderma-at-localhost by physiology.med.uvm.edu with HTTP; 12, 27 -- Mon, 1 Oct 2007 14:45:26 -0400 12, 27 -- Message-ID: {1191264326.47014046d1f8c-at-physiology.med.uvm.edu} 12, 27 -- Date: Mon, 1 Oct 2007 14:45:26 -0400 12, 27 -- To: microscopy-at-microscopy.com 12, 27 -- Subject: what to do with RCA EMU-4? 12, 27 -- MIME-Version: 1.0 12, 27 -- Content-Type: text/plain; charset=ISO-8859-1 12, 27 -- Content-Transfer-Encoding: 8bit 12, 27 -- User-Agent: Internet Messaging Program (IMP) 3.1 12, 27 -- X-Originating-IP: 132.198.90.90 ==============================End of - Headers==============================
Hello listservers, We have a Balzers Freeze etching system, BAF 400D, that is available for purchase. The system is operating, can do shadowing, etching and freeze fracture. It is an old system, so we are open to all offers.
Michael Delannoy Associate Director, JHMI Microscope Facility Dept. of Cell Biology Physiology G-04 725 N. Wolfe St. Baltimore, MD 21205 (410) 955-1365 office (410) 614-6890 lab
==============================Original Headers============================== 4, 27 -- From delannoy-at-jhmi.edu Mon Oct 1 13:49:33 2007 4, 27 -- Received: from ipex4.johnshopkins.edu (ipex4.johnshopkins.edu [128.220.161.141]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l91InXJM025023 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 1 Oct 2007 13:49:33 -0500 4, 27 -- X-IronPort-AV: E=Sophos;i="4.21,217,1188792000"; 4, 27 -- d="scan'208";a="30769812" 4, 27 -- Received: from jhem1.johnshopkins.edu ([10.181.31.201]) 4, 27 -- by ipex4.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 01 Oct 2007 14:49:03 -0400 4, 27 -- Received: from johnshopkins.edu ([10.181.31.209]) by jesmail.johnshopkins.edu 4, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 4, 27 -- with ESMTP id {0JP800DOVXLSFZ90-at-jesmail.johnshopkins.edu} for 4, 27 -- microscopy-at-microscopy.com; Mon, 01 Oct 2007 14:49:04 -0400 (EDT) 4, 27 -- Received: from [10.181.192.192] (Forwarded-For: [162.129.37.164]) 4, 27 -- by jesmail.johnshopkins.edu (mshttpd); Mon, 01 Oct 2007 14:49:04 -0400 4, 27 -- Date: Mon, 01 Oct 2007 14:49:04 -0400 4, 27 -- From: MICHAEL J DELANNOY {delannoy-at-jhmi.edu} 4, 27 -- Subject: Balzers freeze etch 4, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 4, 27 -- Message-id: {f53ddbd8ee3.470108e0-at-johnshopkins.edu} 4, 27 -- MIME-version: 1.0 4, 27 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built May 24 2006) 4, 27 -- Content-type: text/plain; charset=us-ascii 4, 27 -- Content-language: en 4, 27 -- Content-transfer-encoding: 7BIT 4, 27 -- Content-disposition: inline 4, 27 -- X-Accept-Language: en 4, 27 -- Priority: normal ==============================End of - Headers==============================
Hello listservers, We have a Balzers Freeze etching system, BAF 400D, that is available for purchase. The system is operating, can do shadowing, etching and freeze fracture. It is an old system, so we are open to all offers.
Michael Delannoy Associate Director, JHMI Microscope Facility Dept. of Cell Biology Physiology G-04 725 N. Wolfe St. Baltimore, MD 21205 (410) 955-1365 office (410) 614-6890 lab
==============================Original Headers============================== 4, 27 -- From delannoy-at-jhmi.edu Mon Oct 1 13:49:41 2007 4, 27 -- Received: from ipex1.johnshopkins.edu (ipex1.johnshopkins.edu [162.129.8.141]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l91InftS025196 4, 27 -- for {microscopy-at-msa.microscopy.com} ; Mon, 1 Oct 2007 13:49:41 -0500 4, 27 -- X-IronPort-AV: E=Sophos;i="4.21,217,1188792000"; 4, 27 -- d="scan'208";a="30941177" 4, 27 -- Received: from unknown (HELO jhem1.johnshopkins.edu) ([10.181.31.209]) 4, 27 -- by ipex1.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 01 Oct 2007 14:49:33 -0400 4, 27 -- Received: from johnshopkins.edu ([10.181.31.209]) by jesmail.johnshopkins.edu 4, 27 -- (Sun Java System Messaging Server 6.2-7.05 (built Sep 5 2006)) 4, 27 -- with ESMTP id {0JP800DSDXMMFZ90-at-jesmail.johnshopkins.edu} for 4, 27 -- microscopy-at-msa.microscopy.com; Mon, 01 Oct 2007 14:49:34 -0400 (EDT) 4, 27 -- Received: from [10.181.192.192] (Forwarded-For: [162.129.37.164]) 4, 27 -- by jesmail.johnshopkins.edu (mshttpd); Mon, 01 Oct 2007 14:49:34 -0400 4, 27 -- Date: Mon, 01 Oct 2007 14:49:34 -0400 4, 27 -- From: MICHAEL J DELANNOY {delannoy-at-jhmi.edu} 4, 27 -- Subject: Balzers 4, 27 -- To: "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} 4, 27 -- Message-id: {f55ac50f15a9.470108fe-at-johnshopkins.edu} 4, 27 -- MIME-version: 1.0 4, 27 -- X-Mailer: Sun Java(tm) System Messenger Express 6.2-5.03 (built May 24 2006) 4, 27 -- Content-type: text/plain; charset=us-ascii 4, 27 -- Content-language: en 4, 27 -- Content-transfer-encoding: 7BIT 4, 27 -- Content-disposition: inline 4, 27 -- X-Accept-Language: en 4, 27 -- Priority: normal ==============================End of - Headers==============================
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==============================Original Headers============================== 8, 22 -- From donovan-at-uoregon.edu Mon Oct 1 14:00:27 2007 8, 22 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu [128.223.142.40]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l91J0Rfh023788 8, 22 -- for {microscopy-at-microscopy.com} ; Mon, 1 Oct 2007 14:00:27 -0500 8, 22 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.92.75]) 8, 22 -- (authenticated bits=0) 8, 22 -- by smtp.uoregon.edu (8.13.8/8.13.8) with ESMTP id l91J0QxJ032115 8, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 8, 22 -- Mon, 1 Oct 2007 12:00:26 -0700 8, 22 -- Message-Id: {200710011900.l91J0QxJ032115-at-smtp.uoregon.edu} 8, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 22 -- Date: Mon, 01 Oct 2007 11:59:17 -0700 8, 22 -- To: microscopy-at-microscopy.com 8, 22 -- From: John Donovan {donovan-at-uoregon.edu} 8, 22 -- Subject: Re: [Microscopy] what to do with RCA EMU-4? 8, 22 -- Cc: mraderma-at-physiology.med.uvm.edu 8, 22 -- In-Reply-To: {200710011853.l91IrOvt005623-at-ns.microscopy.com} 8, 22 -- References: {200710011853.l91IrOvt005623-at-ns.microscopy.com} 8, 22 -- Mime-Version: 1.0 8, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 22 -- X-Virus-Scanned: ClamAV 0.91.2/4445/Mon Oct 1 01:32:46 2007 on mserv1 8, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Well, I can't help with the scope as a whole, but I can say that the Wehnelt chambers make great odds and ends holders for your desk. That nifty RCA logo in gold-colored metal will make you the envy of your coworkers. You can use them for planters, too!
Randy
-----Original Message----- X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu] Sent: Monday, October 01, 2007 2:02 PM To: Tindall, Randy D.
In case anyone is interested here is a picture of it!
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==============================Original Headers============================== 8, 22 -- From donovan-at-uoregon.edu Mon Oct 1 14:00:27 2007 8, 22 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu [128.223.142.40]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l91J0Rfh023788 8, 22 -- for {microscopy-at-microscopy.com} ; Mon, 1 Oct 2007 14:00:27 -0500 8, 22 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.92.75]) 8, 22 -- (authenticated bits=0) 8, 22 -- by smtp.uoregon.edu (8.13.8/8.13.8) with ESMTP id l91J0QxJ032115 8, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 8, 22 -- Mon, 1 Oct 2007 12:00:26 -0700 8, 22 -- Message-Id: {200710011900.l91J0QxJ032115-at-smtp.uoregon.edu} 8, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 22 --
In addition to the G1 epoxy compound sold by Gatan, Varian markets a UHV epoxy called Torr Seal that is rated for pressures down to 10-9 Torr. The temperature range quoted for this material is -45 to +120°C, +120°C being the common bake-out temperature for vacuum systems. However, this is a pretty hard and dense material when fully cured, and might possibly serve at considerably higher temperatures. I don't have any information on this matter. LDS Vacuum sells an Epoxy that is advertised as being equivalent to Torr Seal, and which is now on sale at a very reasonable price. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 2, 16 -- From bigelow-at-umich.edu Mon Oct 1 14:44:55 2007 2, 16 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81]) 2, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l91Jitq3016237 2, 16 -- for {microscopy-at-microscopy.com} ; Mon, 1 Oct 2007 14:44:55 -0500 2, 16 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 2, 16 -- BY hackers.mr.itd.umich.edu ID 47014E35.EE4A4.31411 ; 2, 16 -- 1 Oct 2007 15:44:54 -0400 2, 16 -- Mime-Version: 1.0 2, 16 -- Message-Id: {p06240800c326fccd5069-at-[141.212.131.221]} 2, 16 -- Date: Mon, 1 Oct 2007 15:44:52 -0400 2, 16 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 2, 16 -- From: Wil Bigelow {bigelow-at-umich.edu} 2, 16 -- Subject: [Microscopy] RE: UHV Epoxy 2, 16 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 2, 16 -- Content-Transfer-Encoding: 8bit 2, 16 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l91Jitq3016237 ==============================End of - Headers==============================
There is an article by Fiore and Herring in the first MRS TEM prep book on a ceramic high temperature epoxy solution for hot stages. MRS Vol 115, page 125 that describes a cross section technique by putting their samples in a cast block of ceramic adhesive. The ceramic adhesive that they used was Ceramabond 569 from Aremco Products, Inc. They were able to use it up to approximately 1300C.
I thought that I had a paper that showed the same ceramic adhesive used as a glue joint between two pieces, but I can't find it.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: zhangj16-at-msu.edu [mailto:zhangj16-at-msu.edu] Sent: Sunday, September 30, 2007 12:16 PM To: Walck-at-SouthBayTech.com
Dear all
I have the question about how high temperature Gatan G1 epoxy can survive for a in-situ TEM study. I dont have the instruction of G1 in hand right now. Thank you for any opinion and experience.
Jiaming Zhang
Research Assistant Department of Chem. Eng. & Mater. Sci. Michigan State University Tel: 517-231-8013 Email: zhangj16-at-egr.msu.edu
==============================Original Headers============================== 6, 16 -- From zhangj16-at-msu.edu Sun Sep 30 14:09:25 2007 6, 16 -- Received: from sys25.mail.msu.edu (sys25.mail.msu.edu [35.9.75.125]) 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8UJ9Pq2015014 6, 16 -- for {microscopy-at-microscopy.com} ; Sun, 30 Sep 2007 14:09:25 -0500 6, 16 -- Received: from zhangj16 by sys25.mail.msu.edu with local (Exim 4.63 #1) 6, 16 -- id 1Ic4AP-00033Q-12 6, 16 -- for microscopy-at-microscopy.com; Sun, 30 Sep 2007 15:09:25 -0400 6, 16 -- From: "Jiaming Zhang" {zhangj16-at-msu.edu} 6, 16 -- To: microscopy-at-microscopy.com 6, 16 -- Subject: Ask about how high temperature Gatan G1 epoxy can survive 6, 16 -- Date: Sun, 30 Sep 2007 15:09:24 -0400 6, 16 -- Mime-Version: 1.0 6, 16 -- Content-Type: text/plain; format=flowed; charset="utf-8" 6, 16 -- Content-Transfer-Encoding: 7bit 6, 16 -- Message-Id: {E1Ic4AP-00033Q-12-at-sys25.mail.msu.edu} 6, 16 -- X-Virus: None found by Clam AV ==============================End of - Headers==============================
==============================Original Headers============================== 19, 22 -- From walck-at-southbaytech.com Mon Oct 1 22:42:43 2007 19, 22 -- Received: from flpi185.prodigy.net (flpi185.sbcis.sbc.com [207.115.20.187]) 19, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l923ggPn010606 19, 22 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Oct 2007 22:42:42 -0500 19, 22 -- X-ORBL: [64.169.217.123] 19, 22 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 19, 22 -- by flpi185.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id l923gtY7000950; 19, 22 -- Mon, 1 Oct 2007 20:42:56 -0700 19, 22 -- From: "Scott Walck" {walck-at-southbaytech.com} 19, 22 -- To: {Microscopy-at-microscopy.com} 19, 22 -- Cc: {zhangj16-at-msu.edu} 19, 22 -- Subject: High Temp epoxy 19, 22 -- Date: Mon, 1 Oct 2007 20:43:01 -0700 19, 22 -- Message-ID: {005001c804a6$55ab21a0$7801a8c0-at-dynamicbl8uno3} 19, 22 -- MIME-Version: 1.0 19, 22 -- Content-Type: text/plain; 19, 22 -- charset="us-ascii" 19, 22 -- Content-Transfer-Encoding: 7bit 19, 22 -- X-Mailer: Microsoft Office Outlook 11 19, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 19, 22 -- Thread-Index: AcgDlklP0cry0khdSDq36vZa1Yms4QBDI8Kg 19, 22 -- In-Reply-To: {200709301915.l8UJFcPQ020500-at-ns.microscopy.com} ==============================End of - Headers==============================
RESEARCH TECHNICIAN POSITION IN MEMBRANE PROTEIN CRYSTALLIZATION.
A research technician position is available immediately at the New York Structural Biology Center in New York City (http://www.nysbc.org/facilities/CEM). The candidate will focus on implementing a high-throughput screen to identify conditions for crystallizing membrane proteins for analysis by cryo-electron microscopy. This work will involve setting up parallel crystallization trials using a liquid-handling robot and a microdialysis block designed in our laboratory. The plan is to image these samples using automated software and a 100-sample multi-specimen holder to be purchased from a commercial source and implemented at NYSBC. The candidates will be part of an energetic NIH-funded research center focused on membrane protein structure determination by X-ray crystallography and cryo-electron crystallography.
Our laboratory is fully equipped for all aspects of protein expression, crystallization and electron microscopy. We have a strong collaboration with a group involved in protein target selection, cloning and expression screening as part of the NIH Protein Structure Initiative. The current position requires skills in electron microscopy. Although specific experience is preferred, general experience in imaging and/or biophysics may suffice.
The initial appointment is for one year and renewable for up to three years. Interested candidates should e-mail cover letter, full CV and contact information for three professional referees to:
David Stokes stokes-at-nysbc.org New York Structural Biology Center tel: 212-939-0660 x9116 http://nysbc.org/ fax: 646-219-0300
==============================Original Headers============================== 8, 22 -- From kderr-at-nysbc.org Tue Oct 2 11:01:25 2007 8, 22 -- Received: from nysbc.org (nysbc.org [209.83.182.194]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l92G1PFM011598 8, 22 -- for {Microscopy-at-Microscopy.Com} ; Tue, 2 Oct 2007 11:01:25 -0500 8, 22 -- Received: from CRYOEM03 ([10.10.10.1]) 8, 22 -- by nysbc.org (Kerio MailServer 6.3.1) 8, 22 -- for Microscopy-at-Microscopy.Com; 8, 22 -- Tue, 2 Oct 2007 12:05:38 -0400 8, 22 -- From: "kd Derr" {kderr-at-nysbc.org} 8, 22 -- To: {Microscopy-at-Microscopy.Com} 8, 22 -- Subject: Tech position in membrane protein crystallization 8, 22 -- Date: Tue, 2 Oct 2007 11:52:49 -0400 8, 22 -- Message-ID: {000901c8050c$48b30f60$7f05a8c0-at-CRYOEM03} 8, 22 -- MIME-Version: 1.0 8, 22 -- Content-Type: text/plain; 8, 22 -- charset="US-ASCII" 8, 22 -- Content-Transfer-Encoding: 7bit 8, 22 -- X-Priority: 3 (Normal) 8, 22 -- X-MSMail-Priority: Normal 8, 22 -- X-Mailer: Microsoft Outlook, Build 10.0.2627 8, 22 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2800.1896 8, 22 -- Importance: Normal ==============================End of - Headers==============================
Yesterday I was cleaning the condenser pole piece of this sem, and acidentally messed up the two small pole pieces on the table. There is no damage done in the pieces, but I do not know now how to assemble. The question is: there are two pole pairs, with different hole diameters? So I have to pair them according to the hole diameter, or not?
Thanks in advance,
Jakab-Farkas Laszlo Laboratory technician
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This Question was submitted to Ask-A-Microscopist by (jhe1-at-tulane.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, October 2, 2007 at 10:58:31 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both jhe1-at-tulane.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: jhe1-at-tulane.edu Name: Jibao He
Organization: Tulane University
Education: Graduate College
Title: Image analysis software
Question: Dear Colleagues:
We are looking for image analysis software to process TEM and SEM images to get size distribution information. If you are using a good one, please recommend it to us including purchase contact information.
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Email: opmills-at-mtu.edu Name: Owen Mills
Organization: Michigan Tech University
Title-Subject: [Filtered] textured substrate
Question: All,
I need a substrate with a fairly uniform .5 to 1um texture. I'll be depositing ~1 um and larger particles on the substrate for 3D SEM study. The sample will be carbon coated. The substrate should be something that is cheap, can be cut to put on a SEM mount and easy to acquire.
Do you think etching a substrate of some kind would produce the desired texture? Any ideas what would be suitable for this etching?
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Title-Subject: [Filtered] Windows on the NanoWorld - FREE Workshop
Question: A FREE workshop and hands-on forum on electron and ion beam imaging and analysis with speakers from Industry, Academia and Instrument Design.
October 23-25, 2007 Clemson University/Advanced Materials Research Lab Clemson, SC
Tuesday, October 23, 2007 (at the Clemson Conference Center) Introduction to Advanced Analytical and Imaging Techniques
Wednesday, October 24, 2007 (at the Clemson AMRL) Advanced Imaging and Analysis: low kV (SEM, STEM, FIB + SEM...)
Thursday, October 25, 2007 (at the Clemson AMRL)Advanced Imaging and Analysis: high kV (TEM, STEM...)
Presentations will be given by expert speakers in a casual seminar format and will be complemented by lab based investigations of advanced topics. Technique intro-ductions will be given on the first day, advanced case-studies and research based topics on the second and third day. There will also be opportunities to run your own samples on advanced imaging and analysis tools in the Clemson Advanced Materials Research Lab.
For questions and hotel reservations please e-mail NSDevent-at-hitachi-hta.com or contact Beth Moseley beth.moseley-at-hitachi-hta.com) at 925.218.2821. Please RSVP by Monday, October 15, 2007 to attend.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both klangwor-at-uoregon.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: klangwor-at-uoregon.edu Name: Kurt Langworthy
Organization: University of Oregon
Title-Subject: [Filtered] SEM Nano-particle contamination
Question: I've noticed the gun arcing on our Zeiss FEG SEM (the arcing can be heard easily and seen on our ampmeter even with the beam blanked!). Due to the amount of nano-particle and nano-wire samples we've recently been working with I've become curious if nano-particle contamination is a possible contributor to the poor performance of the gun. Have any of you experienced nano-particle contamination in your systems? What sort of behaviors did you document? Best Regards, Kurt
Kurt Langworthy CAMCOR University of Oregon 541-346-4778
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at {http://www.microscopy.org/MicroscopyListserver/MLFormMail.htmlhttp://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both {mailto:amgusman-at-ucdavis.eduamgusman-at-ucdavis.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: {mailto:amgusman-at-ucdavis.eduamgusman-at-ucdavis.edu Name: Andrea Gusman
Question: I am trying to determine how thick my sputter coater will coat at different time intervals so I am conducting an experiment. I made a small block out of Spurrs resin, sputter coated it, and then placed the block back in the mold and covered the gold layer with more Spurrs to sandwich it between resin. I then trimmed the block and cross sectioned it on a microtome to obtain thin sections. I placed the thin sections in the TEM and measured the gold layer. The time intervals I used were 30 seconds, 90 seconds, 1 min, and 2 min. Does anyone know how thick of a layer a sputter coater should coat with a gold target at those time intervals? Or what is a suitable thickness for SEM samples? Also does anyone have any other suggestions on ways to determine the thickness besides thin sectioning? Thanks!
The way you are doing this is exactly what one should do for a check of the system in the conditions you use. The TEM measurement of the layer will be an accurate value to use. For given conditions this should be very repeatable. The thickness of the layer is dependent on many things including the design of the coater/electrodes. For a given unit the coating thickness will be directly proportional to the CURRENT and TIME; the current is affected by the kV applied, the gas (typically Argon), and the pressure of the gas.
For the Polaron E5100 sputter coater the following formula applies: T = mA * kV * t * k
T=thickness (Angstroms = 1x10^-10 meters) kV = 2.2 mA is the set/observed current k (Argon) = 5 Note: k (Nitrogen) = 2 (k is a factor based on the gas and the system design) For the E5100....... } Time (min) } mA 1 2 3 } 5 55 110 165 } 6 66 132 198 } 7 77 154 231 } 8 88 176 264 } 9 99 198 297 } 10 110 220 330 } 11 121 242 363 } 12 132 264 396 } 13 143 286 429 } 14 154 308 462 } 15 165 330 495 } 16 176 352 528 } 17 187 374 561 } 18 198 396 594 } 19 209 418 627 } 20 220 440 660
If you have a manual for the sputter coater it may give a formula that accounts for the design of the system and lets you plug in the parameters you can control (voltage and current, and time). Contact the manufacturer if you don't have a manual, or else get back to the list with the make/model of the machine and the parameters you are using and someone will probably be able to help.
Note that sputtered layers much below 10nm are likely to be discontinuous and in cross section it may appear as a discontinuous band.
Hope this helps.
Dale
amgusman-at-ucdavis.edu wrote: -------------------------------------------------------------------- } } Email: {mailto:amgusman-at-ucdavis.eduamgusman-at-ucdavis.edu } Name: Andrea Gusman } } Title-Subject: [Filtered] Sputter coater thickness } } Question: I am trying to determine how thick my sputter coater will coat at different time intervals so I am conducting an experiment. I made a small block out of Spurrs resin, sputter coated it, and then placed the block back in the mold and covered the gold layer with more Spurrs to sandwich it between resin. I then trimmed the block and cross sectioned it on a microtome to obtain thin sections. I placed the thin sections in the TEM and measured the gold layer. } The time intervals I used were 30 seconds, 90 seconds, 1 min, and 2 min. } Does anyone know how thick of a layer a sputter coater should coat with a gold target at those time intervals? Or what is a suitable thickness for SEM samples? } Also does anyone have any other suggestions on ways to determine the thickness besides thin sectioning? } Thanks! } } } --------------------------------------------------------------------------- } } } -- } Andrea Gusman } Staff Research Associate I } Chemical Engineering & Material Science } University of California, Davis } 530-752-0284 } } ==============================Original Headers============================== } 4, 11 -- From zaluzec-at-microscopy.com Tue Oct 2 20:50:30 2007 } 4, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 4, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l931oSSS005332 } 4, 11 -- for {microscopy-at-microscopy.com} ; Tue, 2 Oct 2007 20:50:29 -0500 } 4, 11 -- Mime-Version: 1.0 } 4, 11 -- Message-Id: {p06240809c328a4e12a4e-at-[206.69.208.22]} } 4, 11 -- Date: Tue, 2 Oct 2007 20:50:27 -0500 } 4, 11 -- To: microscopy-at-microscopy.com } 4, 11 -- From: Andrea Gusman {amgusman-at-ucdavis.edu} (by way of MicroscopyListserver) } 4, 11 -- Subject: MicroscopyListserverviaWWW: Sputter coater thickness } 4, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
-- } {(((º} L L } {(((º} .·´¯`·.¸ {º)))} { ¸.·´¯`·.¸ } {(((º} ¸.·´¯¯¯¯¯¯¯
Dale A. Callaham Central Microscopy Facility The University of Massachusetts Amherst, MA 01003
==============================Original Headers============================== 11, 20 -- From dac-at-research.umass.edu Tue Oct 2 22:18:54 2007 11, 20 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l933Isct019365 11, 20 -- for {microscopy-at-microscopy.com} ; Tue, 2 Oct 2007 22:18:54 -0500 11, 20 -- Received: from [192.168.1.3] (68-112-235-86.dhcp.oxfr.ma.charter.com [68.112.235.86]) 11, 20 -- (authenticated bits=0) 11, 20 -- by race5.oit.umass.edu (8.14.1/8.14.1) with ESMTP id l933IokP004107 11, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 11, 20 -- Tue, 2 Oct 2007 23:18:51 -0400 11, 20 -- Message-ID: {4703189B.4030802-at-research.umass.edu} 11, 20 -- Date: Tue, 02 Oct 2007 23:20:43 -0500 11, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 11, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.6) Gecko/20070802 SeaMonkey/1.1.4 11, 20 -- MIME-Version: 1.0 11, 20 -- To: amgusman-at-ucdavis.edu, Microscopy List {microscopy-at-microscopy.com} 11, 20 -- Subject: Re: [Microscopy] Sputter coater thickness 11, 20 -- References: {200710030155.l931tfMv014430-at-ns.microscopy.com} 11, 20 -- In-Reply-To: {200710030155.l931tfMv014430-at-ns.microscopy.com} 11, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
a similar question came up in July about measuring sputter coating thicknes and I suggested the following as a simple alternative:
a simple method that I used to check the thickness of sputter coatings was to photograph some spherical particles on carbon/formvar coated grids in the TEM (I used both 0.142um latex beads and 20nm colloidal gold). The trick is to find the same particles before and after coating so I simply photographed a distinctive cluster very near the centre of the grid. I then sputtered the grids in the coater and re-photographed. In my case I halved the diameter difference for a few particles for thickness.
....
This may not be the perfect method but it's quick and easy if you have the bits and of course the TEM. A refinement would be to use coated finder grids but I simply used some centre marked grids and it only took a few minutes to find the same particles after coating. The only other problem would be if the coatings get greater than 20-30nm the grid background may become denser.
If you're keen you could compare both methods, but then how would you know which was most accurate. I hope this helps.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK
e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: dac-at-research.umass.edu
Owen,
Do you have other requirements for the substrate? Conductivity, etc.
Try this: make a monolayer of beads, silica, polystyrene, whatever. Carefully layer over that something like methyl methacrylate. Polymerize and dissove the beads (HF for silica, etc.). This will leave the plastic with a texture the size of the beads, although as indentations. If you need positive relief (domes), the methacrylate can be used as a mold to make a second cast. This is a simplified version of the procedure Paul Sims and Ralph Albrecht used to make casts of collagen films with 20 nm resolution. I've got the paper in a box somewhere, but you could email Ralph (UW-Madison) for a copy.
If the final surface needs to be conductive, you can sputter-coat it.
Phil
} Email: opmills-at-mtu.edu } Name: Owen Mills } } Organization: Michigan Tech University } } Title-Subject: [Filtered] textured substrate } } Question: All, } } I need a substrate with a fairly uniform .5 to 1um texture. I'll be } depositing ~1 um and larger particles on the substrate for 3D SEM } study. The sample will be carbon coated. The substrate should be } something that is cheap, can be cut to put on a SEM mount and easy } to acquire. } } Do you think etching a substrate of some kind would produce the } desired texture? Any ideas what would be suitable for this etching? } } Thanks, } } Owen Mills } Michigan Tech } } Login Host: 141.219.192.45 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859
==============================Original Headers============================== 6, 23 -- From oshel1pe-at-cmich.edu Wed Oct 3 07:29:16 2007 6, 23 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l93CTGIT030301 6, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Oct 2007 07:29:16 -0500 6, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 6, 23 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id l93Cnw9N018469; 6, 23 -- Wed, 3 Oct 2007 08:49:58 -0400 6, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 6, 23 -- Wed, 3 Oct 2007 08:29:10 -0400 6, 23 -- Mime-Version: 1.0 6, 23 -- Message-Id: {f06240804c32939263a3c-at-[141.209.160.249]} 6, 23 -- In-Reply-To: {200710030106.l9316kXV017447-at-ns.microscopy.com} 6, 23 -- References: {200710030106.l9316kXV017447-at-ns.microscopy.com} 6, 23 -- Date: Wed, 3 Oct 2007 08:29:11 -0400 6, 23 -- To: opmills-at-mtu.edu 6, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 23 -- Subject: Re: [Microscopy] viaWWW: textured substrate 6, 23 -- Cc: Microscopy-at-microscopy.com 6, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 6, 23 -- X-OriginalArrivalTime: 03 Oct 2007 12:29:11.0326 (UTC) FILETIME=[005D43E0:01C805B9] 6, 23 -- X-CanItPRO-Stream: default 6, 23 -- X-Spam-Score: -4 () L_EXCH_MF 6, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
Ballpark, the thickness is roughly 10nm/minute in our Denton Desk II with gold or gold/palladium. But, this is strongly dependent on the gas pressure in the chamber, current, target (Au, Au/Pd, or something else) and target-sample distance, so that figure may not be relevant to your coater.
The second question, how thick for samples, is entirely dependent on 1) what kind of samples (how conductive are they, what's the surface topography [smooth, setose, etc.], 2) what accelerating voltage do you need (low voltage, less coating maybe), 3) how are the samples mounted? better the electrical mounts, therefore path to ground, can allow for thinner coats 4) what resolution (what magnification) do you need -- the higher the mag/resolution the thinner the coat required (thick of looking at a lawn after a the first snowfall. If the snow is light, it coats all the grass blades, but you can still see each individual blade and it's veins; if the snow is heavy, all you see is the snow. 5) Etc. So there's no simple answer to "how much?" It depends on "what questions?"
Phil
} This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } {http://www.microscopy.org/MicroscopyListserver/MLFormMail.htmlhttp://www.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both {mailto:amgusman-at-ucdavis.eduamgusman-at-ucdavis.edu } as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: {mailto:amgusman-at-ucdavis.eduamgusman-at-ucdavis.edu } Name: Andrea Gusman } } Title-Subject: [Filtered] Sputter coater thickness } } Question: I am trying to determine how thick my sputter coater will } coat at different time intervals so I am conducting an experiment. I } made a small block out of Spurrs resin, sputter coated it, and then } placed the block back in the mold and covered the gold layer with } more Spurrs to sandwich it between resin. I then trimmed the block } and cross sectioned it on a microtome to obtain thin sections. I } placed the thin sections in the TEM and measured the gold layer. } The time intervals I used were 30 seconds, 90 seconds, 1 min, and 2 min. } Does anyone know how thick of a layer a sputter coater should coat } with a gold target at those time intervals? Or what is a suitable } thickness for SEM samples? } Also does anyone have any other suggestions on ways to determine } the thickness besides thin sectioning? } Thanks! } } } --------------------------------------------------------------------------- } } } -- } Andrea Gusman } Staff Research Associate I } Chemical Engineering & Material Science } University of California, Davis } 530-752-0284 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859
==============================Original Headers============================== 5, 24 -- From oshel1pe-at-cmich.edu Wed Oct 3 07:45:44 2007 5, 24 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l93CjiHS010170 5, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Oct 2007 07:45:44 -0500 5, 24 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 24 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id l93D6B9b020590; 5, 24 -- Wed, 3 Oct 2007 09:06:25 -0400 5, 24 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 5, 24 -- Wed, 3 Oct 2007 08:45:34 -0400 5, 24 -- Mime-Version: 1.0 5, 24 -- Message-Id: {f06240805c3293bc8d850-at-[141.209.160.249]} 5, 24 -- In-Reply-To: {200710030154.l931sfkZ011872-at-ns.microscopy.com} 5, 24 -- References: {200710030154.l931sfkZ011872-at-ns.microscopy.com} 5, 24 -- Date: Wed, 3 Oct 2007 08:45:35 -0400 5, 24 -- To: amgusman-at-ucdavis.edu 5, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 24 -- Subject: Re: [Microscopy] MicroscopyListserverviaWWW: Sputter coater 5, 24 -- thickness 5, 24 -- Cc: Microscopy-at-microscopy.com 5, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 24 -- X-OriginalArrivalTime: 03 Oct 2007 12:45:34.0857 (UTC) FILETIME=[4A982F90:01C805BB] 5, 24 -- X-CanItPRO-Stream: default 5, 24 -- X-Spam-Score: -4 () L_EXCH_MF 5, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
A fast way to evaluate the layer thickness is to put the sample on a OM glass slide, and coat both together. After coating, one look at the color of the layer on the slide, by transparency in front of a sheet of paper (at daylight). Blue is in the 5-10 nm range, then green is up to 20 nm, green/yelowish is 20-30 nm, yellow more then 30 nm, and the transparency desappears (against the daylight) with more than 100 nm. In the same time, on can control the size and homogeneity of the deposit, versus current, distance to the target, and gas pressure.
With the way you have used combined with this one, you can calibrate the slides.
The needed thickness is depending of much things : what kind of SEM you have (W source or FEG), what kind of sample you are looking, what are the performences/quality of the coater (ultimate vaccum, quality of the magnetron head, is the power supply in current or voltage regulated, etc.).
The "thickness" is a bit a miss-leading concept, in that matter. Most sputter coaters give on most samples gold grains, and not a layer (sugar powdered on the cake and not butter spread on the bread). What one need is the smallest grains, separated by a distance short enough to allow the electron to tunnel from one to the next. And in most case, one have big grains, separated by a too long distance. On must than let the grains grow in size (and thickness) until there is a contact between them. And then, the film is too thick, like a ft. snow on a ploughed field, instead of only a layer of frost... If you need to look at a tree in the field, it doesn't matter, but if you are searching the seeds between the groves, you must try something other...
I would say : put the less gold necessary to have the picture you need, and try in the same time the lowest beam energy which gives a resolution adequate with the details you want to see.
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
amgusman-at-ucdavis.edu a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at {http://www.microscopy.org/MicroscopyListserver/MLFormMail.htmlhttp://www.microscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both {mailto:amgusman-at-ucdavis.eduamgusman-at-ucdavis.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: {mailto:amgusman-at-ucdavis.eduamgusman-at-ucdavis.edu } Name: Andrea Gusman } } Title-Subject: [Filtered] Sputter coater thickness } } Question: I am trying to determine how thick my sputter coater will coat at different time intervals so I am conducting an experiment. I made a small block out of Spurrs resin, sputter coated it, and then placed the block back in the mold and covered the gold layer with more Spurrs to sandwich it between resin. I then trimmed the block and cross sectioned it on a microtome to obtain thin sections. I placed the thin sections in the TEM and measured the gold layer. } The time intervals I used were 30 seconds, 90 seconds, 1 min, and 2 min. } Does anyone know how thick of a layer a sputter coater should coat with a gold target at those time intervals? Or what is a suitable thickness for SEM samples? } Also does anyone have any other suggestions on ways to determine the thickness besides thin sectioning? } Thanks! } } } --------------------------------------------------------------------------- } } } -- } Andrea Gusman } Staff Research Associate I } Chemical Engineering & Material Science } University of California, Davis } 530-752-0284 } } ==============================Original Headers============================== } 4, 11 -- From zaluzec-at-microscopy.com Tue Oct 2 20:50:30 2007 } 4, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 4, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l931oSSS005332 } 4, 11 -- for {microscopy-at-microscopy.com} ; Tue, 2 Oct 2007 20:50:29 -0500 } 4, 11 -- Mime-Version: 1.0 } 4, 11 -- Message-Id: {p06240809c328a4e12a4e-at-[206.69.208.22]} } 4, 11 -- Date: Tue, 2 Oct 2007 20:50:27 -0500 } 4, 11 -- To: microscopy-at-microscopy.com } 4, 11 -- From: Andrea Gusman {amgusman-at-ucdavis.edu} (by way of MicroscopyListserver) } 4, 11 -- Subject: MicroscopyListserverviaWWW: Sputter coater thickness } 4, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 14, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Oct 3 08:00:19 2007 14, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.153]) 14, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l93D0IJm022367 14, 29 -- for {microscopy-at-microscopy.com} ; Wed, 3 Oct 2007 08:00:19 -0500 14, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 14, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id l93D0FNn059648 14, 29 -- for {microscopy-at-microscopy.com} ; Wed, 3 Oct 2007 15:00:15 +0200 (CEST) 14, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 14, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 8592A106E6B0 14, 29 -- for {Microscopy-at-Microscopy.Com} ; Wed, 3 Oct 2007 14:59:39 +0200 (CEST) 14, 29 -- Message-ID: {47039249.3020005-at-ipcms.u-strasbg.fr} 14, 29 -- Date: Wed, 03 Oct 2007 14:59:53 +0200 14, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 14, 29 -- User-Agent: Thunderbird 1.5.0.13 (X11/20070824) 14, 29 -- MIME-Version: 1.0 14, 29 -- To: Microscopy-at-microscopy.com 14, 29 -- Subject: Re: [Microscopy] MicroscopyListserverviaWWW: Sputter coater thickness 14, 29 -- References: {200710030155.l931tVJ2013948-at-ns.microscopy.com} 14, 29 -- In-Reply-To: {200710030155.l931tVJ2013948-at-ns.microscopy.com} 14, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 29 -- Content-Transfer-Encoding: 8bit 14, 29 -- X-IPCMS-MailScanner: Found to be clean 14, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 14, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.153]); Wed, 03 Oct 2007 15:00:15 +0200 (CEST) 14, 29 -- X-Virus-Scanned: ClamAV 0.88.7/4461/Wed Oct 3 10:50:48 2007 on mr3.u-strasbg.fr 14, 29 -- X-Virus-Status: Clean 14, 29 -- X-Spam-Status: No, score=-0.3 required=5.0 tests=AWL autolearn=disabled 14, 29 -- version=3.1.8 14, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr3.u-strasbg.fr ==============================End of - Headers==============================
I didn't see much answer to your question. So here are my 2 cents.
If the first generations of the scroll pumps were not very reliable, with mechanical problems and generating much dust, what the manufacturers can offer now is in most case correct.
The first limitation of these pumps, is the ultimate vacuum. Two stages pumps go down to 4E-2 mbar, three stages pumps to less than E-2 mbar. This means that one can use a scroll as roughing pump neither for a diff pump, nor for a old turbo pump, which hasn't Gaede or Holweck stages at the high vac side. With such turbos, one could perhaps work in a stationary mode (high vac, virtually no flow), put it will stall as soon as one have a consequent flow (starting up from the atmospheric pressure, process with a gas inlet).
Scroll pumps are made to work with newer turbo pumps (so-called macro-torr, turbo-drag, compound, molecular, hybrid, etc), which support a few mbar at their roughing side, typically the vacuum of a diaphragm pump. In comparison with the diaphragm pump, the use of a scroll gives a better ultimate vacuum, less noise, a bit less maintenance, a much more flow.
In comparison with the rotary vane pump, one can give some pro and contra for each.
Pro Scroll :
Clean, "dry" (i.e. without hydrocarbons) vacuum. Seal exchange is very easy and fast
Contra :
Expensive Anualy seal exchange (c.f. above) Seal is expensive too (but maintenance kit for a rotary vane too) No idea about it longevity (at 10 years or more) Noisy
Pro Rotary vane
A very mature technology Better ultimate vacuum 15-20 years life expectancy Less expensive at purchase Less maintenance Really noiseless for some models
Contra
Oil pump, with all wellknown backstreaming problems Maintenance by a skilled mechanic, (or a good fiddler)
I would not hesitate to put a scroll on a SEM, with a today turbo pump. The price difference is nothing in comparison with the SEM. And if one has a maintenance contract, one has nothing to deal with these aspects.
About models, I would avoid the Varian SH100 which has much long term problems. It has been been replaced by the SH110, which seems to work well. The Edwards models are OK too. I don't know them from other manufacturer.
All models seems to need a few hours to go down to their ultimate vacuum.
No connections with the mentioned manufacturer, only comments from a critical user !
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Wharton.Sinkler-at-uop.com a écrit : } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } On the face of it, scroll pumps on an SEM would seem to be a great idea, replacing what is widely acknowledged to be the main source of hydrocarbon contamination (roughing pump oil), and thus avoiding the possibility of a single bad incident which can cause contamination for the remainder of an instrument's life. } } However, I'm a little concerned that the reliability of these pumps is not what it needs to be. What have others' experiences been - are scroll pumps a good or bad idea? Do the benefits of removing roughing pump oil outweigh the inconveniences, specifically not as good reliability as an oil roughing pump? Is there any concern using a scroll pump to back a diffusion pump, i.e. do these only make sense combined with a turbo? } } Are there particular models to avoid? And are there tricks to installing or handling them which will minimize trouble? } } Thanks, } Wharton } } } ************************************************************* } Wharton Sinkler, PhD. } UOP LLC } 50 E. Algonquin Rd. } Des Plaines IL 60017-5017 } mailto: wharton.sinkler-at-uop.com } tel 847-391-3878 } fax 847-391-3719 } } } } ==============================Original Headers============================== } 7, 27 -- From Wharton.Sinkler-at-uop.com Fri Sep 28 11:51:58 2007 } 7, 27 -- Received: from DE08IP008.honeywell.com (de08ip008.honeywell.com [199.64.94.25]) } 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l8SGpu3F005478 } 7, 27 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Sep 2007 11:51:57 -0500 } 7, 27 -- X-IronPort-AV: E=Sophos;i="4.21,210,1188802800"; } 7, 27 -- d="scan'208";a="69752" } 7, 27 -- Received: from de08cn851.honeywell.com (HELO DE08CN851.global.ds.honeywell.com) ([10.216.13.40]) } 7, 27 -- by DE08IP008.honeywell.com with ESMTP; 28 Sep 2007 09:52:16 -0700 } 7, 27 -- Received: from DE08EV804.global.ds.honeywell.com ([10.216.13.28]) by DE08CN851.global.ds.honeywell.com with Microsoft SMTPSVC(6.0.3790.3959); } 7, 27 -- Fri, 28 Sep 2007 12:51:55 -0400 } 7, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 7, 27 -- Content-class: urn:content-classes:message } 7, 27 -- MIME-Version: 1.0 } 7, 27 -- Content-Type: text/plain; } 7, 27 -- charset="iso-8859-1" } 7, 27 -- Subject: Scroll pumps, good or bad? } 7, 27 -- Date: Fri, 28 Sep 2007 12:51:55 -0400 } 7, 27 -- Message-ID: {25897AA5CCA4BF4D964E1807485029381EE92F-at-DE08EV804.global.ds.honeywell.com} } 7, 27 -- X-MS-Has-Attach: } 7, 27 -- X-MS-TNEF-Correlator: } 7, 27 -- Thread-Topic: Scroll pumps, good or bad? } 7, 27 -- Thread-Index: AcgB7+A43ruZdCVFRq+i5xCPJ6v/Ig== } 7, 27 -- From: "Sinkler, Wharton" {Wharton.Sinkler-at-uop.com} } 7, 27 -- To: {Microscopy-at-microscopy.com} } 7, 27 -- X-OriginalArrivalTime: 28 Sep 2007 16:51:55.0862 (UTC) FILETIME=[E0B1CF60:01C801EF] } 7, 27 -- Content-Transfer-Encoding: 8bit } 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l8SGpu3F005478 } ==============================End of - Headers============================== }
==============================Original Headers============================== 25, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Wed Oct 3 08:30:54 2007 25, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.151]) 25, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l93DUrWN002879 25, 29 -- for {microscopy-at-microscopy.com} ; Wed, 3 Oct 2007 08:30:54 -0500 25, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 25, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id l93DUnJY016285 25, 29 -- for {microscopy-at-microscopy.com} ; Wed, 3 Oct 2007 15:30:49 +0200 (CEST) 25, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 25, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 48ED3106E6BF 25, 29 -- for {microscopy-at-Microscopy.Com} ; Wed, 3 Oct 2007 15:29:41 +0200 (CEST) 25, 29 -- Message-ID: {47039953.4030003-at-ipcms.u-strasbg.fr} 25, 29 -- Date: Wed, 03 Oct 2007 15:29:55 +0200 25, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 25, 29 -- User-Agent: Thunderbird 1.5.0.13 (X11/20070824) 25, 29 -- MIME-Version: 1.0 25, 29 -- To: microscopy-at-microscopy.com 25, 29 -- Subject: Re: [Microscopy] Scroll pumps, good or bad? 25, 29 -- References: {200709281659.l8SGxHpn022997-at-ns.microscopy.com} 25, 29 -- In-Reply-To: {200709281659.l8SGxHpn022997-at-ns.microscopy.com} 25, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 25, 29 -- Content-Transfer-Encoding: 8bit 25, 29 -- X-IPCMS-MailScanner: Found to be clean 25, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 25, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.151]); Wed, 03 Oct 2007 15:30:49 +0200 (CEST) 25, 29 -- X-Virus-Scanned: ClamAV 0.88.7/4461/Wed Oct 3 10:50:48 2007 on mr1.u-strasbg.fr 25, 29 -- X-Virus-Status: Clean 25, 29 -- X-Spam-Status: No, score=-0.2 required=5.0 tests=AWL autolearn=disabled 25, 29 -- version=3.1.8 25, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr1.u-strasbg.fr ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jeff-at-tss-consulting.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jeff-at-tss-consulting.com Name: Jefrey A West
Organization: TSS Consulting Ltd
Title-Subject: [Filtered] TEM Sample Preparation
Question: Preparing Samples for TEM I am networking to people so that I can find candidates to fill a job preparing samples of semiconductor (single crystal) materials for TEM analysis in a local Corporation.
The person to fit the job I am engaged to fill will have experience with sample preparation for TEM or SEM with Semi- conductor types of materials. Currently the company is working with II-V and III-V materials and others.
Please pass on to anyone who you think might talk to me about this opportunity. Please contact me by email or by phone- your preference: Ph: 877-489-2425 Jeff
Phil is exactly right. Sputtering is a very dynamic process that will coat your samples with varying amounts of conductive metal depending on the parameters described. For our purposes in SEM we overlook many of these variables and think we are "controlling" the process but we really are not. I only need to use the uneven wear of the sputter target as an example of the un-evenness inherent in the process. The only way to get something close to a "reproducible thickness" is by a FTM (Film Thickness Monitor) which ignores the dynamics of the process described by Phil but calculates the amount of metal deposited on a Quartz Crystal, sort of a "Micro-balance" method. This method is not without its own flaws but is as good as it gets for our purposes. It's much more complex than I will go into but leaping through some of the hoops you've been describing will only get you stressed and not provide any meaningful results in the end. If FTM is not an option, best to do a sequential coating of a specimen till it looks good (Contrast/Resolution with minimal charging) in your microscope, then use those parameters as the starting point for future work.
P.S.
I'm sure Chuck Garber would have had much to say about this thread. I will miss him and his spin he put on the list server topics. He and I would sometimes "spar" privately regarding competitive issues. However, we always remained friendly and exchanged ideas about many concepts from time to time. RIP Chuck
Al Coritz Technical Engineer Electron Microscopy Sciences www.emsdiasum.com
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Wednesday, October 03, 2007 8:46 AM To: Sampleprep-at-earthlink.net
Andrea,
Ballpark, the thickness is roughly 10nm/minute in our Denton Desk II with gold or gold/palladium. But, this is strongly dependent on the gas pressure in the chamber, current, target (Au, Au/Pd, or something else) and target-sample distance, so that figure may not be relevant to your coater.
The second question, how thick for samples, is entirely dependent on 1) what kind of samples (how conductive are they, what's the surface topography [smooth, setose, etc.], 2) what accelerating voltage do you need (low voltage, less coating maybe), 3) how are the samples mounted? better the electrical mounts, therefore path to ground, can allow for thinner coats 4) what resolution (what magnification) do you need -- the higher the mag/resolution the thinner the coat required (thick of looking at a lawn after a the first snowfall. If the snow is light, it coats all the grass blades, but you can still see each individual blade and it's veins; if the snow is heavy, all you see is the snow. 5) Etc. So there's no simple answer to "how much?" It depends on "what questions?"
Phil
} This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } {http://www.microscopy.org/MicroscopyListserver/MLFormMail.htmlhttp://www.m icroscopy.org/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both {mailto:amgusman-at-ucdavis.eduamgusman-at-ucdavis.edu } as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: {mailto:amgusman-at-ucdavis.eduamgusman-at-ucdavis.edu } Name: Andrea Gusman } } Title-Subject: [Filtered] Sputter coater thickness } } Question: I am trying to determine how thick my sputter coater will } coat at different time intervals so I am conducting an experiment. I } made a small block out of Spurrs resin, sputter coated it, and then } placed the block back in the mold and covered the gold layer with } more Spurrs to sandwich it between resin. I then trimmed the block } and cross sectioned it on a microtome to obtain thin sections. I } placed the thin sections in the TEM and measured the gold layer. } The time intervals I used were 30 seconds, 90 seconds, 1 min, and 2 min. } Does anyone know how thick of a layer a sputter coater should coat } with a gold target at those time intervals? Or what is a suitable } thickness for SEM samples? } Also does anyone have any other suggestions on ways to determine } the thickness besides thin sectioning? } Thanks! } } } --------------------------------------------------------------------------- } } } -- } Andrea Gusman } Staff Research Associate I } Chemical Engineering & Material Science } University of California, Davis } 530-752-0284 -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859
==============================Original Headers============================== 5, 24 -- From oshel1pe-at-cmich.edu Wed Oct 3 07:45:44 2007 5, 24 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l93CjiHS010170 5, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Oct 2007 07:45:44 -0500 5, 24 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 24 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id l93D6B9b020590; 5, 24 -- Wed, 3 Oct 2007 09:06:25 -0400 5, 24 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 5, 24 -- Wed, 3 Oct 2007 08:45:34 -0400 5, 24 -- Mime-Version: 1.0 5, 24 -- Message-Id: {f06240805c3293bc8d850-at-[141.209.160.249]} 5, 24 -- In-Reply-To: {200710030154.l931sfkZ011872-at-ns.microscopy.com} 5, 24 -- References: {200710030154.l931sfkZ011872-at-ns.microscopy.com} 5, 24 -- Date: Wed, 3 Oct 2007 08:45:35 -0400 5, 24 -- To: amgusman-at-ucdavis.edu 5, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 24 -- Subject: Re: [Microscopy] MicroscopyListserverviaWWW: Sputter coater 5, 24 -- thickness 5, 24 -- Cc: Microscopy-at-microscopy.com 5, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 24 -- X-OriginalArrivalTime: 03 Oct 2007 12:45:34.0857 (UTC) FILETIME=[4A982F90:01C805BB] 5, 24 -- X-CanItPRO-Stream: default 5, 24 -- X-Spam-Score: -4 () L_EXCH_MF 5, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
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About Gold Coatings: Gold is not the best coating for SEM applications. I know that it has been used for a long time, but it goes down as a lumpy structure because of the way the film grows. It doesn't take much magnification before you start to see that structure in the SEM. Now if your sputter coater is the less expensive type with only a roughing pump, may I suggest that you use a Au-Pd target instead. That goes down a little more smoother than gold. It is still not the best coating to use for high resolution work, but it is better than gold. If you have a more high end sputter coating system, then go with other coatings such as Cr, Ir, or W.
About measuring the film thickness with the TEM: When you cut your samples, if you cut them parallel to the surface, you will compress the thickness of the films and it can give you an error. It is best to cut them perpendicular, i.e. on edge to avoid that. Also, you need to calibrate your TEM magnification carefully. I highly recommend the Mag-I-Cal sample that was developed by John McCaffrey. We sell that standard, but so do a lot of others. It is simply the best calibration standard for the TEM. When I was a PPG, we had to do a bi-annual calibration of the microscopes as part of the QS-9000 certification process. When I tried doing the calibration with two different standards to cover the entire magnification range, I found that the overlap range didn't match well. The Mag-I-Cal sample just worked great. I have even used it to calibrate a high resolution SEM at high magnifications. It is not as easy to do that with that sample, but I trusted the values better than other SEM standards at magnifications above 200,000X.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: amgusman-at-ucdavis.edu [mailto:amgusman-at-ucdavis.edu] Sent: Tuesday, October 02, 2007 6:54 PM To: Walck-at-SouthBayTech.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at {http://www.microscopy.org/MicroscopyListserver/MLFormMail.htmlhttp://www.mi croscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both {mailto:amgusman-at-ucdavis.eduamgusman-at-ucdavis.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: {mailto:amgusman-at-ucdavis.eduamgusman-at-ucdavis.edu Name: Andrea Gusman
Question: I am trying to determine how thick my sputter coater will coat at different time intervals so I am conducting an experiment. I made a small block out of Spurrs resin, sputter coated it, and then placed the block back in the mold and covered the gold layer with more Spurrs to sandwich it between resin. I then trimmed the block and cross sectioned it on a microtome to obtain thin sections. I placed the thin sections in the TEM and measured the gold layer. The time intervals I used were 30 seconds, 90 seconds, 1 min, and 2 min. Does anyone know how thick of a layer a sputter coater should coat with a gold target at those time intervals? Or what is a suitable thickness for SEM samples? Also does anyone have any other suggestions on ways to determine the thickness besides thin sectioning? Thanks!
Bitplane is expanding its US team and asks for applications for the following two positions. Feel free to forward this e-mail to anyone who might be interested.
Position #1 – Central and South Eastern Region Sales Representative US / Canada
Bitplane is looking for a biologist with strong computer skills and at least one year of hands-on experience using a confocal or similar 3D advanced light microscope. The duties of this position include: • Customer visits and analysis of customer's imaging needs. • Demonstration of the software and onsite work with the customer • Organization of exhibitions and workshops • Sales support of existing customers
The candidate is expected to have an outgoing personality with strong communication skills and should look forward to increased responsibility. At least 50% travel will be required. Representative is required to live in the territory they cover. Benefits include a base salary, performance based commission, 401K plan, healthcare, and vacation. Representative will work out of a home office. We offer a team of 18 people, fun to work with, and a truly international environment that provides the resources required to grow. We don't mind if the candidate does not have much business experience and we are prepared to show him/her the sales skills at the job.
Position #2 – Sales Development Coordinator
Bitplane is looking for a candidate with a science background and knowledge of the microscope community. The duties of this position include : • Identifying potential new regional customers via web searches, literature searches, marketing campaigns, trade shows, and customer referrals. • Introduction of Bitplane products and services to potential customers via email, phone, and Webex. • Understanding potential customers needs related to products offered by Bitplane. • Organizing workshops and demonstrations for regional sales representatives
The candidate is expected to have an outgoing personality, is detail oriented, articulates clearly, exhibits a high level of professionalism, and exceptional organization. Travel is not required. Benefits include a base salary, 401K plan, healthcare, and vacation. Representatives will work out of a home office and travel is not required on routine basis. We offer a team of 18 people, fun to work with, and a truly international environment that provides the resources required to grow.
Applicants should reply with a letter stating what position they are interested in, why they are qualified for the position, and enclose their CV.
With best regards, Mike
Bitplane Inc. Michael C. Wussow Vice President and General Manager Bitplane Inc.
Cell Phone: 651-336-4600 Fax: 866-691-9112 Toll Free: 1-888-3D-BITPX (332-4879) Visit Our Web Site At: www.bitplane.com
==============================Original Headers============================== 14, 32 -- From mike-at-bitplane.com Wed Oct 3 14:31:59 2007 14, 32 -- Received: from host55a.simplicato.com (host55a.simplicato.com [207.99.47.55]) 14, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l93JVxJt032085 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Oct 2007 14:31:59 -0500 14, 32 -- Received: from localhost (localhost.simplicato.com [127.0.0.1]) 14, 32 -- by host55a.simplicato.com (Postfix) with ESMTP id 09315B21772 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Oct 2007 15:31:59 -0400 (EDT) 14, 32 -- Received: from host55a.simplicato.com ([127.0.0.1]) 14, 32 -- by localhost (host55a.simplicato.com [127.0.0.1]) (amavisd-new, port 10024) 14, 32 -- with ESMTP id 13338-09 for {Microscopy-at-microscopy.com} ; 14, 32 -- Wed, 3 Oct 2007 15:31:58 -0400 (EDT) 14, 32 -- Received: from MikeLaptop (71-34-16-10.mpls.qwest.net [71.34.16.10]) 14, 32 -- by host55a.simplicato.com (Postfix) with ESMTP id BE30AB215E6 14, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Oct 2007 15:31:57 -0400 (EDT) 14, 32 -- Reply-To: {mike-at-bitplane.com} 14, 32 -- From: "Michael C. Wussow" {mike-at-bitplane.com} 14, 32 -- To: {Microscopy-at-microscopy.com} 14, 32 -- Subject: Bitplane Positions Available in the USA 14, 32 -- Date: Wed, 3 Oct 2007 14:31:51 -0500 14, 32 -- Organization: Bitplane Inc. 14, 32 -- Message-ID: {025d01c805f4$0d403550$27c09ff0$-at-com} 14, 32 -- MIME-Version: 1.0 14, 32 -- Content-Type: text/plain; 14, 32 -- charset="iso-8859-1" 14, 32 -- X-Mailer: Microsoft Office Outlook 12.0 14, 32 -- Thread-Index: AcgF8rLTv2vSJZEiQsuKjIWMfg4Mvg== 14, 32 -- Content-Language: en-us 14, 32 -- x-cr-hashedpuzzle: ED8= zWA= A0Wa A9e/ CJHO CJUc Clet DgQ6 Dn20 DrOQ E+9B FTRg FYc6 Frd4 GXJp Gt9z;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{9A7E5E82-EB2D-4F2F-809C-B2A9FA1B940A};bQBpAGsAZQBAAGIAaQB0AHAAbABhAG4AZQAuAGMAbwBtAA==;Wed, 03 Oct 2007 19:31:49 GMT;QgBpAHQAcABsAGEAbgBlACAAUABvAHMAaQB0AGkAbwBuAHMAIABBAHYAYQBpAGwAYQBiAGwAZQAgAGkAbgAgAHQAaABlACAAVQBTAEEA 14, 32 -- x-cr-puzzleid: {9A7E5E82-EB2D-4F2F-809C-B2A9FA1B940A} 14, 32 -- X-Virus-Scanned: by amavisd-new at simplicato.com 14, 32 -- Content-Transfer-Encoding: 8bit 14, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l93JVxJt032085 ==============================End of - Headers==============================
The University of Arizona's Arizona Research Labs (ARL) is seeking an outstanding candidate to serve as manager of the ARL Electron Microscopy Core Facility located at the Arizona Health Sciences Center (AHSC). The selected candidate will oversee day-to-day operations of the facility, select/maintain equipment, design techniques/protocols, and train/oversee users.
The AHSC and Main Campus Electron Microscopy Core Facilities are a University of Arizona resource. These facilities are equipped to provide both standard and specialized methods for transmission and scanning electron microscopy. The structure of the facilities is designed to accommodate the investigator who desires to undertake electron microscopy study. Projects are accepted with the approval of the facility manager.
Outstanding UA benefits include health, dental, vision, and life insurance; paid vacation, sick leave, and holidays; UA/ASU/NAU tuition reduction for the employee and qualified family members; access to UA recreation and cultural activities; state retirement; and more.
For more information please refer to Job #39315 on UA Career Track at www.uacareertrack.com/.
_____________________
David Elliott Ph.D. Assistant Professor - Department of Cell Biology and Anatomy Director, Research Microscopy Core Service University of Arizona College of Medicine PO Box 245004 Tucson, AZ 85724
Voice: 520-626-7870 Fax: 520-626-2097
==============================Original Headers============================== 8, 20 -- From Elliott-at-arizona.edu Wed Oct 3 15:45:03 2007 8, 20 -- Received: from smtpgate.email.arizona.edu (frodo.email.Arizona.EDU [128.196.133.168]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l93Kj3BM013489 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 3 Oct 2007 15:45:03 -0500 8, 20 -- Received: from frodos_amavis (amavis9.email.arizona.edu [10.0.0.237]) 8, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id E733523B706 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 3 Oct 2007 13:45:02 -0700 (MST) 8, 20 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 8, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 1847723B906 8, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 3 Oct 2007 13:45:01 -0700 (MST) 8, 20 -- Mime-Version: 1.0 (Apple Message framework v752.2) 8, 20 -- Content-Transfer-Encoding: 7bit 8, 20 -- Message-Id: {7B96F44F-172B-4672-BC88-446AFDB87A11-at-arizona.edu} 8, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed 8, 20 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 8, 20 -- From: David Elliott {Elliott-at-arizona.edu} 8, 20 -- Subject: University of Arizona EM job posting 8, 20 -- Date: Wed, 3 Oct 2007 13:44:58 -0700 8, 20 -- X-Mailer: Apple Mail (2.752.2) 8, 20 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
If you are not going to do a lot of quantitative microscopy measurements, then I would recommend that you buy the Image Processing Toolkit or Fovea Pro from Reindeer Graphics (http://www.reindeergraphics.com/). These are plug-ins that work in Adobe Photoshop. This is software that was written by John Russ and Chris Russ. It is worth it just for some of the image processing tools, but the quantitative microscopy tools are pretty good. It may not have the bells and whistles and may be a little slower because of working inside Photoshop, but it has some power. If you aren't going to be doing stereology measurements day in and day out it is a good add-on to Photoshop and much less expensive than some of the dedicated software packages out there. Another benefit is that there is a full tutorial on the package on the disk that comes with the software and you can buy John Russ' book that complements the software (or rather the software complements his book.)
Another point to make about this software is the level of help that you can get from the author. I'm not sure that he advertises this, but I've had some specific needs in the past where not only did John help me understand that concept, he wrote a macro or routine that was helped me do what I wanted to do. At PPG, we developed a quality test for one of our thin film products that was based on the help that John had given me. That's why I am a satisfied customer of this product.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: jhe1-at-tulane.edu [mailto:jhe1-at-tulane.edu] Sent: Tuesday, October 02, 2007 6:01 PM To: Walck-at-SouthBayTech.com
This Question was submitted to Ask-A-Microscopist by (jhe1-at-tulane.edu) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, October 2, 2007 at 10:58:31 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both jhe1-at-tulane.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: jhe1-at-tulane.edu Name: Jibao He
Organization: Tulane University
Education: Graduate College
Title: Image analysis software
Question: Dear Colleagues:
We are looking for image analysis software to process TEM and SEM images to get size distribution information. If you are using a good one, please recommend it to us including purchase contact information.
This Question was submitted to Ask-A-Microscopist by (ewgroth-at-gmail.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, October 3, 2007 at 16:04:45 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both ewgroth-at-gmail.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: ewgroth-at-gmail.com Name: Eric Roth
Organization: NYU
Education: Undergraduate College
Location: NY, NY
Title: Formvar Grids
Question: I've been attempting to make formvar grids. I've tried on three recent occasions and have been expereiencing a reoccurant artifact. The artifacts are about 1um and smaller, prefectly round, and white with a dark halo. Also, the smaller ones seem to be occuring in a linear pattern with the larger ones dispersed randomly. When I'm cleaning the glass I do so in a circular pattern with a kimwipe. In the reflection, it looks clean. I'm stumped as to what I'm doing wrong. Today, the formvar was pulling off really well and I paid special attention to cleaning the glass properly, but the grids turned out worse than ever. Any suggestions would be greatly appreciated. Thanks.
==============================Original Headers============================== 9, 11 -- From zaluzec-at-ultra5.microscopy.com Wed Oct 3 17:41:39 2007 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l93MfbsC008550 9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 3 Oct 2007 17:41:38 -0500 9, 11 -- Mime-Version: 1.0 9, 11 -- Message-Id: {p06240800c329cb041bf5-at-[206.69.208.22]} 9, 11 -- Date: Wed, 3 Oct 2007 17:41:36 -0500 9, 11 -- To: microscopy-at-microscopy.com 9, 11 -- From: ewgroth-at-gmail.com (by way of Ask-A-Microscopist) 9, 11 -- Subject: AskAMicroscopist: Making Formvar Grids 9, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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Hello, from time to time we have similar problems. The artifacts about 1um could be the holes. The small artifacts are are probably caused by some dirt that sticks on glass surface. You cannot see it by an eye.
Please, check: 1) The air humidity in the room in which, you are making the Formvar grids. It should be as low as possible.
2) The solvent (chloroform or dichlorethane) for making the working solution of Formvar should be dry, so minimize the content of water residues in it. We are keeping the solvent under molecular sieve or using freshly distilled one.
3) Let dissolve the Formvar powder is solvent for sufficient time to be sure, that all Formvar powder is well dissolved.
4) Keep the Formvar solution in clean, tightly closed glass flask in the dark.
Best regards from Prague Oldrich
------------------------------------------ Oldrich Benada Institute of Microbiology, Acad. Sci. CR Videnska 1083 142 20 Prague 4 - Krc Czech Republic
On 3 Oct 2007 at 17:44, ewgroth-at-gmail.com wrote:
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==============================Original Headers============================== 11, 24 -- From benada-at-biomed.cas.cz Thu Oct 4 01:41:14 2007 11, 24 -- Received: from biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l946fDKU002316 11, 24 -- for {microscopy-at-microscopy.com} ; Thu, 4 Oct 2007 01:41:14 -0500 11, 24 -- Received: from [147.231.44.101] (u117ob.mbu.cas.cz [147.231.44.101]) 11, 24 -- by biomed.cas.cz (8.13.4+Sun/8.13.3) with ESMTP id l946fBJx029417; 11, 24 -- Thu, 4 Oct 2007 08:41:11 +0200 (CEST) 11, 24 -- From: "Oldrich Benada" {benada-at-biomed.cas.cz} 11, 24 -- Organization: Institute of Microbiology 11, 24 -- To: ewgroth-at-gmail.com 11, 24 -- Date: Thu, 04 Oct 2007 08:41:07 +0200 11, 24 -- MIME-Version: 1.0 11, 24 -- Subject: Re: [Microscopy] AskAMicroscopist: Making Formvar Grids 11, 24 -- CC: microscopy-at-microscopy.com 11, 24 -- Message-ID: {4704A723.7052.2107CF-at-benada.biomed.cas.cz} 11, 24 -- Priority: normal 11, 24 -- In-reply-to: {200710032244.l93MiF5g012750-at-ns.microscopy.com} 11, 24 -- References: {200710032244.l93MiF5g012750-at-ns.microscopy.com} 11, 24 -- X-mailer: Pegasus Mail for Windows (4.41) 11, 24 -- Content-type: text/plain; charset=US-ASCII 11, 24 -- Content-transfer-encoding: 7BIT 11, 24 -- Content-description: Mail message body 11, 24 -- X-Antivirus: avast! (VPS 000778-2, 03.10.2007), Outbound message 11, 24 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
A few days ago, I asked the list about Phoenix TEM stainers. This is a summary of my answers.
I got two answers. Apparently, there are two new models of stainers for contrasting TEM sections on the market:
(i) Phoenix TEM stainers marketed from Germany by Reichert - Labtec. www.reichert-labtec.de (ii) There are other stainers, by Ted Pella, on the market now. www.tedpella.com
Warm thanks to both people who have answered me! If anybody has actual lab experience with either one of the stainers, I would be grateful for their comment.
Gerd
-- Dr. Gerd Leitinger
Institute of Cell Biology, Histology and Embryology Center for Molecular Medicine Medical University of Graz Harrachgasse 21 A-8010 Graz Austria
Can someone suggest a source for a multi-well mold to hold multiple tissue samples for agar embedment prior to processing for histology an LM examination? Can someone suggest a vendor to fabricate a mold if required? TIA
Walter F. Bobrowski Sr. Scientist Pfizer Global R&D 224 Eastern Point Rd B274/0357N Groton, CT 06340
TEL: 860-686-9426 FAX: 860-715-4919
==============================Original Headers============================== 5, 29 -- From Walter.Bobrowski-at-pfizer.com Thu Oct 4 07:11:47 2007 5, 29 -- Received: from mopmsgoa01.pfizer.com (mopmsgo.pfizer.com [148.168.100.84]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l94CBlZH004546 5, 29 -- for {microscopy-at-microscopy.com} ; Thu, 4 Oct 2007 07:11:47 -0500 5, 29 -- Received: from mopamrexc01.amer.pfizer.com (mopamrexc01.pfizer.com [170.116.32.254]) 5, 29 -- by mopmsgoa01.pfizer.com (8.13.7/8.13.7) with ESMTP id l94CBkoT007121 5, 29 -- for {microscopy-at-microscopy.com} ; Thu, 4 Oct 2007 08:11:46 -0400 5, 29 -- Received: from mopamrexc01.amer.pfizer.com ([170.116.33.201]) by mopamrexc01.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); 5, 29 -- Thu, 4 Oct 2007 08:11:46 -0400 5, 29 -- Received: from groamrexm03.amer.pfizer.com ([10.128.42.23]) by mopamrexc01.amer.pfizer.com with Microsoft SMTPSVC(6.0.3790.1830); 5, 29 -- Thu, 4 Oct 2007 08:11:46 -0400 5, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 5, 29 -- Content-class: urn:content-classes:message 5, 29 -- MIME-Version: 1.0 5, 29 -- Content-Type: text/plain; 5, 29 -- charset="us-ascii" 5, 29 -- Subject: Multi-well sample mold 5, 29 -- Date: Thu, 4 Oct 2007 08:11:43 -0400 5, 29 -- Message-ID: {7A20EEC75BBEE542B9E40B92F58E8C5305F95DA4-at-groamrexm03.amer.pfizer.com} 5, 29 -- X-MS-Has-Attach: 5, 29 -- X-MS-TNEF-Correlator: 5, 29 -- Thread-Topic: Multi-well sample mold 5, 29 -- thread-index: AcgGf7qDUL1JU/ZgTcyG6xcKwZBDkA== 5, 29 -- From: "Bobrowski, Walter" {Walter.Bobrowski-at-pfizer.com} 5, 29 -- To: {microscopy-at-microscopy.com} 5, 29 -- X-OriginalArrivalTime: 04 Oct 2007 12:11:46.0269 (UTC) FILETIME=[BBDFF8D0:01C8067F] 5, 29 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=4.65.5502:2.3.11,1.2.37,4.0.164 definitions=2007-10-04_03:2007-10-03,2007-10-04,2007-10-04 signatures=0 5, 29 -- Content-Transfer-Encoding: 8bit 5, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l94CBlZH004546 ==============================End of - Headers==============================
The artifacts are most likely holes as mentioned earlier. A method I've come across to eliminate them is to hold the slide just above the surface of the solvent for about twenty seconds or so after you've dunked it, allowing it to drip dry in the solvent vapors as opposed to drying directly in air.
Daryl Meyer UW-Madison
----- Original Message ----- X-from: ewgroth-at-gmail.com
Hello Micro Listers,
I wish to thank those who responded to my previous query of 9-25-07 on the resin infiltration problems I sometimes have when using an automated tissue processor for preparation of specimens for transmission electron microscopy. The information provided on the LR White acrylic resin problem was very helpful.
However, the infiltration problem I have encountered with some specimens when using an epoxy resin was addressed by only one respondent, who suggested I try the Spurr's epoxy resin for its lower viscosity. But I understand that one of its components, VCD, is no longer being manufactured, so I'm reluctant to use it (I do have a bottle of VCD, but its years old). I would like to solve this problem using an EPON type substitute. I have been using a Embed-812 formulation successfully for conventional and microwave non-automated processing for many years. However, due to increasing specimen processing loads, I would like to use the automated processor as much as possible.
1.Does anyone have an epoxy resin mixture that they are willing to share that gives good infiltration into specimens in the automated tissue processor?
2. More generally, does anyone have a complete protocol, fixation through resin infiltration, that works for use in a tissue processor for plant or animal tissues?
3. Are there specific processor conditions that you use for the resin infiltration steps, like agitation rates, time per resin change, temperature, etc. that you feel are important for good infiltration?
The details of my processor system, although important to consider, were in my previous post so I won't repeat them here.
Thanks for any helpful information you can give.
Gib -- Gilbert (Gib) Ahlstrand, Electron Microscopist, Imaging Center, University of Minnesota 123 Snyder Hall St. Paul, MN 55108 http://www.cbs.umn.edu/ic
==============================Original Headers============================== 9, 19 -- From ahlst007-at-umn.edu Thu Oct 4 09:47:53 2007 9, 19 -- Received: from mta-m2.tc.umn.edu (mta-m2.tc.umn.edu [134.84.119.106]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l94ElraC001214 9, 19 -- for {Microscopy-at-Microscopy.com} ; Thu, 4 Oct 2007 09:47:53 -0500 9, 19 -- Received: from [160.94.39.28] (x160-94-39-28.cbs.umn.edu [160.94.39.28]) 9, 19 -- by mta-m2.tc.umn.edu (UMN smtpd) with ESMTP 9, 19 -- for {Microscopy-at-Microscopy.com} ; Thu, 4 Oct 2007 09:47:52 -0500 (CDT) 9, 19 -- X-Umn-Remote-Mta: [N] x160-94-39-28.cbs.umn.edu [160.94.39.28] #+LO+TS+AU+HN 9, 19 -- Message-ID: {4704FCEC.3010304-at-umn.edu} 9, 19 -- Date: Thu, 04 Oct 2007 09:47:08 -0500 9, 19 -- From: Gib Ahlstrand {ahlst007-at-umn.edu} 9, 19 -- Reply-To: ahlst007-at-umn.edu 9, 19 -- Organization: Imaging Center UM 9, 19 -- User-Agent: Thunderbird 1.5.0.12 (Macintosh/20070509) 9, 19 -- MIME-Version: 1.0 9, 19 -- To: Microscopy-at-Microscopy.com 9, 19 -- Subject: Tissue processor revisited 9, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
My Failure Analysis group is acquiring a new high resolution FE SEM and have investigated three well known leaders in SEM engineering and manufacture. I transported the same samples to each performance demonstration to assure accurate comparison and analysis of the equipment. One of the microscopes obtained a series of SEI micrographs with layers in oxide otherwise not present in the images from the other SEMs. Subsequently, the presence of these layers was confirmed in BEI mode. Why are the layers present in the data from one SEM and not the others? I know signal detection varies based on parameters and geomerty of the microscope (solid/take-off angles, detector distance, working distance, ect.?), but does it vary enough in different instruments to this extreme?
Unfortunatley, I can't divulge any more information regarding the propietary sample! Thank you, in advance, for your time!
Regards, Marissa
==============================Original Headers============================== 4, 27 -- From mlibbee-at-gmail.com Thu Oct 4 11:30:38 2007 4, 27 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.229]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l94GUco5018120 4, 27 -- for {microscopy-at-microscopy.com} ; Thu, 4 Oct 2007 11:30:38 -0500 4, 27 -- Received: by wx-out-0506.google.com with SMTP id h30so228973wxd 4, 27 -- for {microscopy-at-microscopy.com} ; Thu, 04 Oct 2007 09:30:37 -0700 (PDT) 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 27 -- d=gmail.com; s=beta; 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- bh=Hx8vWrOx8RCTeyCiKQ4wFjUmL25a/uhM5r4QJ6zKiWM=; 4, 27 -- b=tNdJJbsDrQrKHXEQo0l1fj6igLtyP0fwBECVrmDKOjpHx/v1b18j6ZFu3jazO4S8o7PA5voeQ/4o0QMO55PlwUkdiDfaEzYccN5OvOqj3s/DPwhb+y5rmnzVFn36JaJNRHz2Js4g0s6fCRAvCX0awvj5njMoGsxnT8IiYROQMoc= 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 27 -- d=gmail.com; s=beta; 4, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- b=QFyg0dHCY9f2G82M0ZC4TAq+olzFeK6m/OS/EUJSt3nDmcvJ+F7qyh6gTomBKRE/BSopF2YXv5WfLxkUcp9zqGi030GhgAsrWVgACdXY9uUdWYFw6wb1va5XjGFG5haGoK7+3aDgY6Trx3bB0MtvK9Zqsq8gQyO3se2WNadJ9qA= 4, 27 -- Received: by 10.142.141.5 with SMTP id o5mr1957400wfd.1191515437066; 4, 27 -- Thu, 04 Oct 2007 09:30:37 -0700 (PDT) 4, 27 -- Received: by 10.143.162.7 with HTTP; Thu, 4 Oct 2007 09:30:37 -0700 (PDT) 4, 27 -- Message-ID: {a272d3fb0710040930j33420e7fq9dd5a7b68ddcce22-at-mail.gmail.com} 4, 27 -- Date: Thu, 4 Oct 2007 09:30:37 -0700 4, 27 -- From: "Marissa Libbee" {mlibbee-at-gmail.com} 4, 27 -- To: microscopy-at-microscopy.com 4, 27 -- Subject: Detection of Secondary Electrons 4, 27 -- MIME-Version: 1.0 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1 4, 27 -- Content-Transfer-Encoding: 7bit 4, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Can any one tell me difference between EMPA analysis using WD detector and SEM/WDS spot analysis. Would there be any significant differences in detection limits or precision?
Thank you,
Pavel Supervisor 216-692-5479 cell 216-402-8087 www.atclabs.com
==============================Original Headers============================== 7, 27 -- From PavelLozovyy-at-Eaton.com Thu Oct 4 11:37:41 2007 7, 27 -- Received: from tccbw1.etn.com (mail.eaton.com [192.104.67.6]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l94GbfDp026640 7, 27 -- for {Microscopy-at-Microscopy.Com} ; Thu, 4 Oct 2007 11:37:41 -0500 7, 27 -- Received: from cleohsgw01.napa.ad.etn.com (cleohsgw01.napa.ad.etn.com [151.110.126.182]) 7, 27 -- by tccbw1.etn.com (spamadmin-at-eaton.com) with ESMTP id 78FD4C7AFL 7, 27 -- for {Microscopy-at-Microscopy.Com} ; Thu, 4 Oct 2007 12:37:39 -0400 (EDT) 7, 27 -- Received: from CLEOHSMB03.napa.ad.etn.com ([151.110.40.71]) by cleohsgw01.napa.ad.etn.com with Microsoft SMTPSVC(6.0.3790.1830); 7, 27 -- Thu, 4 Oct 2007 12:33:24 -0400 7, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 27 -- Content-class: urn:content-classes:message 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Type: text/plain; 7, 27 -- charset="US-ASCII" 7, 27 -- Subject: RE: Welcome to the Microscopy ListServer:Info,Rules & FAQ 7, 27 -- Date: Thu, 4 Oct 2007 12:33:23 -0400 7, 27 -- Message-ID: {E142FDCECAA6BF429C7CE592AA3B95265E3530-at-CLEOHSMB03.napa.ad.etn.com} 7, 27 -- In-Reply-To: {200710041345.l94DjOb6019822-at-ns.microscopy.com} 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- Thread-Topic: Welcome to the Microscopy ListServer:Info,Rules & FAQ 7, 27 -- Thread-Index: AcgGjO8h3DbuhIC/TsS1vqfY6/fZ7AAFvsVQ 7, 27 -- From: {PavelLozovyy-at-Eaton.com} 7, 27 -- To: {Microscopy-at-Microscopy.Com} 7, 27 -- X-OriginalArrivalTime: 04 Oct 2007 16:33:24.0279 (UTC) FILETIME=[489E3470:01C806A4] 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l94GbfDp026640 ==============================End of - Headers==============================
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The most significant difference is the number of WD spectrometers - EPMA instruments can have up to 5 spectrometers with up to 4 crystals in each spectrometer. I'm not aware of any current SEM system that accomodates more than a single WD spectrometer. This means EPMA instruments can do a lot more WD analysis and generally do it a lot faster. The whole instrument is also designed for automated analyis over large areas and long periods of time. While SEMs can do the automated analysis, this capability is an add-on and not as sophisticated as EPMAs.
Other differences:
1. EPMA instruments tend to use vertical WD spectrometers while SEMs tend to use inclined spectrometers. This means that EPMA systems are much more sensitive to the Z position of the sample, which needs setting by light microscopy to withi a few microns. Inclined spectrometers are much less sensitive to Z height. Conversely, the vertical spectrometers remain in focus over a much wider X/Y area while inclined spectrometers go out of focus at the edges of the image for smaller regions.
2. EPMA instruments usually have very good stages, with good resolution and repeatability. Mapping and automated analysis is intended to be done over large areas by stage scanning, rather than beam scanning. This is more difficult with standard SEM stages.
WD spectrometer energy resolution, sensitivity, etc is much more a function of the spectrometer design rather than whether it is an EPMA or SEM.
In case you don't know, JEOL make SEMs and EPMA instruments :-)
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==============================Original Headers============================== 9, 16 -- From larry-at-cymru666.plus.com Thu Oct 4 14:47:47 2007 9, 16 -- Received: from ptb-relay02.plus.net (ptb-relay02.plus.net [212.159.14.213]) 9, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l94JlkRv027259 9, 16 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 4 Oct 2007 14:47:47 -0500 9, 16 -- Received: from [87.113.80.113] (helo=[192.168.1.2]) 9, 16 -- by ptb-relay02.plus.net with esmtp (Exim) id 1IdWfh-0004H4-3j; Thu, 04 Oct 2007 20:47:45 +0100 9, 16 -- Mime-Version: 1.0 9, 16 -- Message-Id: {p06240802c32afe9541f1-at-[192.168.1.2]} 9, 16 -- In-Reply-To: {200710041644.l94GikG8009099-at-ns.microscopy.com} 9, 16 -- References: {200710041644.l94GikG8009099-at-ns.microscopy.com} 9, 16 -- Date: Thu, 4 Oct 2007 21:47:21 +0100 9, 16 -- To: PavelLozovyy-at-Eaton.com, Microscopy-at-MSA.Microscopy.Com 9, 16 -- From: Larry Stoter {larry-at-cymru666.plus.com} 9, 16 -- Subject: [Microscopy] RE: Welcome to the Microscopy ListServer:Info,Rules 9, 16 -- & FAQ 9, 16 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
In my experience it is possible to find an instrument that handles one particular sample different to other similar instruments from competitive manufacturers.
kV, spot size, detector position(s), chamber accessories, but most importantly working distance all play their part. Some instruments are able in their upper detector (through the lens mode) to include a contribution obtained from converted backscatter which add BSE contrast to the image. This is usually achieved by adjusting the working distance allowing the BSE to strike components, generating the secondary's which are sucked up into the upper detector. In some instruments the difference between 3mm WD and 6mm WD can be quite staggering!
Do not panic it is probably real data you are picking up.
This is one reason why I insist on clients taking critical specimens to ALL the appropriate manufacturers for demonstrations, you never know what you will find and it is found to be on some occasions instrument specific.
Be happy you found this out.
Steve Chapman Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
----- Original Message ----- X-from: {mlibbee-at-gmail.com} To: {protrain-at-emcourses.com} Sent: Thursday, October 04, 2007 5:31 PM
Hi Listers, I'm looking for a part for a Cambridge/Leica/LEO S240 SEM. It's called an Ion Pump Light Baffle and it does two things: it shields any light that may come from the ion pump and also isolates the ion pump from the electron gun electrically. It looks exactly like the light baffle on a Varian Cold Cathode Ion Gage but only bigger. Carl Zeiss tells me that that they have none in stock and did not offer to build one. Anyone out there have this part lying around just collecting dust? I Google'd it, but nothing of any interest came up. Thanks in advance.
Gary Easton, Pres. Scanners Corporation Refurbished SEM Sales/ Service, Cambridge/Leica/LEO our specialty 410-857-7633
==============================Original Headers============================== 2, 21 -- From garyeaston-at-scannerscorp.com Thu Oct 4 15:54:25 2007 2, 21 -- Received: from omr11.networksolutionsemail.com (omr11.networksolutionsemail.com [205.178.146.61]) 2, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l94KsPeh022080 2, 21 -- for {microscopy-at-microscopy.com} ; Thu, 4 Oct 2007 15:54:25 -0500 2, 21 -- Received: from mail.networksolutionsemail.com (ns-omr11.mgt.hosting.dc2.netsol.com [10.49.6.74]) 2, 21 -- by omr11.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id l94KsPMh021419 2, 21 -- for {microscopy-at-microscopy.com} ; Thu, 4 Oct 2007 16:54:25 -0400 2, 21 -- Received: (qmail 20407 invoked by uid 78); 4 Oct 2007 20:54:24 -0000 2, 21 -- Received: from unknown (HELO ?127.0.0.1?) (70.22.31.104) 2, 21 -- by 10.49.36.74 with SMTP; 4 Oct 2007 20:54:24 -0000 2, 21 -- Message-ID: {4705610F.8090808-at-scannerscorp.com} 2, 21 -- Date: Thu, 04 Oct 2007 16:54:23 -0500 2, 21 -- From: "Gary M. Easton" {garyeaston-at-scannerscorp.com} 2, 21 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 2, 21 -- MIME-Version: 1.0 2, 21 -- To: Microscopy Society of America {microscopy-at-microscopy.com} 2, 21 -- Subject: CAMBRIDGE/LEICA/LEO SEM PART NEEDED 2, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 2, 21 -- Content-Transfer-Encoding: 7bit 2, 21 -- X-Antivirus: avast! (VPS 000778-3, 10/04/2007), Outbound message 2, 21 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
Good Morning: The Institution has decided to push wireless internet access into every corner of the campus. They also intend to boost the RF (cell phone) signal strength. I operate 3 TEMs, a LEO EFTEM, a cryo-Tecnai and an CM10. I worry that the TEMs will be affected by the signal frequency and strength from these new sources. I am being asked specific questions about which frequencies are harmful to our operation and am having trouble answering. If anyone has any experience or resources to help me communicate the scope of the problem it would be much appreciated. Thanks bob harris
Guelph Regional Integrated Imaging Facility (GRIIF) Transmission Electron Microscope Facility Dept. of Molecular and Cell Biology New Science Complex, 488 Gordon St. University of Guelph Guelph Ontario, Canada, N1G 2W1 Phone: 519-824-4120 X 56409 Fax: 519-837-1802
----- End forwarded message -----
Guelph Regional Integrated Imaging Facility (GRIIF) Transmission Electron Microscope Facility Dept. of Molecular and Cell Biology New Science Complex, 488 Gordon St. University of Guelph Guelph Ontario, Canada, N1G 2W1 Phone: 519-824-4120 X 56409 Fax: 519-837-1802
==============================Original Headers============================== 13, 27 -- From bharris-at-uoguelph.ca Fri Oct 5 07:45:52 2007 13, 27 -- Received: from mailhub.cs.uoguelph.ca (mailhub.cs.uoguelph.ca [131.104.94.205]) 13, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l95CjqY4032456 13, 27 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 07:45:52 -0500 13, 27 -- Received: from aragorn.cs.uoguelph.ca (css.webmail.uoguelph.ca [131.104.93.20]) 13, 27 -- by mailhub.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id l95Cjo1C009124 13, 27 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 08:45:50 -0400 13, 27 -- Received: from webmail.uoguelph.ca (localhost.localdomain [127.0.0.1]) 13, 27 -- by aragorn.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id l95CjoAr011307 13, 27 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 08:45:50 -0400 13, 27 -- Received: from 131.104.190.174 ([131.104.190.174]) by webmail.uoguelph.ca 13, 27 -- (Horde MIME library) with HTTP; Fri, 05 Oct 2007 08:45:50 -0400 13, 27 -- Message-ID: {20071005084550.ahwtgu68ycc4okos-at-webmail.uoguelph.ca} 13, 27 -- Date: Fri, 05 Oct 2007 08:45:50 -0400 13, 27 -- From: Robert J Harris {bharris-at-uoguelph.ca} 13, 27 -- To: microscopy-at-microscopy.com 13, 27 -- Subject: Wi-Fi and RF interference 13, 27 -- MIME-Version: 1.0 13, 27 -- Content-Type: text/plain; 13, 27 -- charset=ISO-8859-1; 13, 27 -- DelSp="Yes"; 13, 27 -- format="flowed" 13, 27 -- Content-Disposition: inline 13, 27 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) 13, 27 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.205 13, 27 -- Content-Transfer-Encoding: 8bit 13, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l95CjqY4032456 ==============================End of - Headers==============================
Please reply to list -- I'm in the same boat, except the IT people here don't care if they cause interference. They just chose 2.4 GHz for the WiFi, and if that interfers with cordless phones, it's your fault. Instruments? Not even a consideration to them. $*#^&$(#&(.
Phil
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==============================Original Headers============================== 4, 22 -- From oshel1pe-at-cmich.edu Fri Oct 5 08:05:00 2007 4, 22 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l95D50BS012416 4, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 08:05:00 -0500 4, 22 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 22 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id l95DPb9N012007 4, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 09:25:38 -0400 4, 22 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 4, 22 -- Fri, 5 Oct 2007 09:04:58 -0400 4, 22 -- Mime-Version: 1.0 4, 22 -- Message-Id: {f06240806c32be66fb196-at-[141.209.160.249]} 4, 22 -- In-Reply-To: {200710051253.l95CrvJt006754-at-ns.microscopy.com} 4, 22 -- References: {200710051253.l95CrvJt006754-at-ns.microscopy.com} 4, 22 -- Date: Fri, 5 Oct 2007 09:04:55 -0400 4, 22 -- To: Microscopy-at-microscopy.com 4, 22 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 22 -- Subject: Re: [Microscopy] Wi-Fi and RF interference 4, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 22 -- X-OriginalArrivalTime: 05 Oct 2007 13:04:58.0814 (UTC) FILETIME=[55316DE0:01C80750] 4, 22 -- X-CanItPRO-Stream: default 4, 22 -- X-Spam-Score: -3.2 () CELL_PHONE_IMPROVE,L_EXCH_MF 4, 22 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
I will remind you to please use Subject Titles in your messages that reflect the question/answer. If you are replying to a posting and the Subject Title needs changing to reflect this, please do so.
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Cheers,
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 5, 11 -- From zaluzec-at-microscopy.com Fri Oct 5 08:28:43 2007 5, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 5, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l95DSfUq025129 5, 11 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 08:28:43 -0500 5, 11 -- Mime-Version: 1.0 5, 11 -- Message-Id: {p06240801c32bebd5cf48-at-[206.69.208.22]} 5, 11 -- Date: Fri, 5 Oct 2007 08:28:41 -0500 5, 11 -- To: microscopy-at-microscopy.com 5, 11 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 5, 11 -- Subject: Administrivia: Subject Titles 5, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
Martin Storre from Nanofilm Technologie GmbH has given me new information on their TEM stainer, so I have to correct the summary I gave earlier.
Besides the Leica stainer, there is only one (and not two) new TEM grid stainer available. It was produced by Nanofilm Technologie GmbH Germany in cooperation with two scientists at the FU Berlin.
In the US, its distributor are Ted Pella; whereas in Germany, Austria, or Switzerland, it is distributed By Reichert Labtech.
I know that this has been addressed in various ways on this forum, but...
We are thinking of buying a set of the newer digital cameras to use with Nikon Eclipse scopes. We are looking for color accuracy, sufficient sensitivity for "bright" fluorescence, and simplicity of the software. We are considering either firewire of USB 2 connections, and CMOS or CCD models
To date, we have thought about the Nikon DSF1, Qimaging 3, the Jenoptik ProgRes CT3 or C#, the XLI-3, and the Lumenera infinity models.
Has anyone done a side-by-side evaluation of these models, or could suggest an additional one for consideration? It is probably best to answer off list.
Thank you very much.
Joel
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 9, 19 -- From jbs-at-temple.edu Fri Oct 5 10:29:41 2007 9, 19 -- Received: from imp1.temple.edu (imp1.ocis.temple.edu [155.247.166.81]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l95FTdhw022517 9, 19 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 10:29:41 -0500 9, 19 -- Received: from [155.247.98.40] (jbs.bio.temple.edu [155.247.98.40]) 9, 19 -- by imp1.temple.edu (8.12.3/8.11.3/SuSE Linux 8.11.1-0.5) with ESMTP id l95FTdwk028684 9, 19 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 11:29:39 -0400 9, 19 -- From: "Joel Sheffield" {jbs-at-temple.edu} 9, 19 -- To: microscopy-at-microscopy.com 9, 19 -- Date: Fri, 05 Oct 2007 11:30:20 -0400 9, 19 -- MIME-Version: 1.0 9, 19 -- Subject: Cameras 9, 19 -- Reply-to: jbs-at-temple.edu 9, 19 -- Message-ID: {4706204C.28108.4DE6E25B-at-jbs.temple.edu} 9, 19 -- Priority: normal 9, 19 -- X-mailer: Pegasus Mail for Windows (4.41) 9, 19 -- Content-type: text/plain; charset=US-ASCII 9, 19 -- Content-transfer-encoding: 7BIT 9, 19 -- Content-description: Mail message body ==============================End of - Headers==============================
Hi all, Happy Friday! There's a job opening here at the University of Georgia. Please reply to Michael Hahn {hahn-at-ccrc.uga.edu} if you are interested in this position. have a great week-end, Beth
Postdoctoral OR Senior Research Technician Position University of Georgia Complex Carbohydrate Research Center Microscopist A position is open at either the postdoctoral or senior research technician level to work on the immunolocalization of carbohydrate structures in the cell walls of agronomically important crop plants using an extensive library of monoclonal antibodies. Demonstrated experience with light (visible and fluorescence) and electron (TEM and SEM) microscopy is required; experience with laser confocal microscopy is desirable. All interested applicants should forward curriculum vitae and list of three references to: Michael Hahn, University of Georgia, Complex Carbohydrate Research Center, 315 Riverbend Road, Athens, GA 30602-4712; e-mail: hahn-at-ccrc.uga.edu; fax: 706-542-4412. Review of applications will begin September 15, 2007 and will continue until position is filled. The University of Georgia is an EEO/AA employer.
********************************************************************** Beth Richardson Electron Microscopy Lab Coordinator Plant Biology Department University of Georgia Athens, GA 30602-7271
http://www.plantbio.uga.edu/emlab/
Phone - (706) 542-1790 & FAX - (706) 542-1805
"Between the two evils, I always pick the one I never tried before". Mae West (1893-1980) *******************************************************************
"And it's only the giving that makes you what you are". Wond'ring Aloud, Jethro Tull (Aqualung)
************************************************************************ *** The Friends of the Marine Institute - Join Today! www.friendsofugami.org
==============================Original Headers============================== 11, 19 -- From beth-at-plantbio.uga.edu Fri Oct 5 16:39:15 2007 11, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l95LdE0C014421 11, 19 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 16:39:15 -0500 11, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 11, 19 -- (authenticated user beth-at-plantbio.uga.edu) 11, 19 -- by dogwood.plantbio.uga.edu 11, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)); 11, 19 -- Fri, 5 Oct 2007 17:38:41 -0400 11, 19 -- Mime-Version: 1.0 (Apple Message framework v752.3) 11, 19 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 11, 19 -- Message-Id: {47BB4823-413A-4944-B1A3-E51CE9F35D9D-at-plantbio.uga.edu} 11, 19 -- Cc: Michael Hahn {hahn-at-ccrc.uga.edu} 11, 19 -- Content-Transfer-Encoding: 7bit 11, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 11, 19 -- Subject: microscopy job 11, 19 -- Date: Fri, 5 Oct 2007 17:38:44 -0400 11, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 11, 19 -- X-Mailer: Apple Mail (2.752.3) ==============================End of - Headers==============================
Bob We did extensive testing on a lot of wireless devices, and the only interference we found was when a walkie-talkie was transmitting right next to the pre-amp of the EDX system, which smeared the EDX peaks badly. Aside from that, there was no effect on the electron beam (that was for a 2010F, but the SEM guys did similar testing and found no effect, so don't worry.
John Mardinly Intel This is not an opinion of Intel Corporation.
-----Original Message----- X-from: bharris-at-uoguelph.ca [mailto:bharris-at-uoguelph.ca] Sent: Friday, October 05, 2007 5:46 AM To: Mardinly, John
Good Morning: The Institution has decided to push wireless internet access into every corner of the campus. They also intend to boost the RF (cell phone) signal strength. I operate 3 TEMs, a LEO EFTEM, a cryo-Tecnai and an CM10. I worry that the TEMs will be affected by the signal frequency and strength from these new sources. I am being asked specific questions about which frequencies are harmful to our operation and am having trouble answering. If anyone has any experience or resources to help me communicate the scope of the problem it would be much appreciated. Thanks bob harris
Guelph Regional Integrated Imaging Facility (GRIIF) Transmission Electron Microscope Facility Dept. of Molecular and Cell Biology New Science Complex, 488 Gordon St. University of Guelph Guelph Ontario, Canada, N1G 2W1 Phone: 519-824-4120 X 56409 Fax: 519-837-1802
----- End forwarded message -----
Guelph Regional Integrated Imaging Facility (GRIIF) Transmission Electron Microscope Facility Dept. of Molecular and Cell Biology New Science Complex, 488 Gordon St. University of Guelph Guelph Ontario, Canada, N1G 2W1 Phone: 519-824-4120 X 56409 Fax: 519-837-1802
==============================Original Headers============================== 13, 27 -- From bharris-at-uoguelph.ca Fri Oct 5 07:45:52 2007 13, 27 -- Received: from mailhub.cs.uoguelph.ca (mailhub.cs.uoguelph.ca [131.104.94.205]) 13, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l95CjqY4032456 13, 27 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 07:45:52 -0500 13, 27 -- Received: from aragorn.cs.uoguelph.ca (css.webmail.uoguelph.ca [131.104.93.20]) 13, 27 -- by mailhub.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id l95Cjo1C009124 13, 27 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 08:45:50 -0400 13, 27 -- Received: from webmail.uoguelph.ca (localhost.localdomain [127.0.0.1]) 13, 27 -- by aragorn.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id l95CjoAr011307 13, 27 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 08:45:50 -0400 13, 27 -- Received: from 131.104.190.174 ([131.104.190.174]) by webmail.uoguelph.ca 13, 27 -- (Horde MIME library) with HTTP; Fri, 05 Oct 2007 08:45:50 -0400 13, 27 -- Message-ID: {20071005084550.ahwtgu68ycc4okos-at-webmail.uoguelph.ca} 13, 27 -- Date: Fri, 05 Oct 2007 08:45:50 -0400 13, 27 -- From: Robert J Harris {bharris-at-uoguelph.ca} 13, 27 -- To: microscopy-at-microscopy.com 13, 27 -- Subject: Wi-Fi and RF interference 13, 27 -- MIME-Version: 1.0 13, 27 -- Content-Type: text/plain; 13, 27 -- charset=ISO-8859-1; 13, 27 -- DelSp="Yes"; 13, 27 -- format="flowed" 13, 27 -- Content-Disposition: inline 13, 27 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) 13, 27 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.205 13, 27 -- Content-Transfer-Encoding: 8bit 13, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l95CjqY4032456 ==============================End of - Headers==============================
==============================Original Headers============================== 20, 34 -- From john.mardinly-at-intel.com Fri Oct 5 19:44:27 2007 20, 34 -- Received: from mga09.intel.com (mga09.intel.com [134.134.136.24]) 20, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l960iRt4001642 20, 34 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 5 Oct 2007 19:44:27 -0500 20, 34 -- Received: from fmsmga002.fm.intel.com ([10.253.24.26]) 20, 34 -- by orsmga102.jf.intel.com with ESMTP; 05 Oct 2007 17:44:26 -0700 20, 34 -- X-ExtLoop1: 1 20, 34 -- X-IronPort-AV: E=Sophos;i="4.21,238,1188802800"; 20, 34 -- d="scan'208";a="164150578" 20, 34 -- Received: from fmsmsx334.amr.corp.intel.com ([132.233.42.1]) 20, 34 -- by fmsmga002.fm.intel.com with ESMTP; 05 Oct 2007 17:44:26 -0700 20, 34 -- Received: from scsmsx412.amr.corp.intel.com ([10.3.90.31]) by fmsmsx334.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 20, 34 -- Fri, 5 Oct 2007 17:44:26 -0700 20, 34 -- Received: from scsmsx415.amr.corp.intel.com ([10.3.90.34]) by scsmsx412.amr.corp.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 20, 34 -- Fri, 5 Oct 2007 17:44:25 -0700 20, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 20, 34 -- Content-class: urn:content-classes:message 20, 34 -- MIME-Version: 1.0 20, 34 -- Content-Type: text/plain; 20, 34 -- charset="us-ascii" 20, 34 -- Subject: RE: [Microscopy] Wi-Fi and RF interference 20, 34 -- Date: Fri, 5 Oct 2007 17:44:25 -0700 20, 34 -- Message-ID: {F3CB8931ABF8294DB889E977150CAD6801357B43-at-scsmsx415.amr.corp.intel.com} 20, 34 -- In-Reply-To: {200710051246.l95Ck2Id032572-at-ns.microscopy.com} 20, 34 -- X-MS-Has-Attach: 20, 34 -- X-MS-TNEF-Correlator: 20, 34 -- Thread-Topic: [Microscopy] Wi-Fi and RF interference 20, 34 -- Thread-Index: AcgHTbCZC9trtBLWQcyHgQEiS1gyHAAY7hBA 20, 34 -- References: {200710051246.l95Ck2Id032572-at-ns.microscopy.com} 20, 34 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 20, 34 -- To: {bharris-at-uoguelph.ca} , {Microscopy-at-msa.microscopy.com} 20, 34 -- X-OriginalArrivalTime: 06 Oct 2007 00:44:25.0885 (UTC) FILETIME=[0B8444D0:01C807B2] 20, 34 -- Content-Transfer-Encoding: 8bit 20, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l960iRt4001642 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both corvos-at-aol.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: corvos-at-aol.com Name: Walter Protheroe
Organization: E-MAC, Inc.
Title-Subject: [Filtered] SEM - LEO 1525 service and parts
Question: I have a customer in the Houston, TX area looking for an independent service organization to take over his LEO 1525. Preferably base no further than the Dallas area if possible. Also, I would like inquires from individuals who can also supply parts for the LEO 1525.
Any help or recommendations from users would be passed along...
Please correct me if I am wrong, but doesn't the FCC regulate the frequencies that may be used by different devices?
Is it possible to shield your instruments, perhaps with a Faraday cage? A partial cage made of wire mesh stapled onto movable partitions (grounded together) - like the partitions that divide office space into cubicles might even do the job. One would need to know the minimum mesh size of hardware cloth needed to block that frequency.
Take care, Bob Carter
Lopez Island, WA
----- Original Message ----- X-from: {oshel1pe-at-cmich.edu} To: {bob-at-rockisland.com} Sent: Friday, October 05, 2007 6:05 AM
==============================Original Headers============================== 9, 31 -- From bob-at-rockisland.com Mon Oct 8 09:37:36 2007 9, 31 -- Received: from mars.rockisland.com (lists.rockisland.com [64.119.0.12]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l98EbZSu018685 9, 31 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 09:37:35 -0500 9, 31 -- Received: from localhost (localhost [127.0.0.1]) 9, 31 -- by localhost.rockisland.com (Postfix) with ESMTP id 2A23C5A91 9, 31 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 07:37:35 -0700 (PDT) 9, 31 -- X-Virus-Scanned: amavisd-new at rockisland.com 9, 31 -- Received: from mars.rockisland.com ([127.0.0.1]) 9, 31 -- by localhost (smtp.rockisland.com [127.0.0.1]) (amavisd-new, port 10024) 9, 31 -- with ESMTP id GZCz+hWHYgwd for {microscopy-at-microscopy.com} ; 9, 31 -- Mon, 8 Oct 2007 07:37:34 -0700 (PDT) 9, 31 -- Received: from housecat (adsl-sj-11-46.rockisland.net [64.119.11.46]) 9, 31 -- by mars.rockisland.com (Postfix) with SMTP id A0BF85A8A 9, 31 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 07:37:34 -0700 (PDT) 9, 31 -- Message-ID: {001f01c809b9$5e70f540$0202a8c0-at-housecat} 9, 31 -- From: "Bob Carter" {bob-at-rockisland.com} 9, 31 -- To: "Microscopy - Plain Text" {microscopy-at-microscopy.com} 9, 31 -- References: {200710051305.l95D5oYp014068-at-ns.microscopy.com} 9, 31 -- Subject: Re: Re: Wi-Fi and RF interference 9, 31 -- Date: Mon, 8 Oct 2007 07:41:53 -0700 9, 31 -- MIME-Version: 1.0 9, 31 -- Content-Type: text/plain; 9, 31 -- format=flowed; 9, 31 -- charset="Windows-1252"; 9, 31 -- reply-type=original 9, 31 -- Content-Transfer-Encoding: 7bit 9, 31 -- X-Priority: 3 9, 31 -- X-MSMail-Priority: Normal 9, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 9, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 ==============================End of - Headers==============================
We have had problem in recent years with contamination on our TEM. As a consequence, we have to change objective aperture strip as frequent as every 4-5 months. We use liquid nitrogen daily. We clean film plates periodically. We minimize the time when we have to open the camera chamber to change films. But the problem persists.
I suspect the reason we are getting more contamination than years ago when we only looked at resin embedded samples on scope is that we have had increasing number of users who look at nanoparticles. Unlike biological material, these nano particles may not inhere to support film as well therefore may “fly” away when beam hits them. This problem can be worse when particles are not in a single layer on support film. Also, users often do negative staining of these nanoparticles in order to see the coating. I assume too much PTA or UA dried onto the grids can contribute to the problem as well.
We use our scope at 75 KV and usually use 10 or 20µm objective apertures for adequate contrast. This means there are only 1 or 2 apertures we can use in a standard objective aperture strip. Objective aperture strips are very expensive (about $1300/ea). Obviously we cannot use them as disposable items so often.
My questions are:
1. Do you have many users looking at nanoparticles or negatively stained materials on your scope, If yes, how long do you think I should expect an objective aperture to last based on your experience. 2. Is there anything else we should do to minimize contamination and prolong the life of objective apertures.
Thank you very much in advance.
Hong Emory SOM EM
==============================Original Headers============================== 10, 20 -- From hyi-at-emory.edu Mon Oct 8 10:19:14 2007 10, 20 -- Received: from mr5.cc.emory.edu (mr5.cc.emory.edu [170.140.52.94]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l98FJCiw032187 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 10:19:13 -0500 10, 20 -- Received: from emory.edu (emoryfloatdmz.cc.emory.edu [170.140.52.254]) 10, 20 -- by mr5.cc.emory.edu (8.13.1/8.13.1) with ESMTP id l98FJ6H8008333 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 11:19:06 -0400 10, 20 -- Date: Mon, 8 Oct 2007 11:22:42 -0400 10, 20 -- Mime-Version: 1.0 (Apple Message framework v553) 10, 20 -- Content-Type: text/plain; charset=WINDOWS-1252; format=flowed 10, 20 -- Subject: Contamination on TEM 10, 20 -- From: Hong Yi {hyi-at-emory.edu} 10, 20 -- To: microscopy-at-microscopy.com 10, 20 -- Message-Id: {50654733-75B2-11DC-AEFE-0003939EF2BC-at-emory.edu} 10, 20 -- X-Mailer: Apple Mail (2.553) 10, 20 -- X-emory.edu-MailScanner: Found to be clean 10, 20 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu 10, 20 -- X-Spam-Status: No 10, 20 -- Content-Transfer-Encoding: 8bit 10, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l98FJCiw032187 ==============================End of - Headers==============================
I work in a very "RF rich" environment that not only includes 802.11x in every corner of the campus, but every cell phone air interface (RF transmit/receive channel) in use today anywhere in the world. We have two FE-SEMs and three FIB tools in our lab, and have never observed any interference from RF sources (60Hz power lines are another story...).
Alan Street Senior Staff Support Engineer/Manager Focused Ion Beam/Failure Analysis Services Qualcomm CDMA Technologies 5775 Morehouse Drive San Diego, CA 92121 USA astreet-at-qualcomm.com
On 05 Oct 2007, at 2:55 PM, bharris-at-uoguelph.ca wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } } Good Morning: The Institution has decided to push wireless internet } access into every corner of the campus. They also intend to boost the } RF (cell phone) signal strength. I operate 3 TEMs, a LEO EFTEM, a } cryo-Tecnai and an CM10. I worry that the TEMs will be affected by the } signal frequency and strength from these new sources. I am being asked } specific questions about which frequencies are harmful to our } operation and am } having trouble answering. If anyone has any experience or resources to } help me communicate the scope of the problem it would be much } appreciated. Thanks bob harris } } Guelph Regional Integrated Imaging Facility (GRIIF) } Transmission Electron Microscope Facility } Dept. of Molecular and Cell Biology } New Science Complex, 488 Gordon St. } University of Guelph } Guelph Ontario, Canada, N1G 2W1 } Phone: 519-824-4120 X 56409 } Fax: 519-837-1802 } } } } } ----- End forwarded message ----- } } } Guelph Regional Integrated Imaging Facility (GRIIF) } Transmission Electron Microscope Facility } Dept. of Molecular and Cell Biology } New Science Complex, 488 Gordon St. } University of Guelph } Guelph Ontario, Canada, N1G 2W1 } Phone: 519-824-4120 X 56409 } Fax: 519-837-1802 } } } } } } ==============================Original } Headers============================== } 13, 27 -- From bharris-at-uoguelph.ca Fri Oct 5 07:45:52 2007 } 13, 27 -- Received: from mailhub.cs.uoguelph.ca } (mailhub.cs.uoguelph.ca [131.104.94.205]) } 13, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l95CjqY4032456 } 13, 27 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 } 07:45:52 -0500 } 13, 27 -- Received: from aragorn.cs.uoguelph.ca } (css.webmail.uoguelph.ca [131.104.93.20]) } 13, 27 -- by mailhub.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id } l95Cjo1C009124 } 13, 27 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 } 08:45:50 -0400 } 13, 27 -- Received: from webmail.uoguelph.ca (localhost.localdomain } [127.0.0.1]) } 13, 27 -- by aragorn.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id } l95CjoAr011307 } 13, 27 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 } 08:45:50 -0400 } 13, 27 -- Received: from 131.104.190.174 ([131.104.190.174]) by } webmail.uoguelph.ca } 13, 27 -- (Horde MIME library) with HTTP; Fri, 05 Oct 2007 } 08:45:50 -0400 } 13, 27 -- Message-ID: } {20071005084550.ahwtgu68ycc4okos-at-webmail.uoguelph.ca} } 13, 27 -- Date: Fri, 05 Oct 2007 08:45:50 -0400 } 13, 27 -- From: Robert J Harris {bharris-at-uoguelph.ca} } 13, 27 -- To: microscopy-at-microscopy.com } 13, 27 -- Subject: Wi-Fi and RF interference } 13, 27 -- MIME-Version: 1.0 } 13, 27 -- Content-Type: text/plain; } 13, 27 -- charset=ISO-8859-1; } 13, 27 -- DelSp="Yes"; } 13, 27 -- format="flowed" } 13, 27 -- Content-Disposition: inline } 13, 27 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) } 13, 27 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.205 } 13, 27 -- Content-Transfer-Encoding: 8bit } 13, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id l95CjqY4032456 } ==============================End of - } Headers==============================
==============================Original Headers============================== 8, 23 -- From astreet-at-qualcomm.com Mon Oct 8 10:45:02 2007 8, 23 -- Received: from numenor.qualcomm.com (numenor.qualcomm.com [129.46.51.58]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l98Fj2qH012568 8, 23 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 10:45:02 -0500 8, 23 -- Received: from hamtaro.qualcomm.com (hamtaro.qualcomm.com [129.46.61.157]) 8, 23 -- by numenor.qualcomm.com (8.13.6/8.12.5/1.0) with ESMTP id l98Fj0mt027334 8, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=FAIL) 8, 23 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 08:45:01 -0700 8, 23 -- Received: from [192.168.1.20] (vpn-10-50-0-42.qualcomm.com [10.50.0.42]) 8, 23 -- by hamtaro.qualcomm.com (8.13.6/8.13.6/1.0) with ESMTP id l98FivhY008348 8, 23 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 8, 23 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 08:44:59 -0700 (PDT) 8, 23 -- Mime-Version: 1.0 (Apple Message framework v752.3) 8, 23 -- In-Reply-To: {200710051255.l95CtC8c008569-at-ns.microscopy.com} 8, 23 -- References: {200710051255.l95CtC8c008569-at-ns.microscopy.com} 8, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 8, 23 -- Message-Id: {551AE5EA-2EE3-4652-BB46-2043E9477663-at-qualcomm.com} 8, 23 -- Content-Transfer-Encoding: 7bit 8, 23 -- From: Alan Street {astreet-at-qualcomm.com} 8, 23 -- Subject: Re: [Microscopy] Wi-Fi and RF interference 8, 23 -- Date: Mon, 8 Oct 2007 17:44:21 +0200 8, 23 -- To: microscopy-at-microscopy.com 8, 23 -- X-Mailer: Apple Mail (2.752.3) ==============================End of - Headers==============================
Well, I guess if the solution is partitions and wire mesh etc., to get WiFi with no interference, or just use our present ethernet...I think I'll stick to ethernet! :-)
Larry
At 9:40 AM -0500 10/8/07, bob-at-rockisland.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- Dr. Lawrence F. Allard Distinguished Research Staff Member High Temperature Materials Laboratory Microscopy Group Materials Science and Technology Division Oak Ridge National Laboratory 1 Bethel Valley Road PO Box 2008 Oak Ridge, TN 37831-6064 (note: the last 4 lines are sufficient for mailing or overnight courier service) 865-574-4981 (office) 865-607-1144 (cell) 865-576-5413 (fax) http://www.ms.ornl.gov/htmlhome/mauc
==============================Original Headers============================== 8, 25 -- From allardlfjr-at-ornl.gov Mon Oct 8 12:51:21 2007 8, 25 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l98HpKcU029458 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 12:51:20 -0500 8, 25 -- Received: from emroute3.ornl.gov ([127.0.0.1]) 8, 25 -- by emroute3.ornl.gov (PMDF V6.3-x11 #31501) 8, 25 -- with ESMTP id {0JPL001IZTLJLL-at-emroute3.ornl.gov} for 8, 25 -- microscopy-at-microscopy.com; Mon, 08 Oct 2007 13:51:20 -0400 (EDT) 8, 25 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov 8, 25 -- (PMDF V6.3-x11 #31501) id {0JPL00301TLJ65-at-emroute3.ornl.gov} ; Mon, 8, 25 -- 08 Oct 2007 13:51:19 -0400 (EDT) 8, 25 -- Received: from [160.91.159.154] (allardlfjrpb.ornl.gov [160.91.159.154]) 8, 25 -- by emroute3.ornl.gov (PMDF V6.3-x11 #31501) 8, 25 -- with ESMTP id {0JPL0023BTLIWC-at-emroute3.ornl.gov} ; Mon, 8, 25 -- 08 Oct 2007 13:51:19 -0400 (EDT) 8, 25 -- Date: Mon, 08 Oct 2007 13:51:15 -0400 8, 25 -- From: Larry Allard {allardlfjr-at-ornl.gov} 8, 25 -- Subject: Re: [Microscopy] Wi-Fi and RF interference 8, 25 -- In-reply-to: {200710081440.l98Ee64k021155-at-ns.microscopy.com} 8, 25 -- To: bob-at-rockisland.com 8, 25 -- Cc: microscopy-at-microscopy.com 8, 25 -- Message-id: {p06230905c3301e3dc2f2-at-[160.91.159.154]} 8, 25 -- MIME-version: 1.0 8, 25 -- Content-type: text/plain; format=flowed; charset=us-ascii 8, 25 -- References: {200710081440.l98Ee64k021155-at-ns.microscopy.com} ==============================End of - Headers==============================
Your EM column and the electronics are encased in metal parts and cabinets that are grounded, I hope. A Faraday cage is a shield. Shielding is used to contain or keep out electric fields and RF. So your EM is a nearly perfect Faraday Shield, if properly grounded to the earth over a low resistance path to a driven earth ground.
The comments from John and Alan state that they don't see the problem. I can see why.
Here's a little paradox of sorts. How come a wireless RF transceiver right next to your PC is not interferring with your PC but it does with your grounded EM thirty feet away? The FCC and manufacturers are a few shielding steps ahead of us. Even the inside surface of a plastic PC case is metalized and grounded to reduce interference.
JMO,
Paul Amateur Radio Station KC8O, extra class and commercial FCC license holder for over thirty years.
At 09:38 AM 10/8/07 -0500, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 7, 26 -- From beaurega-at-westol.com Mon Oct 8 13:40:09 2007 7, 26 -- Received: from smtp-gateway-5.winbeam.com (smtp-gateway-5.winbeam.com [64.84.97.70]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l98Ie7Li010541 7, 26 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 13:40:09 -0500 7, 26 -- X-Winbeam-MailScanner-Watermark: 1192473512.59247-at-4MFKYxQxXUEMEqqOknAIeg 7, 26 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 7, 26 -- by smtp-gateway-5.winbeam.com (8.13.2/8.12.8) with SMTP id l98IcTd2013027 7, 26 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 14:38:30 -0400 7, 26 -- Received: (qmail 8238 invoked by uid 89); 8 Oct 2007 18:38:21 -0000 7, 26 -- Received: from pitts-69-72-22-210.dynamic-dialup.coretel.net (HELO beaurega) (69.72.22.210) 7, 26 -- by mail.winbeam.com with SMTP; 8 Oct 2007 18:38:21 -0000 7, 26 -- Message-Id: {3.0.6.32.20071008143956.007e44a0-at-pop3.norton.antivirus} 7, 26 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus 7, 26 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 7, 26 -- Date: Mon, 08 Oct 2007 14:39:56 -0400 7, 26 -- To: microscopy-at-microscopy.com 7, 26 -- From: Beaurega {beaurega-at-westol.com} 7, 26 -- Subject: Re: [Microscopy] Wi-Fi and RF interference 7, 26 -- In-Reply-To: {200710081438.l98EcSej019408-at-ns.microscopy.com} 7, 26 -- Mime-Version: 1.0 7, 26 -- Content-Type: text/plain; charset="us-ascii" 7, 26 -- X--MailScanner-Information: - Please contact Technical Support for more information 7, 26 -- X--MailScanner: Found to be clean (courtesy of MailScanner) 7, 26 -- X--MailScanner-SpamCheck: not spam (whitelisted), SpamAssassin (not cached, 7, 26 -- timed out) 7, 26 -- X--MailScanner-From: beaurega-at-westol.com ==============================End of - Headers==============================
The aperture strips just simply do need cleaning or replacing at intervals. Where you are using very small apertures the problem will get to a significant level much more quickly. I think that something like a 60um obj aperture is fairly common with 60-80kV and most microscopes, but there are so many variables and designs I assume you need the small apertures for your work. But they will need more frequent cleaning or replacement.
We get 2 routine services a year as part of our service contracts. All apertures, fixed and strips, get changed at least once a year - and basically need it; if we had heavier usage we would need to do this at both intervals. The CL and OL strips get changed more frequently as needed, by me. We use a 120/60/60/60 (um) OL strip in our old JEOL 100S, almost entirely used with a 60um choice. The strips are Molybdenum, and I clean them in our vacuum evaporator (ancient, Kinney KDP-2 ODP model), heating in a tungsten boat to a "bright orange" heat for a few minutes at ~ 5x10-5 torr; too hot and you will melt the strip to the boat. Be careful about any exact Voltage/Current recommendations and go by visual color. The exact values will depend on your unit and the particular boat (length, width, gage, etc). Experiment and err to the low side, checking the apertures to see if they were cleaned and not melting to the boat. It used to be expected that the apertures would be cleaned, and the info was in the scope manuals.... This is not some substandard operation. I think the service engineers had problems in using client evaporators reliably (very understandable) and so THEY quit cleaning, and most people don't do it themselves and just pay for the new ones now.
I have been using a few strips for over 12 yr. I also "burn" the fixed CL discs the same. The vendor service engineer tells me that I am about the only one who cleans the apertures anymore, but the results are good and resolution tests are fine so it is obviously possible to do this. Again, this used to be the norm....
If you do this, caution the service engineer ahead of time to be careful removing the apertures to avoid damage. Actually some of my strips are fairly wrinkled (have been bent and smoothed out...) but work like new ones. They DO need to be flat and have non-deformed aperture edges, but the cosmetics do not matter otherwise.
You may have problems with vendors not wanting to do this; if there is a problem in the fixed CL discs it it much trouble to change; you have to understand their position. But if you have a spare set cleaned that look good there should be no problem; check them carefully under a dissecting scope for roundness and contamination. The strips are easily changed yourself so it should be no issue. But you might want to keep a new strip on hand; if there are beam/image problems they may blame your apertures and insist on new ones even if that is not the problem. I have NOT had this problem - our JEOL engineers tolerate my quirky ways and it all works fine.
Hope this helps.
Dale Callaham UMASS Amherst
hyi-at-emory.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Microscopy Researchers: } } We have had problem in recent years with contamination on our TEM. As } a consequence, we have to change objective aperture strip as frequent } as every 4-5 months. We use liquid nitrogen daily. We clean film } plates periodically. We minimize the time when we have to open the } camera chamber to change films. But the problem persists. } } I suspect the reason we are getting more contamination than years ago } when we only looked at resin embedded samples on scope is that we have } had increasing number of users who look at nanoparticles. Unlike } biological material, these nano particles may not inhere to support } film as well therefore may “fly” away when beam hits them. This } problem can be worse when particles are not in a single layer on } support film. Also, users often do negative staining of these } nanoparticles in order to see the coating. I assume too much PTA or } UA dried onto the grids can contribute to the problem as well. } } We use our scope at 75 KV and usually use 10 or 20µm objective } apertures for adequate contrast. This means there are only 1 or 2 } apertures we can use in a standard objective aperture strip. Objective } aperture strips are very expensive (about $1300/ea). Obviously we } cannot use them as disposable items so often. } } My questions are: } } 1. Do you have many users looking at nanoparticles or negatively } stained materials on your scope, If yes, how long do you think I should } expect an objective aperture to last based on your experience. } 2. Is there anything else we should do to minimize contamination and } prolong the life of objective apertures. } } Thank you very much in advance. } } Hong } Emory SOM EM } } } } ==============================Original Headers============================== } 10, 20 -- From hyi-at-emory.edu Mon Oct 8 10:19:14 2007 } 10, 20 -- Received: from mr5.cc.emory.edu (mr5.cc.emory.edu [170.140.52.94]) } 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l98FJCiw032187 } 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 10:19:13 -0500 } 10, 20 -- Received: from emory.edu (emoryfloatdmz.cc.emory.edu [170.140.52.254]) } 10, 20 -- by mr5.cc.emory.edu (8.13.1/8.13.1) with ESMTP id l98FJ6H8008333 } 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 11:19:06 -0400 } 10, 20 -- Date: Mon, 8 Oct 2007 11:22:42 -0400 } 10, 20 -- Mime-Version: 1.0 (Apple Message framework v553) } 10, 20 -- Content-Type: text/plain; charset=WINDOWS-1252; format=flowed } 10, 20 -- Subject: Contamination on TEM } 10, 20 -- From: Hong Yi {hyi-at-emory.edu} } 10, 20 -- To: microscopy-at-microscopy.com } 10, 20 -- Message-Id: {50654733-75B2-11DC-AEFE-0003939EF2BC-at-emory.edu} } 10, 20 -- X-Mailer: Apple Mail (2.553) } 10, 20 -- X-emory.edu-MailScanner: Found to be clean } 10, 20 -- X-emory.edu-MailScanner-From: hyi-at-emory.edu } 10, 20 -- X-Spam-Status: No } 10, 20 -- Content-Transfer-Encoding: 8bit } 10, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l98FJCiw032187 } ==============================End of - Headers==============================
-- } {(((º} L L } {(((º} .·´¯`·.¸ {º)))} { ¸.·´¯`·.¸ } {(((º} ¸.·´¯¯¯¯¯¯¯
Dale A. Callaham Central Microscopy Facility The University of Massachusetts Amherst, MA 01003
==============================Original Headers============================== 11, 20 -- From dac-at-research.umass.edu Mon Oct 8 14:06:08 2007 11, 20 -- Received: from race5.oit.umass.edu (race5.oit.umass.edu [128.119.101.41]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l98J68lB022955 11, 20 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 14:06:08 -0500 11, 20 -- Received: from [192.168.1.2] (68-112-235-86.dhcp.oxfr.ma.charter.com [68.112.235.86]) 11, 20 -- (authenticated bits=0) 11, 20 -- by race5.oit.umass.edu (8.14.1/8.14.1) with ESMTP id l98J64P7014769 11, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 11, 20 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 15:06:05 -0400 11, 20 -- Message-ID: {470A8E1F.1090308-at-research.umass.edu} 11, 20 -- Date: Mon, 08 Oct 2007 15:07:59 -0500 11, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 11, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.6) Gecko/20070802 SeaMonkey/1.1.4 11, 20 -- MIME-Version: 1.0 11, 20 -- To: Microscopy List {microscopy-at-microscopy.com} 11, 20 -- Subject: Re: [Microscopy] Contamination on TEM 11, 20 -- References: {200710081524.l98FOAss008927-at-ns.microscopy.com} 11, 20 -- In-Reply-To: {200710081524.l98FOAss008927-at-ns.microscopy.com} 11, 20 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
In response to this, I would just say that the comparison isn't exactly correct. PCs use digital logic (think thresholds, and designed-in noise immunity....) and thus will be much less susceptible to certain levels of RF interference while much critical business-end circuitry on TEMs is by it's nature analog and IF the circuits picked up RF signals it could cause interference... The FCC does have standards and assuming those standards are met there may still be problems, and one of the suggested solutions IF interference is observed is move the offending equipment away, or reorient, etc..
I do not mean to suggest that the proliferation of wireless does actually cause problems.
Dale
beaurega-at-westol.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, } } Your EM column and the electronics are encased in metal parts and cabinets } that are grounded, I hope. A Faraday cage is a shield. Shielding is used } to contain or keep out electric fields and RF. So your EM is a nearly } perfect Faraday Shield, if properly grounded to the earth over a low } resistance path to a driven earth ground. } } The comments from John and Alan state that they don't see the problem. I } can see why. } } Here's a little paradox of sorts. How come a wireless RF transceiver right } next to your PC is not interferring with your PC but it does with your } grounded EM thirty feet away? The FCC and manufacturers are a few } shielding steps ahead of us. Even the inside surface of a plastic PC case } is metalized and grounded to reduce interference. } } JMO, } } Paul } Amateur Radio Station KC8O, extra class and commercial FCC license holder } for over thirty years. } } At 09:38 AM 10/8/07 -0500, you wrote: } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi Everyone, } } } } Please correct me if I am wrong, but doesn't the FCC regulate the } } frequencies that may be used by different devices? } } } } Is it possible to shield your instruments, perhaps with a Faraday cage? } } A partial cage made of wire mesh stapled onto movable partitions (grounded } } together) - like the partitions that divide office space into cubicles might } } even do the job. One would need to know the minimum mesh size of hardware } } cloth needed to block that frequency. } } } } Take care, Bob Carter } } } } Lopez Island, WA } } } } ----- Original Message ----- } } X-from: {oshel1pe-at-cmich.edu} } } To: {bob-at-rockisland.com} } } Sent: Friday, October 05, 2007 6:05 AM } } Subject: [Microscopy] Re: Wi-Fi and RF interference } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } ---------------------------------------------------------------------------- } } } micromavens, } } } } } } Please reply to list -- I'm in the same boat, except the IT people } } } here don't care if they cause interference. They just chose 2.4 GHz } } } for the WiFi, and if that interfers with cordless phones, it's your } } } fault. Instruments? Not even a consideration to them. } } } $*#^&$(#&(. } } } } } } Phil } } } } } } } ------------------------------------------------------------------------- } --- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } } To Subscribe/Unsubscribe -- } } } } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } ------------------------------------------------------------------------- } --- } } } } } } } } Good Morning: The Institution has decided to push wireless internet } } } } access into every corner of the campus. They also intend to boost the } } } } RF (cell phone) signal strength. I operate 3 TEMs, a LEO EFTEM, a } } } } cryo-Tecnai and an CM10. I worry that the TEMs will be affected by the } } } } signal frequency and strength from these new sources. I am being asked } } } } specific questions about which frequencies are harmful to our } } } } operation and am } } } } having trouble answering. If anyone has any experience or resources to } } } } help me communicate the scope of the problem it would be much } } } } appreciated. Thanks bob harris } } } } } } } } Guelph Regional Integrated Imaging Facility (GRIIF) } } } } Transmission Electron Microscope Facility } } } } Dept. of Molecular and Cell Biology } } } } New Science Complex, 488 Gordon St. } } } } University of Guelph } } } } Guelph Ontario, Canada, N1G 2W1 } } } } Phone: 519-824-4120 X 56409 } } } } Fax: 519-837-1802 } } } -- } } } Philip Oshel } } } Microscopy Facility Supervisor } } } Biology Department } } } 024C Brooks Hall } } } Central Michigan University } } } Mt. Pleasant, MI 48859 } } } } } } ==============================Original } } } Headers============================== } } } 4, 22 -- From oshel1pe-at-cmich.edu Fri Oct 5 08:05:00 2007 } } } 4, 22 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) } } } 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } } l95D50BS012416 } } } 4, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 08:05:00 -0500 } } } 4, 22 -- Received: from egatea.central.cmich.local ([141.209.15.74]) } } } 4, 22 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id l95DPb9N012007 } } } 4, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 09:25:38 -0400 } } } 4, 22 -- Received: from [141.209.160.249] ([141.209.160.249]) by } } } egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); } } } 4, 22 -- Fri, 5 Oct 2007 09:04:58 -0400 } } } 4, 22 -- Mime-Version: 1.0 } } } 4, 22 -- Message-Id: {f06240806c32be66fb196-at-[141.209.160.249]} } } } 4, 22 -- In-Reply-To: {200710051253.l95CrvJt006754-at-ns.microscopy.com} } } } 4, 22 -- References: {200710051253.l95CrvJt006754-at-ns.microscopy.com} } } } 4, 22 -- Date: Fri, 5 Oct 2007 09:04:55 -0400 } } } 4, 22 -- To: Microscopy-at-microscopy.com } } } 4, 22 -- From: Philip Oshel {oshel1pe-at-cmich.edu} } } } 4, 22 -- Subject: Re: [Microscopy] Wi-Fi and RF interference } } } 4, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } } } 4, 22 -- X-OriginalArrivalTime: 05 Oct 2007 13:04:58.0814 (UTC) } } } FILETIME=[55316DE0:01C80750] } } } 4, 22 -- X-CanItPRO-Stream: default } } } 4, 22 -- X-Spam-Score: -3.2 () CELL_PHONE_IMPROVE,L_EXCH_MF } } } 4, 22 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 } } } ==============================End of - } } } Headers============================== } } } } } } } } } -- } } } No virus found in this incoming message. } } } Checked by AVG Free Edition. } } } Version: 7.5.488 / Virus Database: 269.14.0/1049 - Release Date: 10/4/2007 } } } 8:59 AM } } } } } } } } } } ==============================Original Headers============================== } } 9, 31 -- From bob-at-rockisland.com Mon Oct 8 09:37:36 2007 } } 9, 31 -- Received: from mars.rockisland.com (lists.rockisland.com } [64.119.0.12]) } } 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } l98EbZSu018685 } } 9, 31 -- for {microscopy-at-microscopy.com} ; 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} } 9, 31 -- format=flowed; } } 9, 31 -- charset="Windows-1252"; } } 9, 31 -- reply-type=original } } 9, 31 -- Content-Transfer-Encoding: 7bit } } 9, 31 -- X-Priority: 3 } } 9, 31 -- X-MSMail-Priority: Normal } } 9, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 } } 9, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 } } ==============================End of - Headers============================== } } } } } } } } ==============================Original Headers============================== } 7, 26 -- From beaurega-at-westol.com Mon Oct 8 13:40:09 2007 } 7, 26 -- Received: from smtp-gateway-5.winbeam.com (smtp-gateway-5.winbeam.com [64.84.97.70]) } 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l98Ie7Li010541 } 7, 26 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 13:40:09 -0500 } 7, 26 -- X-Winbeam-MailScanner-Watermark: 1192473512.59247-at-4MFKYxQxXUEMEqqOknAIeg } 7, 26 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) } 7, 26 -- by smtp-gateway-5.winbeam.com (8.13.2/8.12.8) with SMTP id l98IcTd2013027 } 7, 26 -- for {microscopy-at-microscopy.com} ; 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-- } {(((º} L L } {(((º} .·´¯`·.¸ {º)))} { ¸.·´¯`·.¸ } {(((º} ¸.·´¯¯¯¯¯¯¯
Dale A. Callaham Central Microscopy Facility The University of Massachusetts Amherst, MA 01003
==============================Original Headers============================== 8, 20 -- From dac-at-research.umass.edu Mon Oct 8 14:24:26 2007 8, 20 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l98JOQlo002824 8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 14:24:26 -0500 8, 20 -- Received: from [192.168.1.2] (68-112-235-86.dhcp.oxfr.ma.charter.com [68.112.235.86]) 8, 20 -- (authenticated bits=0) 8, 20 -- by race1.oit.umass.edu (8.14.1/8.14.1) with ESMTP id l98JOP6h002522 8, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 20 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 15:24:25 -0400 8, 20 -- Message-ID: {470A926A.50102-at-research.umass.edu} 8, 20 -- Date: Mon, 08 Oct 2007 15:26:18 -0500 8, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 8, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.6) Gecko/20070802 SeaMonkey/1.1.4 8, 20 -- MIME-Version: 1.0 8, 20 -- To: Microscopy List {microscopy-at-microscopy.com} 8, 20 -- Subject: Re: [Microscopy] Re: Wi-Fi and RF interference 8, 20 -- References: {200710081845.l98Ijcav019696-at-ns.microscopy.com} 8, 20 -- In-Reply-To: {200710081845.l98Ijcav019696-at-ns.microscopy.com} 8, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 20 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jean-ross-at-uiowa.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jean-ross-at-uiowa.edu Name: Jean Ross
Organization: Central Microscopy Research Facility, University of Iowa
Title-Subject: [Filtered] JEOL 1230 filament life
Question: Hi everyone,
I would like to get some feedback from others on this list who have a JEOL 1230 as to how many hours that you typically get from your filaments. We are trying to see if we are in the same ballpark as everyone else and we would like to better anticipate when we will need to change filaments. Thanks in advance.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both klangwor-at-uoregon.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: klangwor-at-uoregon.edu Name: Kurt Langworthy
Organization: University of Oregon
Title-Subject: [Filtered] small vacuum cleaner
Question: Does anyone know of a manufacture that produces a small vacuum cleaner tool to use on cleaning SEM parts, and to remove loose particles from samples?
We look at over 3000 negative stained samples for viral diagnostics every year. We have to scan at --|20,000x, and we print at 200,000x. In my experience, both the moly and platinum apertures have very limited time of use between cleaning when you try to use them for any high magnification/high resolution work. This is especially true where the samples are negatively stained. With an average workload of 60 samples/week, we would be cleaning the apertures every 1-2 weeks. In short, I find them unsuitable.
I would never work a microscope without a gold foil objective aperture. The high energy of the beam burns off contamination as it gathers on the aperture. Periodically (every 2-3 months) I focus the beam and run it around the edge of the aperture to clean it up. Don't know if that helps, but I do it any way. I keep both a 20 and 30 micron gold foil aperture in the scope, and usually use the 20. I replace the 20 micron aperture maybe once a year - it is usually more like every 18 months. I use the 30 when the 20 is getting a bit dirty and we haven't got any spare 20 apertures in stock.
The major problem I have is keeping the specimen grid holder clean. We clean that about every 2 weeks. Every thing works well between annual cleanings with these protocols.
Paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Pager:204-931-9354 Cell:204-781-1502 Fax:204-789-3926
==============================Original Headers============================== 10, 19 -- From paul_hazelton-at-umanitoba.ca Mon Oct 8 18:03:49 2007 10, 19 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l98N3mhZ011551 10, 19 -- for {microscopy-at-microscopy.com} ; Mon, 8 Oct 2007 18:03:48 -0500 10, 19 -- Received: from [192.168.100.101] (wnpgmb01dc2-25-152.dynamic.mts.net [142.161.25.152]) 10, 19 -- (authenticated bits=0) 10, 19 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id l98N3idT005375 10, 19 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NO); 10, 19 -- Mon, 8 Oct 2007 18:03:46 -0500 (CDT) 10, 19 -- Message-ID: {470AB753.8080809-at-umanitoba.ca} 10, 19 -- Date: Mon, 08 Oct 2007 18:03:47 -0500 10, 19 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} 10, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 10, 19 -- X-Accept-Language: en-us, en 10, 19 -- MIME-Version: 1.0 10, 19 -- To: Hong Yi {hyi-at-emory.edu} , Microscopy Listserver {microscopy-at-microscopy.com} 10, 19 -- Subject: Contamination on TEM 10, 19 -- Content-Type: text/plain; charset=us-ascii; format=flowed 10, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I fully understand your problem and respect the ideas passed on by others, but have you tried running at one kV step higher. A higher kV will not "cook" your sample so quickly and will be far less sensitive to contamination on the apertures. I have many clients working in the biological field, particularly with virus work, who routinely use 100/120/125kV, give it a try?
Two positive steps with just one change!
Steve Chapman Senior Consultant Protrain For electron microscopy consultancy and training world wide Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967 Web www.emcourses.com
----- Original Message ----- X-from: {paul_hazelton-at-umanitoba.ca} To: {protrain-at-emcourses.com} Sent: Tuesday, October 09, 2007 12:04 AM
This might be interesting for the people who use the Southbay single jet electro polisher. I carry with me a Motorola i760 cell phone using the Nextel/Sprint provider. I noticed that about 2 seconds before I received an incoming call that the auto shutoff on the control unit would activate shutting off the system. Typically this will happen when the sensor detects a perforation in the sample. I confirmed that the sample was not perforated and that the incoming call affected the electronics in some way. Nestor and I did some simple experiments with this and it seems the phone does not affect anything as long as it was more than 2 feet away from the control unit. Also, this does not happen with all phones. Nestor has an LG C2000 cell phone using AT&T and it did not trigger the auto shutoff.
Jon
Jon M. Hiller Electron Microscopy Center Argonne National Laboratory 9700 South Cass Avenue Building 212, Room A257 630-252-7904
dac-at-research.umass.edu wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } In response to this, I would just say that the comparison isn't exactly } correct. PCs use digital logic (think thresholds, and designed-in noise } immunity....) and thus will be much less susceptible to certain levels } of RF interference while much critical business-end circuitry on TEMs is } by it's nature analog and IF the circuits picked up RF signals it could } cause interference... The FCC does have standards and assuming those } standards are met there may still be problems, and one of the suggested } solutions IF interference is observed is move the offending equipment } away, or reorient, etc.. } } I do not mean to suggest that the proliferation of wireless does } actually cause problems. } } Dale } } } } beaurega-at-westol.com wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi, } } } } Your EM column and the electronics are encased in metal parts and cabinets } } that are grounded, I hope. A Faraday cage is a shield. Shielding is used } } to contain or keep out electric fields and RF. So your EM is a nearly } } perfect Faraday Shield, if properly grounded to the earth over a low } } resistance path to a driven earth ground. } } } } The comments from John and Alan state that they don't see the problem. I } } can see why. } } } } Here's a little paradox of sorts. How come a wireless RF transceiver right } } next to your PC is not interferring with your PC but it does with your } } grounded EM thirty feet away? The FCC and manufacturers are a few } } shielding steps ahead of us. Even the inside surface of a plastic PC case } } is metalized and grounded to reduce interference. } } } } JMO, } } } } Paul } } Amateur Radio Station KC8O, extra class and commercial FCC license holder } } for over thirty years. } } } } At 09:38 AM 10/8/07 -0500, you wrote: } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Hi Everyone, } } } } } } Please correct me if I am wrong, but doesn't the FCC regulate the } } } frequencies that may be used by different devices? } } } } } } Is it possible to shield your instruments, perhaps with a Faraday cage? } } } A partial cage made of wire mesh stapled onto movable partitions (grounded } } } together) - like the partitions that divide office space into cubicles might } } } even do the job. One would need to know the minimum mesh size of hardware } } } cloth needed to block that frequency. } } } } } } Take care, Bob Carter } } } } } } Lopez Island, WA } } } } } } ----- Original Message ----- } } } X-from: {oshel1pe-at-cmich.edu} } } } To: {bob-at-rockisland.com} } } } Sent: Friday, October 05, 2007 6:05 AM } } } Subject: [Microscopy] Re: Wi-Fi and RF interference } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } } To Subscribe/Unsubscribe -- } } } } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------------- } } } } micromavens, } } } } } } } } Please reply to list -- I'm in the same boat, except the IT people } } } } here don't care if they cause interference. They just chose 2.4 GHz } } } } for the WiFi, and if that interfers with cordless phones, it's your } } } } fault. Instruments? Not even a consideration to them. } } } } $*#^&$(#&(. } } } } } } } } Phil } } } } } } } } } ------------------------------------------------------------------------- } } --- } } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } } } To Subscribe/Unsubscribe -- } } } } } http://www.microscopy.com/MicroscopyListserver } } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ------------------------------------------------------------------------- } } --- } } } } } Good Morning: The Institution has decided to push wireless internet } } } } } access into every corner of the campus. They also intend to boost the } } } } } RF (cell phone) signal strength. I operate 3 TEMs, a LEO EFTEM, a } } } } } cryo-Tecnai and an CM10. I worry that the TEMs will be affected by the } } } } } signal frequency and strength from these new sources. I am being asked } } } } } specific questions about which frequencies are harmful to our } } } } } operation and am } } } } } having trouble answering. If anyone has any experience or resources to } } } } } help me communicate the scope of the problem it would be much } } } } } appreciated. Thanks bob harris } } } } } } } } } } Guelph Regional Integrated Imaging Facility (GRIIF) } } } } } Transmission Electron Microscope Facility } } } } } Dept. of Molecular and Cell Biology } } } } } New Science Complex, 488 Gordon St. } } } } } University of Guelph } } } } } Guelph Ontario, Canada, N1G 2W1 } } } } } Phone: 519-824-4120 X 56409 } } } } } Fax: 519-837-1802 } } } } -- } } } } Philip Oshel } } } } Microscopy Facility Supervisor } } } } Biology Department } } } } 024C Brooks Hall } } } } Central Michigan University } } } } Mt. Pleasant, MI 48859 } } } } } } } } ==============================Original } } } } Headers============================== } } } } 4, 22 -- From oshel1pe-at-cmich.edu Fri Oct 5 08:05:00 2007 } } } } 4, 22 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) } } } } 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } } } l95D50BS012416 } } } } 4, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 08:05:00 -0500 } } } } 4, 22 -- Received: from egatea.central.cmich.local ([141.209.15.74]) } } } } 4, 22 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id l95DPb9N012007 } } } } 4, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Oct 2007 09:25:38 -0400 } } } } 4, 22 -- Received: from [141.209.160.249] ([141.209.160.249]) by } } } } egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); } } } } 4, 22 -- Fri, 5 Oct 2007 09:04:58 -0400 } } } } 4, 22 -- Mime-Version: 1.0 } } } } 4, 22 -- Message-Id: {f06240806c32be66fb196-at-[141.209.160.249]} } } } } 4, 22 -- In-Reply-To: {200710051253.l95CrvJt006754-at-ns.microscopy.com} } } } } 4, 22 -- References: {200710051253.l95CrvJt006754-at-ns.microscopy.com} } } } } 4, 22 -- Date: Fri, 5 Oct 2007 09:04:55 -0400 } } } } 4, 22 -- To: Microscopy-at-microscopy.com } } } } 4, 22 -- From: Philip Oshel {oshel1pe-at-cmich.edu} } } } } 4, 22 -- Subject: Re: [Microscopy] Wi-Fi and RF interference } } } } 4, 22 -- Content-Type: text/plain; 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==============================Original Headers============================== 10, 26 -- From hiller-at-anl.gov Tue Oct 9 12:08:58 2007 10, 26 -- Received: from mailrelay.anl.gov (mailrelay.anl.gov [130.202.101.22]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l99H8vw7011765 10, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Oct 2007 12:08:57 -0500 10, 26 -- Received: from mailrelay.anl.gov (localhost [127.0.0.1]) 10, 26 -- by localhost.ctd.anl.gov (Postfix) with ESMTP id 26FB35F0C01; 10, 26 -- Tue, 9 Oct 2007 12:08:53 -0500 (CDT) 10, 26 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on frigga.ctd.anl.gov 10, 26 -- X-Spam-Status: No, score=0.0 required=5.7 tests=none autolearn=disabled 10, 26 -- version=3.1.8 10, 26 -- X-Spam-Level: 10, 26 -- Received: from [146.139.150.121] (hiller-c225.msd.anl.gov [146.139.150.121]) 10, 26 -- by mailrelay.anl.gov (Postfix) with ESMTP id E6B4C5F0C02 10, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Oct 2007 12:08:52 -0500 (CDT) 10, 26 -- Message-ID: {470BB5A4.3080904-at-anl.gov} 10, 26 -- Date: Tue, 09 Oct 2007 12:08:52 -0500 10, 26 -- From: "Jon M. Hiller" {hiller-at-anl.gov} 10, 26 -- Organization: Argonne National Laboratory 10, 26 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 10, 26 -- MIME-Version: 1.0 10, 26 -- To: Microscopy-at-microscopy.com 10, 26 -- Subject: Re: [Microscopy] Wi-Fi and RF interference 10, 26 -- References: {200710081925.l98JP08w003828-at-ns.microscopy.com} 10, 26 -- In-Reply-To: {200710081925.l98JP08w003828-at-ns.microscopy.com} 10, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (hoelyas-at-yahoo.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, October 9, 2007 at 15:30:28 ---------------------------------------------------------------------------
Email: hoelyas-at-yahoo.com Name: H.Elyas
Education: Graduate College
Location: City, State, Country
Question: Hi All I need sevice manual or if not, schematic diagram of "Zeiss EM900" TEM. to repair my instrument please someone guid me what I have to do. Best Regards, Elyas
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both scott.coutts-at-med.monash.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: scott.coutts-at-med.monash.edu.au Name: Scott Coutts
Can anyone make a recommendation for a gold label for immunolabelling? We don't want to conjugate our own gold. We'll be labelling whole cells and sections in LR white. The primary is polyclonal rabbit. Other than the species specificity issues, is there a general consensus on whether IgG or protein A is better for labelling? What about brands?
The University of Minnesota Veterinary Diagnostic Laboratory has an Assistant Scientist position open in the Electron Microscopy Laboratory. This position is a diagnostic, research, and supervisory role, and involves both negative staining and resin-embedded tissue TEM.
Anyone interested in more information may contact me directly (email is best).
Jan Shivers Section Head - Electron Microscopy/Immunohistochemistry/Histology Veterinary Diagnostic Laboratory College of Veterinary Medicine St. Paul, MN 55108 612-624-7297 shive003-at-umn.edu
==============================Original Headers============================== 4, 23 -- From shive003-at-umn.edu Wed Oct 10 08:19:38 2007 4, 23 -- Received: from mta-w2.tc.umn.edu (mta-w2.tc.umn.edu [134.84.119.6]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9ADJcrE008765 4, 23 -- for {Microscopy-at-Microscopy.Com} ; Wed, 10 Oct 2007 08:19:38 -0500 4, 23 -- Received: from vdltemp1 (x84-6-161-dhcp.cvm.umn.edu [134.84.6.161]) 4, 23 -- by mta-w2.tc.umn.edu (UMN smtpd) with ESMTP 4, 23 -- for {Microscopy-at-Microscopy.Com} ; Wed, 10 Oct 2007 08:19:37 -0500 (CDT) 4, 23 -- X-Umn-Remote-Mta: [N] x84-6-161-dhcp.cvm.umn.edu [134.84.6.161] #+LO+TS+AU+HN 4, 23 -- Message-ID: {002001c80b40$35798cf0$a1065486-at-auxs.umn.edu} 4, 23 -- From: "Jan Shivers" {shive003-at-umn.edu} 4, 23 -- To: {Microscopy-at-Microscopy.Com} 4, 23 -- Subject: job opening 4, 23 -- Date: Wed, 10 Oct 2007 08:19:37 -0500 4, 23 -- MIME-Version: 1.0 4, 23 -- Content-Type: text/plain; 4, 23 -- format=flowed; 4, 23 -- charset="iso-8859-1"; 4, 23 -- reply-type=original 4, 23 -- Content-Transfer-Encoding: 7bit 4, 23 -- X-Priority: 3 4, 23 -- X-MSMail-Priority: Normal 4, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 4, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3138 ==============================End of - Headers==============================
For species specific gold conjugated secondaries against rabbit primaries we have used both BBI international available through Ted Pella and Aurion avaliable through EMS (Electron Microscopy Sciences) with excellent results in sizes from ultrasmall (Aurion, requiring enhancement) through 40nm. Labelling frequency tends to go down as the size of gold increases. We have little experience with protein A. Two things that I have noticed that have been helpful. 1) Use PBS rather than TBS for a buffer as the TBS incompatibility with the antibody buffer will tend to cause clustering of the antibodies not noticable at the light level. 2) We use only BSA not species specific serum like goat serum, for blocking. The goat serum tends to be too good and the signal is reduced by a factor of ten for most of our antibodies.
Robert Underwood University of Washington Dermatology Research Center Seattle, WA
---------- Forwarded message ----------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both scott.coutts-at-med.monash.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: scott.coutts-at-med.monash.edu.au Name: Scott Coutts
Can anyone make a recommendation for a gold label for immunolabelling? We don't want to conjugate our own gold. We'll be labelling whole cells and sections in LR white. The primary is polyclonal rabbit. Other than the species specificity issues, is there a general consensus on whether IgG or protein A is better for labelling? What about brands?
A researcher has brought an epoxy-embedded sample that had been treated with 20nm zinc oxide particles. I sectioned the material and I see the electron-dense particles and clusters dispersed in un-stained sections by conventional TEM. The researcher wants to know if these dots are indeed Zn. I have been reading websites and advertisments all morning and I just can't easily find anything that states the limits of detectivity (spatial) for elemental mapping/detection this type of sample. I would greatly appreciate any information that I can pass on to this researcher regarding how to proceed, what type of instrumentation to be looking for, etc. Our facility has no analytical instrumentation on any of our EMs and I haven't devoted much time keeping up with the details of instruments we don't have for work we don't provide.
In a nutshell: Botanical material (root) with 20nm zinc oxide particles/clusters, fixed and embedded in epoxy resin; particles may be inside or outside of the root cells. Is it possible to get images and elemental analysis/maps from these tiny particles?
Also, if there is an analytical service that can work with these samples, please contact me.
Thanks,
Dale Callaham
==============================Original Headers============================== 6, 20 -- From dac-at-research.umass.edu Wed Oct 10 11:01:28 2007 6, 20 -- Received: from race2.oit.umass.edu (race2.oit.umass.edu [128.119.101.38]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9AG1SbU000630 6, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Oct 2007 11:01:28 -0500 6, 20 -- Received: from [172.30.55.164] (eutopia.bio.umass.edu [128.119.55.30]) 6, 20 -- (authenticated bits=0) 6, 20 -- by race2.oit.umass.edu (8.14.1/8.14.1) with ESMTP id l9AG1RmV005161 6, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 6, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Oct 2007 12:01:27 -0400 6, 20 -- Message-ID: {470D0579.2000607-at-research.umass.edu} 6, 20 -- Date: Wed, 10 Oct 2007 12:01:45 -0500 6, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 6, 20 -- Reply-To: dac-at-research.umass.edu 6, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.6) Gecko/20070802 SeaMonkey/1.1.4 6, 20 -- MIME-Version: 1.0 6, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 20 -- Subject: Practical expectations in elemental mapping? 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- X-Whitelist: TRUE ==============================End of - Headers==============================
Electron Microscopy Laboratory of the Physics Department, Aristotle University of Thessaloniki, Greece, is organizing the winter school
"Growth and characterization of advanced materials focused on structural characterization"
from January 14th through 18th, 2008.
The school is addressed to PhD and postgraduate students. Apart from the lectures, a hands-on training on specimen preparation techniques will also be offered. For more information, please visit: http://pam1.physics.auth.gr or contact the school coordinator, Prof. E. K. Polychroniadis, at pam1-at-physics.auth.gr.
Best regards, E.K. Polychroniadis
E.K. Polychroniadis Department of Physics Aristotle University of Thessaloniki Thessaloniki 54124, Greece Tel.: +30.2310.998163 Fax: +30.2310.998241 e-mail: polychr-at-auth.gr
==============================Original Headers============================== 14, 25 -- From polychr-at-auth.gr Wed Oct 10 14:46:58 2007 14, 25 -- Received: from hyperion.ccf.auth.gr (hyperion.ccf.auth.gr [155.207.1.42]) 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9AJkun4025844 14, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Oct 2007 14:46:57 -0500 14, 25 -- Received: from auth.gr (electra.ccf.auth.gr [155.207.1.59]) 14, 25 -- by hyperion.ccf.auth.gr (8.14.1/8.14.1) with ESMTP id l9AJkutM011533 14, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Oct 2007 22:46:56 +0300 14, 25 -- Received: from kerasia.physics.auth.gr (kerasia.physics.auth.gr 14, 25 -- [155.207.11.93]) by webmail.auth.gr (Horde MIME library) with HTTP; Wed, 10 14, 25 -- Oct 2007 22:46:56 +0300 14, 25 -- Message-ID: {20071010224656.xauaugbuwwcw4wgk-at-webmail.auth.gr} 14, 25 -- Date: Wed, 10 Oct 2007 22:46:56 +0300 14, 25 -- From: Efstathios Polychroniadis {polychr-at-auth.gr} 14, 25 -- To: Microscopy-at-microscopy.com 14, 25 -- Subject: Winter School 14, 25 -- MIME-Version: 1.0 14, 25 -- Content-Type: text/plain; 14, 25 -- charset=ISO-8859-7; 14, 25 -- DelSp="Yes"; 14, 25 -- format="flowed" 14, 25 -- Content-Disposition: inline 14, 25 -- Content-Transfer-Encoding: 7bit 14, 25 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.4-cvs) 14, 25 -- X-Virus-Scanned: ClamAV 0.91.2/4523/Wed Oct 10 21:30:26 2007 on cronus.ccf.auth.gr 14, 25 -- X-Virus-Status: Clean ==============================End of - Headers==============================
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I would have said that this was relatively straightforward on any 'current' analytical TEM fitted with EDS.
Even without EDS, you can probably use electron diffraction to confirm Zinc Oxide by d-spacings in ring diffraction patterns and dark field imaging to show the distribution of particles.
In either case, you aren't easily going to be able to do much in the way of quantification.
Best regards, -- Larry Stoter
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==============================Original Headers============================== 6, 15 -- From larry-at-cymru666.plus.com Wed Oct 10 15:53:41 2007 6, 15 -- Received: from pih-relay08.plus.net (pih-relay08.plus.net [212.159.14.134]) 6, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9AKrehP007698 6, 15 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 10 Oct 2007 15:53:40 -0500 6, 15 -- Received: from [87.115.1.173] (helo=[192.168.1.2]) 6, 15 -- by pih-relay08.plus.net with esmtp (Exim) id 1IfiYl-00018e-8E; Wed, 10 Oct 2007 21:53:39 +0100 6, 15 -- Mime-Version: 1.0 6, 15 -- Message-Id: {p06240800c332e5e65ed0-at-[192.168.1.2]} 6, 15 -- In-Reply-To: {200710101607.l9AG7PLo017324-at-ns.microscopy.com} 6, 15 -- References: {200710101607.l9AG7PLo017324-at-ns.microscopy.com} 6, 15 -- Date: Wed, 10 Oct 2007 21:53:26 +0100 6, 15 -- To: dac-at-research.umass.edu, Microscopy-at-MSA.Microscopy.Com 6, 15 -- From: Larry Stoter {larry-at-cymru666.plus.com} 6, 15 -- Subject: Re: [Microscopy] Practical expectations in elemental mapping? 6, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Ok. This question is a bit personal- I just found out that our puppy has hook worms. Me being, well, me, I want to get as much info as possible on these little things. Does anybody know how to capture them so I can check them out under a light microscope? I've got some protocols for staining and prep, but no idea how to actually get them.
I'd like to do an SEM scan of one of them as well, but I want to check them out in light first.
I actually have a group of students who want to test a variety of preventative measures on these worms, since we apparently have a species that is immune to the Revolution medicine that my dog is on.
Sorry if this one seems a bit too personal and not technical, but I figure someone out there on this list has had some experience with getting these things.
--Justin A. Kraft
==============================Original Headers============================== 5, 27 -- From kraftpiano-at-gmail.com Wed Oct 10 21:00:54 2007 5, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.188]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9B20s8k028381 5, 27 -- for {microscopy-at-microscopy.com} ; Wed, 10 Oct 2007 21:00:54 -0500 5, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so359133rvb 5, 27 -- for {microscopy-at-microscopy.com} ; Wed, 10 Oct 2007 19:00:54 -0700 (PDT) 5, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 27 -- d=gmail.com; s=beta; 5, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- bh=nfw7o3U7/tYrQeNfnnm2/NbdkhkM1pTmEjtjHs3HMhY=; 5, 27 -- b=tptCWR34gheEUBD0R3033KnWAQKi/Saj+HGryjNwLwtdOKHNvJ14vCokc57VgI4WxWODnNtB7NZ08kv9LU9HfXkIHscZyPt/4LDYszbqwae/FSO8dUMCD6dSNwcbtz+n4bZfbiy4jONeFHaJSjRb4xput6PkL7P0XrQFSmFVorM= 5, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 27 -- d=gmail.com; s=beta; 5, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- b=E10/jt+fpjfimSEtiSgZ/gjMFTONtMM8RG8o0HkcbOocSnqYAXtA5W3nUXprb3iQPtG1JfiavIYkH+8dGPcPtJwOPDzjVFisuPaEN2tXkqWJpvUhY/MuqCJQ7Bc0VrZhTRv7ErNtKYvuetp/4qV1E96OmvaUhORMWPJolGfxcoM= 5, 27 -- Received: by 10.140.193.16 with SMTP id q16mr676928rvf.1192068054056; 5, 27 -- Wed, 10 Oct 2007 19:00:54 -0700 (PDT) 5, 27 -- Received: by 10.141.172.5 with HTTP; Wed, 10 Oct 2007 19:00:54 -0700 (PDT) 5, 27 -- Message-ID: {25e2b0d20710101900q70aed2byde03fd0405038260-at-mail.gmail.com} 5, 27 -- Date: Wed, 10 Oct 2007 22:00:54 -0400 5, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 5, 27 -- To: microscopy-at-microscopy.com 5, 27 -- Subject: Specimen capture question. 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Type: text/plain; charset=ISO-8859-1 5, 27 -- Content-Transfer-Encoding: 7bit 5, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Dear mail listers, I work in the Centre ENEA in Brindisi with a Transmission Electron Microscope TECNAI G2 F30 with a super Twin objective lens.. In these days we have a problem with the cooling system of the instrument. It consists of two chillers: ZEM 1000 S, which is the principal cooling system, and a ZEM 300 SW, a secondary chiller (useful for the objective lens) which works in connection with the first one. The chiller ZEM 300 SW sometimes stops, the temperature goes up with the consequent switching off of the objective lens; when it works again (we have to change the setting of the thermostatic valve), the temperature decreases very fast, but goes down too much (until 6 °C). In order to cause an increase of its temperature we have to modify the water flow of the objecive lens in the valve unit of the TEM. We suspect. that there is some problem with the thermostatic valve, but we have never received the instruction manual of the chiller Zephyr ZEM 300 SW. We need as soon as possible to solve this problem because it is not possible to use the microscope and because the continuos changing of the water flow of the objctive lens in the valve unit seems to cause also great variations of the water flow in the other lenses of the column and in the diffusion pump. Have somebody else noticed something like this problem? Can someone help us? We need also to find the user manual of the chiller ZEM 300 SW (Zephyr) or of a similar water chiller, could please someone help me to find it ? Attention: the chiller ZEM 300 SW works very different from the chiller Zephyr ZEM 1000 S: for this one we already have the manual! Any advice is welcome!! Thanks in advance Marilena Re
Marilena Re ENEA - Materials and Technology Composite and Nanostructured Materials Section C.R. Brindisi S.S. 7 Appia - km 713,700 72100 - Brindisi Italy marilena.re-at-brindisi.enea.it tel +39-(0)831 201444 fax +39-(0)831 201581
==============================Original Headers============================== 3, 23 -- From marilena.re-at-brindisi.enea.it Thu Oct 11 03:55:08 2007 3, 23 -- Received: from brindisi.enea.it (mail.brindisi.enea.it [192.107.88.201]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9B8t3BV019226 3, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Oct 2007 03:55:08 -0500 3, 23 -- Received: from [192.168.173.193] (HELO re3) 3, 23 -- by brindisi.enea.it (CommuniGate Pro SMTP 5.1.12) 3, 23 -- with SMTP id 5040926; Thu, 11 Oct 2007 10:50:14 +0200 3, 23 -- Message-ID: {002501c80be4$58d60850$c1ada8c0-at-re3} 3, 23 -- From: "Marilena Re" {marilena.re-at-brindisi.enea.it} 3, 23 -- To: {Microscopy-at-microscopy.com} 3, 23 -- Subject: Problem with a chiller ZEM 300 SW of a TEM TECNAI G2 F30 3, 23 -- Date: Thu, 11 Oct 2007 10:54:34 +0200 3, 23 -- MIME-Version: 1.0 3, 23 -- Content-Type: text/plain; 3, 23 -- format=flowed; 3, 23 -- charset="iso-8859-1"; 3, 23 -- reply-type=original 3, 23 -- Content-Transfer-Encoding: 8bit 3, 23 -- X-Priority: 3 3, 23 -- X-MSMail-Priority: Normal 3, 23 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 3, 23 -- Disposition-Notification-To: "Marilena Re" {marilena.re-at-brindisi.enea.it} 3, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
The Midwest Microscopy and Microanalysis Society will hold its next meeting, "Imaging at the Crossroads of Biological and Physical Sciences", on Friday, October 19, 2007, at the University of Wisconsin at Madison. The program and registration information can be found on our website:
www.midwestmicroscopy.org
We look forward to seeing you in Madison.
Elaine Schumacher President, Midwest Microscopy and Microanalysis Society
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 9, 27 -- From eschumacher-at-mccrone.com Thu Oct 11 08:07:53 2007 9, 27 -- Received: from pgp.mccrone.com (McCrone-Associates-1051626.cust-rtr.ameritech.net [68.23.63.122] (may be forged)) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9BD7rBU007615 9, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 11 Oct 2007 08:07:53 -0500 9, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 9, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 080211A800B 9, 27 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 11 Oct 2007 08:07:54 -0500 (CDT) 9, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 9, 27 -- by pgp.mccrone.com (PGP Universal service); 9, 27 -- Thu, 11 Oct 2007 08:07:54 -0500 9, 27 -- X-PGP-Universal: processed 9, 27 -- Content-class: urn:content-classes:message 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Type: text/plain; 9, 27 -- charset="US-ASCII" 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 27 -- Subject: Meeting Announcement: Midwest Microscopy and Microanalysis Society 9, 27 -- Date: Thu, 11 Oct 2007 08:07:47 -0500 9, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150D9E6-at-MCCRONEMSG.tmg.mccrone.com} 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- Thread-Topic: Meeting Announcement: Midwest Microscopy and Microanalysis Society 9, 27 -- Thread-Index: AcgMB7f+iciSRMCGRICaeI31rs/C/Q== 9, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 9, 27 -- To: {Microscopy-at-MSA.Microscopy.Com} 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l9BD7rBU007615 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both csd8-at-cornell.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: csd8-at-cornell.edu Name: Carole Daugherty
Organization: Cornell University
Title-Subject: [Filtered] xray analysis equipment on TEM
Question: I am searching for a group that has a functioning xray analysis system on a TEM. I am at Cornell in Ithaca, NY so I would like to find a system close to Ithaca. I have sections ready for viewing and analysis.
We have just had a Cambridge S200 Scanning electron microscope donated to us by Motorola. (Actually 2 of them, one for spare parts). I am having some issues getting it running. The relays inside the high voltage power supply are not getting flipped, so the CRT does not light, and the ion pump etc. is not getting power. Any ideas where to start? I think I have connected all the interlocks. Could this possibly be a vacuum leak problem? The roughing pump and turbo pump both work but the column does not drop down beyond 10-6 Torr.
-Dr. Mike Brown Science Dept Chair AAEC_PV
==============================Original Headers============================== 6, 17 -- From mbrown-at-aaechighschools.com Fri Oct 12 15:51:40 2007 6, 17 -- Received: from smtpoutwbe08.prod.mesa1.secureserver.net (smtpoutwbe08.prod.mesa1.secureserver.net [208.109.78.210]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l9CKpeLp032651 6, 17 -- for {Microscopy-at-Microscopy.Com} ; Fri, 12 Oct 2007 15:51:40 -0500 6, 17 -- Received: (qmail 23565 invoked from network); 12 Oct 2007 20:51:39 -0000 6, 17 -- Received: from unknown (HELO gem-wbe29.prod.mesa1.secureserver.net) (64.202.189.163) 6, 17 -- by smtpoutwbe08.prod.mesa1.secureserver.net with SMTP; 12 Oct 2007 20:51:39 -0000 6, 17 -- Received: (qmail 31954 invoked by uid 99); 12 Oct 2007 20:51:39 -0000 6, 17 -- Date: Fri, 12 Oct 2007 13:51:39 -0700 6, 17 -- From: mbrown-at-aaechighschools.com 6, 17 -- Subject: High voltage issues with Cambridge S200 6, 17 -- To: Microscopy-at-Microscopy.Com 6, 17 -- Message-ID: {20071012135139.889a5134facffa13190a02200f773d01.b2c1927c01.wbe-at-email.secureserver.net} 6, 17 -- MIME-Version: 1.0 6, 17 -- Content-Type: TEXT/plain; CHARSET=US-ASCII 6, 17 -- User-Agent: Web-Based Email 4.11.6 6, 17 -- X-Originating-IP: 72.223.68.165 ==============================End of - Headers==============================
For something to start with, try 10 nm Protein A-gold. Protein A will give you more precise localization compared to IgG-gold, because only one PA can bind to the primary Ab molecule. Thus, each gold particle you see will be pointing to a primary Ab. You really have to try one day and compare both in parallel - you won't want to go back to IgG.
Other than that, Bob has made a couple of excellent points, really worth stressing: 1) Size matters. There is a paper, if I am remembering right, by Rick Giberson from Ted Pella, where they show how going from one size to the next, the labeling density decreases almost in half. 5-6 nm is often too small for screening extended areas, otherwise use the smallest size you can see. (Ultrasmall is very convenient, because you can benefit from high labeling density and then grow the label as big as you need. Silver enhancement is actually very simple these days, but still, for a first try with a rabbit primary, I would definitely go with 5-10 nm PAG. Too bad there is no ultrasmall PAG.) 2) Do not overblock. You want to establish some binding first, then deal with the background, if you have it. Good controls really help :) An ideal control is cells that lack the protein in question but are otherwise isogenic - any gold you see over those is either background or cross-reactivity. Using an unrelated rabbit polyclonal instead of your primary would be your second best.
Brands - in the US, you can buy EM gold conjugates from a number of suppliers, but they all will be made by either Aurion or BBI International (I'll be glad to hear I'm wrong). There is also Jackson Immunoresearch, excellent conjugates, but I am not sure if they offer Protein A... All three are very reputable brands. I have had good experience with Aurion's conjugates, while many of my colleagues use BBI. You may or may not have the same choices in Australia, but make sure to insist on chilled overnight shipping, especially with Protein A.
Good luck, Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
Begin forwarded message:
} From: underwoo-at-u.washington.edu } Date: October 10, 2007 11:56:52 AM EDT } To: vladislav_speransky-at-nih.gov } Subject: [Microscopy] Re: Immunogold reagents (protein A-gold, IgG- } gold) } Reply-To: underwoo-at-u.washington.edu } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Scott, } } For species specific gold conjugated secondaries against rabbit } primaries we have used both BBI } international available through Ted Pella and Aurion avaliable } through EMS (Electron Microscopy } Sciences) with excellent results in sizes from ultrasmall (Aurion, } requiring enhancement) through 40nm. } Labelling frequency tends to go down as the size of gold increases. } We have little experience with } protein A. Two things that I have noticed that have been helpful. } 1) Use PBS rather than TBS for a buffer } as the TBS incompatibility with the antibody buffer will tend to } cause clustering of the antibodies not } noticable at the light level. 2) We use only BSA not species } specific serum like goat serum, for blocking. } The goat serum tends to be too good and the signal is reduced by a } factor of ten for most of our } antibodies. } } Robert Underwood } University of Washington } Dermatology Research Center } Seattle, WA } } } ---------- Forwarded message ---------- } Date: Wed, 10 Oct 2007 00:44:38 -0500 } X-from: scott.coutts-at-med.monash.edu.au } To: underwoo-at-u.washington.edu } Subject: [Microscopy] viaWWW: Immunogold reagents (protein A-gold, } IgG-gold) } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/ } MicroscopyListserver/MLFormMail.html } ---------------------------------------------------------------------- } ----- } Remember this posting is most likely not from a Subscriber, so when } replying } please copy both scott.coutts-at-med.monash.edu.au as well as the } MIcroscopy Listserver } ---------------------------------------------------------------------- } ----- } } Email: scott.coutts-at-med.monash.edu.au } Name: Scott Coutts } } Organization: Monash University } } Title-Subject: [Filtered] Immunogold reagents (protein A-gold, IgG- } gold) } } Question: Hi Everyone, } } Can anyone make a recommendation for a gold label for } immunolabelling? We don't want to conjugate } our own gold. We'll be labelling whole cells and sections in LR } white. The primary is polyclonal rabbit. } Other than the species specificity issues, is there a general } consensus on whether IgG or protein A is } better for labelling? What about brands? } } Cheers, } } Scott. } } Login Host: 130.194.13.102 } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== } 9, 11 -- From zaluzec-at-microscopy.com Wed Oct 10 00:40:27 2007 } 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com } [206.69.208.22]) } 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id l9A5eQxY005811 } 9, 11 -- for {microscopy-at-microscopy.com} ; Wed, 10 Oct 2007 } 00:40:26 -0500 } 9, 11 -- Mime-Version: 1.0 } 9, 11 -- Message-Id: {p06240801c332162f007f-at-[206.69.208.22]} } 9, 11 -- Date: Wed, 10 Oct 2007 00:40:24 -0500 } 9, 11 -- To: microscopy-at-microscopy.com } 9, 11 -- From: scott.coutts-at-med.monash.edu.au (by way of } MicroscopyListserver) } 9, 11 -- Subject: viaWWW: Immunogold reagents (protein A-gold, IgG- } gold) } 9, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers============================== } } } } } ==============================Original } Headers============================== } 22, 22 -- From underwoo-at-u.washington.edu Wed Oct 10 10:55:19 2007 } 22, 22 -- Received: from mxout7.cac.washington.edu } (mxout7.cac.washington.edu [140.142.32.178]) } 22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id l9AFtJLX026475 } 22, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 10 Oct 2007 } 10:55:19 -0500 } 22, 22 -- Received: from hymn14.u.washington.edu } (hymn14.u.washington.edu [140.142.4.104]) } 22, 22 -- by mxout7.cac.washington.edu (8.13.7+UW06.06/8.13.7 } +UW07.09) with ESMTP id l9AFtI8g012609 } 22, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 } verify=NO) } 22, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 10 Oct 2007 } 08:55:18 -0700 } 22, 22 -- Received: from localhost (localhost [127.0.0.1]) } 22, 22 -- by hymn14.u.washington.edu (8.13.7+UW06.06/8.13.7 } +UW07.09) with ESMTP id l9AFtIj4002591 } 22, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 10 Oct 2007 } 08:55:18 -0700 } 22, 22 -- X-Auth-Received: from [128.208.106.80] by } hymn14.u.washington.edu via HTTP; Wed, 10 Oct 2007 08:55:18 PDT } 22, 22 -- Date: Wed, 10 Oct 2007 08:55:18 -0700 (PDT) } 22, 22 -- From: Robert A Underwood {underwoo-at-u.washington.edu} } 22, 22 -- To: Microscopy List {Microscopy-at-MSA.Microscopy.Com} } 22, 22 -- Subject: [Microscopy] Re: Immunogold reagents (protein A- } gold, IgG-gold) } 22, 22 -- (fwd) } 22, 22 -- Message-ID: {Pine.LNX. } 4.43.0710100855180.30666-at-hymn14.u.washington.edu} } 22, 22 -- MIME-Version: 1.0 } 22, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed } 22, 22 -- X-PMX-Version: 5.3.3.310218, Antispam-Engine: } 2.5.2.313940, Antispam-Data: 2007.10.10.82905 } 22, 22 -- X-Uwash-Spam: Gauge=IIIIIII, Probability=7%, } Report='__CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __HAS_MSGID } 0, __MEDS_PLAIN_MEDICATION 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, } __SANE_MSGID 0, __STOCK_PHRASE_24 0' } ==============================End of - } Headers==============================
==============================Original Headers============================== 7, 23 -- From vladislav_speransky-at-nih.gov Fri Oct 12 16:58:49 2007 7, 23 -- Received: from nihrelayxway.hub.nih.gov (nihrelayxway.hub.nih.gov [128.231.90.106]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9CLwmQ6015480 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 12 Oct 2007 16:58:48 -0500 7, 23 -- X-IronPortListener: NIH_Relay 7, 23 -- X-SBRS: None 7, 23 -- X-IronPort-AV: E=Sophos;i="4.21,268,1188792000"; 7, 23 -- d="scan'208";a="556781498" 7, 23 -- Received: from helix.nih.gov ([128.231.2.3]) 7, 23 -- by nihrelayxway.hub.nih.gov with ESMTP; 12 Oct 2007 17:58:48 -0400 7, 23 -- Received: from [156.40.102.124] ([156.40.102.124]) 7, 23 -- by helix.nih.gov (8.13.6/8.13.6/2SCANNER) with ESMTP id l9CLwmFi40354790 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 12 Oct 2007 17:58:48 -0400 (EDT) 7, 23 -- Mime-Version: 1.0 (Apple Message framework v752.2) 7, 23 -- References: {200710101556.l9AFuq6E028044-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 7, 23 -- Message-Id: {79CECBFA-509D-413B-BEAA-50D0001436B7-at-nih.gov} 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 7, 23 -- Subject: Fwd: [Microscopy] Re: Immunogold reagents (protein A-gold, IgG-gold) 7, 23 -- Date: Fri, 12 Oct 2007 17:58:25 -0400 7, 23 -- To: Microscopy-at-microscopy.com 7, 23 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
RESEARCH RESOURCES CENTER, UNIVERSITY OF ILLINOIS AT CHICAGO
Microscopy Specialist/Research Specialist
The Electron Microscopy Service of the Research Resources Center (RRC) at the University of Illinois at Chicago has an open position for a Microscopy Specialist. The facility provides electron and laser microscopy and surface analysis services for the university research community and external organisations from two sites on the campus. The open position is in the RRC-East facility, which specializes in physical and materials science fields. The east side facility includes a JEOL JEM-3010 and JEOL JEM-100CX TEMs, JEOL JEM-2010F and VG HB601 STEMs, Kratos AXIS-165 XPS and a Renishaw Raman Spectrometer,.
The person we are looking for should have a bachelor's degree minimum and preferably a master's degree or higher in physical sciences, engineering or a related field, with more than one year's experience in laser and electron microscopy. They will supervise the operation of the specimen preparation area, including record keeping and maintenance and will assist/supervise in the day to day running of the Electron Microscopes, Surface Analysis and Raman. Interpersonal/ Communications skills are important, as this individual will work with users, provide technical advice and demonstrate how microscopy can advance their research.
Review of applications will commence immediately. For consideration, interested parties should send an application letter, complete curriculum vitae, and the names and addresses of three references to:
Alan W Nicholls, Ph.D. (nicholls-at-uic.edu) Research Resources Center – East (m/c 337) University of Illinois at Chicago 845 West Taylor Street. Chicago, Illinois 60607-7058
Voice: (312) 996-1227 Fax: (312) 996-0539
UIC is an EEO/AA.
Alan W Nicholls, PhD Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 110 Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
------------------------------------------------------------------- MEETING ANNOUNCEMENT: Workshop on Advanced Electron Microscopy in Materials Physics Date : November 7-8th, 2007 Location: CFN, Brookhaven National Laboratory (NY) LINK: https://www.bnl.gov/aemworkshop/
DESCRIPTION: The two-day workshop is structured to stimulate scientific exchanges and explore new capabilities. Join your colleagues at BNL's world class facilities for an exciting two days of invited talks, informal discussions and technical exchanges. While the scientific themes of the workshop are focused on aberration corrected STEM and EELS, other advanced electron microscopy methods and materials physics applications are also incorporated. Invited speakers include P. Batson, R. Borkowski , N. Browning, M. Haider, A. Kirkland, B. Kabius, D. Muller, S. Pennycook, H. Rose, M. Ruehle and R. Tromp.
There will be an opportunity check out the newly installed 200kV cold field emission, probe-corrected, dedicated-STEM equipped with an ultra-fast high resolution EELS, and tour the new Center for Functional Nanomaterials!
TO ATTEND, please REGISTER ASAP: https://www.bnl.gov/aemworkshop/ (70 attendee limit, registration will be first-come first serve) Note: 2-3 weeks advanced notice required for non-US citizens (required to process visitor/guest registration)
Workshop Organizers: Konrad Jarausch (Hitachi High Technologies America) Yimei Zhu (Brookhaven National Laboratories)
Does anyone know the type of insulated wires typically used inside SEM microscope chambers. Specifically, I am interested in knowing the type of material used in the insulation so that it doesn't outgas. Brand name and source would be a great plus.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
==============================Original Headers============================== 3, 20 -- From walck-at-southbaytech.com Mon Oct 15 13:29:25 2007 3, 20 -- Received: from flpi185.prodigy.net (flpi185.sbcis.sbc.com [207.115.20.187]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9FITO56000321 3, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Oct 2007 13:29:25 -0500 3, 20 -- X-ORBL: [64.169.217.123] 3, 20 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 3, 20 -- by flpi185.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id l9FITfhZ016166 3, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Oct 2007 11:29:42 -0700 3, 20 -- From: "Scott Walck" {walck-at-southbaytech.com} 3, 20 -- To: {Microscopy-at-microscopy.com} 3, 20 -- Subject: Vacuum compatible Insulated wires -type and source 3, 20 -- Date: Mon, 15 Oct 2007 11:27:40 -0700 3, 20 -- Message-ID: {006601c80f59$12518ee0$7801a8c0-at-dynamicbl8uno3} 3, 20 -- MIME-Version: 1.0 3, 20 -- Content-Type: text/plain; 3, 20 -- charset="US-ASCII" 3, 20 -- Content-Transfer-Encoding: 7bit 3, 20 -- X-Mailer: Microsoft Office Outlook 11 3, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 3, 20 -- Thread-Index: AcgPWRG0o1mL3Ql7TzqcjLvtc9ll2Q== ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both amgusman-at-ucdavis.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: amgusman-at-ucdavis.edu Name: Andrea Gusman
Title-Subject: [Filtered] Philips CM-12 TEM
Question: We currently have a Philips CM-12 TEM and the CRT screen on it is no longer working. We are looking at a replacement cost of $6,000. Does anyone have any experience working with these type of monitors? Replacing them or hooking up a video monitor? Or does anyone have any suggestions? Thank you.
I don't know specifically what manufacturers use in SEM vacuum chambers; however, Radio Shack sell several sizes (26, 28, and 30 gauge, I think) of "wrapping wire' that I have used with good results in vacuum applications. This is single-strand copper wire that is coated with a tough, colored varnish (probably a Formvar varnish) for insulation. I don't know the exact limits, but I believe the insulation is good for up to 110 volts. It isn't very expensive, so you might try a roll to see if it meets your needs electrically. There should be no problem whatsoever insofar as vacuum compatibility is concerned. Wil -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 1, 14 -- From bigelow-at-umich.edu Mon Oct 15 14:47:10 2007 1, 14 -- Received: from skycaptain.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.160]) 1, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9FJlAOx026329 1, 14 -- for {microscopy-at-microscopy.com} ; Mon, 15 Oct 2007 14:47:10 -0500 1, 14 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 1, 14 -- BY skycaptain.mr.itd.umich.edu ID 4713C3BD.55663.1884 ; 1, 14 -- 15 Oct 2007 15:47:09 -0400 1, 14 -- Mime-Version: 1.0 1, 14 -- Message-Id: {p06210201c3397275e04f-at-[141.212.131.221]} 1, 14 -- Date: Mon, 15 Oct 2007 15:47:07 -0400 1, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 1, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 14 -- Subject: [Microscopy] RE: wires in vacuum 1, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
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Email: pmoeck-at-pdx.edu Name: Peter Moeck
Organization: Portland State University
Title-Subject: [Filtered] search for Postdoc Z-STEM/EELS up to 3 years West coast based
Question: Postdoc, USA West-coast based, within a collaboration between Portland State University, the University of California at Davis, the Lawrence Livermore National Laboratory, the University of Washington at Seattle, and the Pacific North West National Laboratory. Initially for one year at $35,500 (plus the usual approximately 50 % fringe benefits: health insurance, retirement benefits, etc.), available immediately, extendable up to 3 years by mutual agreement since funding is already secured ! A group of collaborators that are based at Portland State University, the University of California at Davis, and the University of Washington at Seattle is seeking a (male/female) postdoc for a project on Crystallographic and spectroscopic analyses of ferromagnetic semiconducting nanoparticle aggregates with Curie temperatures well above room temperature. A background in materials physics, materials chemistry, crystallography, or materials science and engineering is required. Familiarity with Z-contrast (HAADF) imaging in scanning transmission electron microscopes and associated electron energy loss spectroscopy are essential. Skills in high resolution phase-contrast transmission electron microscopy, electron diffraction and crystallography, and crystallographic image processing will be appreciated. Most of the work will actually be done in Prof. Nigel D. Browningís group at the University of California at Davis. Occasional travel to the other involved laboratories is expected. The search will be open until the position has been filled. Applications (CV, list of referees, list of publications, etc.) should be sent to either or both of the following collaborators: Prof. Peter Moeck Department of Physics Portland State University P.O. Box 751 Portland, Oregon 97207-0751 Tel.: 503 725 4227, Fax: 503 725 2815, e-mail: pmoeck-at-pdx.edu research group: Nanocrystallography Group and open-access crystallographic databases
and/or
Prof. Nigel D. Browning Department of Chemical Engineering and Materials Science University of California-Davis One Shields Ave Davis, CA 95616 Tel: 530-754-5358, Fax: 530-752-9554, e-mail: nbrowning-at-ucdavis.edu research group: Interface Physics Group
------------------------------------------------------------------------------- also to be checked out is this information on the overall project leader: Prof. Daniel R. Gamelin, his research group: Gamelin Research Group, and his groupís selected publications in the respective field
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Email: susan.trant-at-viha.ca Name: Susan Trant
Organization: Vancouver Island Health Authority
Title-Subject: [Filtered] Film for EM Microscope
Question: I have an older JOEL TEM that uses 35 mm film that comes in 500 m roll form. Does anyone out there know where I can purchase more? I have been experimenting with Ilford 35 mm black and white film, but have not been successful in coming up with the right exposure and film development times.
X-from plastic materials, commonly used as wire isolators, best vacuum properties belong to Polyimide (or Kapton) - it can go to UHV and would withstand long 200C bakeouts without any degradation. The drawback is that polyimide wire is not very flexible and will not allow sharp bending; it is also quite pricy.
Polyimide-isolated wire is available from Accuglass, wire and coax, other vendors possible.
http://accuglassproducts[dot]com/home.php?cat=251
Second common isolator with excellent vacuum properties is Teflon (PTEE), available form above, and many other vendors. Teflon wire would not take you to 10E-10 Torr, but it works very well down to 10E-8, way more flexible then Kapton and less expensive:
Feel free to contact me offline if you need advice for some specific application, or if you need to go to UHV below 10E-10 Torr.
I have no connection to the Accuglass or Planetengineers, other then being a satisfied customer.
Cheers, Valery ====================== Particle Beam Systems & Technology
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Does anyone know the type of insulated wires typically used inside SEM microscope chambers. Specifically, I am interested in knowing the type of material used in the insulation so that it doesn't outgas. Brand name and source would be a great plus.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
==============================Original Headers============================== 3, 20 -- From walck-at-southbaytech.com Mon Oct 15 13:29:25 2007 3, 20 -- Received: from flpi185.prodigy.net (flpi185.sbcis.sbc.com [207.115.20.187]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9FITO56000321 3, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Oct 2007 13:29:25 -0500 3, 20 -- X-ORBL: [64.169.217.123] 3, 20 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) 3, 20 -- by flpi185.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id l9FITfhZ016166 3, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Oct 2007 11:29:42 -0700 3, 20 -- From: "Scott Walck" {walck-at-southbaytech.com} 3, 20 -- To: {Microscopy-at-microscopy.com} 3, 20 -- Subject: Vacuum compatible Insulated wires -type and source 3, 20 -- Date: Mon, 15 Oct 2007 11:27:40 -0700 3, 20 -- Message-ID: {006601c80f59$12518ee0$7801a8c0-at-dynamicbl8uno3} 3, 20 -- MIME-Version: 1.0 3, 20 -- Content-Type: text/plain; 3, 20 -- charset="US-ASCII" 3, 20 -- Content-Transfer-Encoding: 7bit 3, 20 -- X-Mailer: Microsoft Office Outlook 11 3, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 3, 20 -- Thread-Index: AcgPWRG0o1mL3Ql7TzqcjLvtc9ll2Q== ==============================End of - Headers==============================
==============================Original Headers============================== 19, 24 -- From vray-at-partbeamsystech.com Mon Oct 15 21:35:02 2007 19, 24 -- Received: from smtp113.plus.mail.re1.yahoo.com (smtp113.plus.mail.re1.yahoo.com [69.147.102.76]) 19, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l9G2Z2sk009107 19, 24 -- for {microscopy-at-microscopy.com} ; Mon, 15 Oct 2007 21:35:02 -0500 19, 24 -- Message-Id: {200710160235.l9G2Z2sk009107-at-ns.microscopy.com} 19, 24 -- Received: (qmail 74277 invoked from network); 16 Oct 2007 02:35:01 -0000 19, 24 -- Received: from unknown (HELO cp1198275a) (partbeamsystech-at-75.68.110.151 with login) 19, 24 -- by smtp113.plus.mail.re1.yahoo.com with SMTP; 16 Oct 2007 02:35:01 -0000 19, 24 -- X-YMail-OSG: 2Y7uJWMVM1mGEffoP3B8Qkok1GFYQBHm51eaki9ftCod3mqR9qio4AvD9M.L._60YVhZqDUC9f8sTV3n3b_7QlzrmOlWFD6hrFgxgfZ6Sxv1Sp4QOrvq2LiNmpKoWBj5O1xKTMNaQnLtxeNGx4IWpcFNeXVDOGVjUvux.vALLSM- 19, 24 -- Reply-To: {vray-at-partbeamsystech.com} 19, 24 -- From: "Valery Ray" {vray-at-partbeamsystech.com} 19, 24 -- To: {walck-at-southbaytech.com} 19, 24 -- Cc: {microscopy-at-microscopy.com} 19, 24 -- Subject: RE: [Microscopy] Vacuum compatible Insulated wires -type and source 19, 24 -- Date: Mon, 15 Oct 2007 22:37:08 -0400 19, 24 -- Organization: PBST / MEO Engineering 19, 24 -- MIME-Version: 1.0 19, 24 -- Content-Type: text/plain; 19, 24 -- charset="us-ascii" 19, 24 -- Content-Transfer-Encoding: 7bit 19, 24 -- X-Mailer: Microsoft Office Outlook, Build 11.0.5510 19, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 19, 24 -- Thread-Index: AcgPWXDbL2TS1pZnTJmxSzoKZG1dOwAP9QYQ 19, 24 -- In-Reply-To: {200710151830.l9FIUKVY002157-at-ns.microscopy.com} ==============================End of - Headers==============================
2) non-insulated wire with ceramic beads, for extreme UHV and high T bake-out.
One good source for (2) is www.omega.com , as well as usual vac. suppliers ( www.lesker.com , etc.) Teflon wire is widely available, in great variety.
What specific vacuum, temperatures, current, voltage are you looking for? Is flexibility required?
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com ----- Original Message ----- X-from: {walck-at-southbaytech.com} To: {vitalylazar-at-att.net} Sent: Monday, October 15, 2007 2:30 PM
In UHV applications, we have mostly used nude wires, insulated with alumina tubes (single or multi bored) or beads. It's the best way to know what one do. No organics and only stable materials.
For HV application and some UHV too, as other have mentionned, the commonly used are silicon, teflon and kapton.
The kapton/polyimide kind is a better price/performences compromise, with the main drawback that it catches water vapor and outgases then a lot during the pumping down. We have some such home-made in-vacuum Helmoltz coils, which need to be "baked" with a strong working current, otherwise they slow down the pumping. It should not be an problem in HV, as long as there isn't some length.
We tried once a ceramic coated wire, made by a German company (www.detakta.de), which was fine but expensive and sitff. The polyimide one came from the same manufacturer.
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
walck-at-southbaytech.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Does anyone know the type of insulated wires typically used inside SEM } microscope chambers. Specifically, I am interested in knowing the type of } material used in the insulation so that it doesn't outgas. Brand name and } source would be a great plus. } } -Scott } } Scott D. Walck, Ph.D. } Technical Director } South Bay Technology, Inc. } 1120 Via Callejon } San Clemente, CA 92673 } } US Toll Free: 1-800-728-2233 } Tel: (949) 492-2600 } Fax: (949) 492-1499 } } www.southbaytech.com } walck-at-southbaytech.com } } } ==============================Original Headers============================== } 3, 20 -- From walck-at-southbaytech.com Mon Oct 15 13:29:25 2007 } 3, 20 -- Received: from flpi185.prodigy.net (flpi185.sbcis.sbc.com [207.115.20.187]) } 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9FITO56000321 } 3, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Oct 2007 13:29:25 -0500 } 3, 20 -- X-ORBL: [64.169.217.123] } 3, 20 -- Received: from dynamicbl8uno3 (adsl-64-169-217-123.dsl.lsan03.pacbell.net [64.169.217.123]) } 3, 20 -- by flpi185.prodigy.net (8.13.8 out.dk.spool/8.13.8) with ESMTP id l9FITfhZ016166 } 3, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 15 Oct 2007 11:29:42 -0700 } 3, 20 -- From: "Scott Walck" {walck-at-southbaytech.com} } 3, 20 -- To: {Microscopy-at-microscopy.com} } 3, 20 -- Subject: Vacuum compatible Insulated wires -type and source } 3, 20 -- Date: Mon, 15 Oct 2007 11:27:40 -0700 } 3, 20 -- Message-ID: {006601c80f59$12518ee0$7801a8c0-at-dynamicbl8uno3} } 3, 20 -- MIME-Version: 1.0 } 3, 20 -- Content-Type: text/plain; } 3, 20 -- charset="US-ASCII" } 3, 20 -- Content-Transfer-Encoding: 7bit } 3, 20 -- X-Mailer: Microsoft Office Outlook 11 } 3, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } 3, 20 -- Thread-Index: AcgPWRG0o1mL3Ql7TzqcjLvtc9ll2Q== } ==============================End of - Headers============================== }
==============================Original Headers============================== 10, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Oct 16 02:18:48 2007 10, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.156]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9G7IlJe006889 10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 16 Oct 2007 02:18:48 -0500 10, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 10, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id l9G7IiFn014838 10, 29 -- for {microscopy-at-microscopy.com} ; Tue, 16 Oct 2007 09:18:44 +0200 (CEST) 10, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 10, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id D2076424008 10, 29 -- for {Microscopy-at-Microscopy.Com} ; Tue, 16 Oct 2007 09:17:54 +0200 (CEST) 10, 29 -- Message-ID: {471465A9.60006-at-ipcms.u-strasbg.fr} 10, 29 -- Date: Tue, 16 Oct 2007 09:18:01 +0200 10, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 10, 29 -- User-Agent: Thunderbird 1.5.0.13 (X11/20070824) 10, 29 -- MIME-Version: 1.0 10, 29 -- To: Microscopy-at-microscopy.com 10, 29 -- Subject: Re: [Microscopy] Vacuum compatible Insulated wires -type and source 10, 29 -- References: {200710151834.l9FIYvVT009061-at-ns.microscopy.com} 10, 29 -- In-Reply-To: {200710151834.l9FIYvVT009061-at-ns.microscopy.com} 10, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- X-IPCMS-MailScanner: Found to be clean 10, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 10, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.156]); Tue, 16 Oct 2007 09:18:44 +0200 (CEST) 10, 29 -- X-Virus-Scanned: ClamAV 0.88.7/4540/Sun Oct 14 03:43:55 2007 on mr6.u-strasbg.fr 10, 29 -- X-Virus-Status: Clean 10, 29 -- X-Spam-Status: No, score=-0.1 required=5.0 tests=AWL autolearn=disabled 10, 29 -- version=3.1.8 10, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr6.u-strasbg.fr ==============================End of - Headers==============================
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Email: k.levick-at-unsw.edu.au Name: Katie Levick
Organization: Electron Microscope Unit, University of New South Wales
Title-Subject: [Filtered] TEM - film plates for Philips cm - series microscopes
Question: Hi everyone,
I am wondering if anyone has any film carrier plates that fit the Philips cm series microscopes which they would be willing to sell/give away? We use 3.25x4inch plate film.
Imaging Scientist - St. Jude Children's Research Hospital.
Currently, St. Jude Children's Research Hospital has an opening for an Imaging Scientist (Job Number 14556) in the Immunology Department. The successful candidate would be responsible for managing an imaging facility focused on interdisciplinary, multi-user microscopy. Experience with advance microscopy techniques and methodologies, including live cell imaging, FRET, FLIM, FRAP, FCS and TIRF is highly desired.
Requirements: A Bachelor's degree in an appropriate scientific field plus a minimum of ten (10) years (post-degree) of relevant and productive work experience is required or, A Master's degree in an appropriate scientific field plus a minimum of nine (9) years (post-degree) of relevant and productive work experience is required or, A PhD in an appropriate scientific field plus five (5) years (post-degree) of relevant and productive work experience is required.
To apply please visit our Web site www.stjude.org/jobs
St. Jude Children's Research Hospital is an Equal Opportunity Employer.
-- Samuel A. Connell Director of Light Microscopy Cell & Tissue Imaging Center St. Jude Children's Research Hospital 332 North Lauderdale St., E7061 Memphis, TN 38105-2794 (901) 495-2536 samuel.connell-at-stjude.org
==============================Original Headers============================== 11, 23 -- From Samuel.Connell-at-STJUDE.ORG Tue Oct 16 10:20:45 2007 11, 23 -- Received: from mgate2.stjude.org (mgate2.stjude.org [192.55.208.21]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9GFKjP9019529 11, 23 -- for {Microscopy-at-Microscopy.com} ; Tue, 16 Oct 2007 10:20:45 -0500 11, 23 -- X-SEF-Processed: 5_0_0_910__2007_10_16_10_20_45 11, 23 -- X-SEF-SJMEMMS4: 1 11, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 23 -- Content-class: urn:content-classes:message 11, 23 -- MIME-Version: 1.0 11, 23 -- Content-Type: text/plain; 11, 23 -- charset="us-ascii" 11, 23 -- Subject: Imaging Scientist Position Available 11, 23 -- Date: Tue, 16 Oct 2007 10:20:44 -0500 11, 23 -- Message-ID: {67C5DCA3F0C94E4186175E682FBB36231813A49D-at-SJMEMXMB01.stjude.sjcrh.local} 11, 23 -- X-MS-Has-Attach: 11, 23 -- X-MS-TNEF-Correlator: 11, 23 -- Thread-Topic: Imaging Scientist Position Available 11, 23 -- Thread-Index: AcgQCB8+tKIDqrMRTzagO5P8vR5a5Q== 11, 23 -- From: "Connell, Samuel" {Samuel.Connell-at-STJUDE.ORG} 11, 23 -- To: {Microscopy-at-Microscopy.com} 11, 23 -- X-OriginalArrivalTime: 16 Oct 2007 15:20:45.0227 (UTC) FILETIME=[1F60A7B0:01C81008] 11, 23 -- Content-Transfer-Encoding: 8bit 11, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l9GFKjP9019529 ==============================End of - Headers==============================
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Email: Darkmatterfound-at-gmail.com Name: Victor Lo
Organization: ANSTO
Title-Subject: [Filtered] Staining samples
Question: Hi there!
Just wondering can provide me with any suggestion for staining starch for TEM sample? I have no back-ground for preparing biological samples ... so any suggestions, tricks and tips would be fantastic!!
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Email: joef-at-the-mandp-lab.com Name: Joe Foroughi
Organization: The M&P Lab
Title-Subject: [Filtered] Glass Fibers
Question: Is there an easy derivitization protocal to use for glass fibers to make them repel each other, so that they will be nicely distributed and not overlapping for image analysis? A paper from 1979 tells of an aqueous mixture of 0.25% methyl trimethoxysilane, 0.25% of a cationic lubricant called Softener-X (Cat-X) from Refined Onyx Corporation, and acetic acid to pH=4. I can't find any information on the cationic lubricant.
I have to move my EM Facility somewhere else on campus because we have out grown our existing location and environmental conditions (Vibration, EMI, and Acoustic) are no longer compatible with EM´s. The chosen site will be renovated for an EM Facility but the question comes as to "Where"?
We have had site survey´s done on two potential locations. One location was horrible (Problems out of spec for vibration, EMI-AC, EMI-DC, and Acoustic). The second was poor (out of spec for EMI-AC for 200 Kv TEM and high-end SEM, with distance issues for EMI-DC). Since we are not going to build a new building just for EM (unreasonable of my administration I know), where do I look for physically compatible space? I would like to solicit comments, discussion and suggestions for developing a search criteria for EM Facility locations.
I am hoping that this criteria maybe of use for more than myself and my facility, as I believe more and more of us will be facing similar issues. "Normal" EM´s are moving into higher and higher capabilities, i.e. 200 - 300 KV TEM´s and ultra low voltage FEG SEM´s are becoming the new "standard", and the microscope environmental specs are getting more and more stringent. Manufacturers do not seem readily jumping to handle/correct/compensate in their engineering designs to deal with these issues and the fact that the "standard" microscope still needs to be installed within an existing research building environment. Note: I am not addressing the ultra-highend bleeding edge scopes (i.e. CS-corrected, monochomatic, FEG, count-the- neutrons-in- the-atoms scopes) - yes, there will always be an in house "support" price to pay for the bleeding edge. I am talking about the fact that the "standard" microscope still needs to be installed within an existing research building environment, and this fact its seems is being ignored my the manufacturers. (Albeit: a recent mailing from one manufacturer does somewhat acknowledge the issues and states their new design does allow for "Reduced installation requirements for acoustic and temperature variations" - the easier environmental issues to correct for and is part of a multi- million dollar scope). Third party add-on anti-vibration or EMI cancellation systems are not going to be as good as something inherently designed into the scope and does allow for the "Its not our microscope that´s the problem its their EMI cancellation system" kind of endless loops. Further more, due to cross-interference of EMI cancellation systems, if you have more than one scope, the scopes need to be space prohibitively far apart (20-25m spacing? Four? Five EM´s?).
Criteria for consideration:
Obviously, there can be no hard and fast rules, and every site needs to ultimately be tested, but the list of potential sites can be reduced or prioritized.
If the options exist: Sandy soil is highly preferred. Clay soils are not good. Are there other soil type considerations? (Wet or flood prone areas are definitely not desired, eh?)
For acoustics and vibration: Older, underground (basements), stone, cinder block, concrete building constructions are good. Interior walls of same or wood studded drywall or plaster are good. New, steel studded dry walls are not generally good - they offer little to no support, often serve as vibration/acoustic resonators. (Is there a EMI issue with Steel studs?)
Catch-22 Older maybe bad due to electro-magnetic interference (EMI) issues, particularly old (and new) science buildings as they tend to add additional electrical feeds every which way which causes EMI. Science buildings seem to be bad because there are (albeit physical sciences vs. math or cultural anthropology). Higher ceilings are better (4-5 meters), as electrical and water supply lines which run in the interstitial floor space can cause EMI problems but these drop off quickly with, and the larger room air volume buffers air pressure and temperature.
Wiring: We have to have it, but other than staying away from major building power distribution / supplies waht needs to be considered? And how far from major distributions is reasonable to look at? Question: Twisted power feed cables are ideal, but are they critical?
Wi-fi has been discussed on the microscopy listserv and seems not to be an issue provided you do not mounted the antenna´s on the column.
New Buildings: Built with General construction can be horrible for EMI (lots of electrical supplies for all of 20th century equipment), acoustic (ventilation and steel stud walls), vibrations (lighter cheaper construction methods). BUT if planned for can be controlled and eliminated (?) - however, you (the EM people) have to constantly confirm that construction is being done to spec.
Elevators are not good near EM's, and near = 5 to 8m upto10 to15 meters minimum maybe? I am trying to find out. HOWEVER, there are big problems with a basement level EM Facility which has no elevator access. How do you get the scopes in? Or liquid nitrogen and compressed gas cylinders? Are hydraulic or cable elevators better? (One shaft vs counter weight).
Roads: Lighter the traffic in terms of numbers and vehicle mass is better. Railroads
What have I gotten wrong? What have I ignored? What key factors should be considered?
Campus Politics: Get and keep the biggest research guns and biggest departments happy users, and make the Admin knows you do a good job and keep as many as possible happy.
And so obviously the best location would be a concrete and brick bunker underneath the Arizona desert with no other buildings within 1 kilometer served by a dirt mule path is the only way to go right? Anybody have an old missile silo? Oh but then there´s all those massive steel doors and springs . . .
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 21, 23 -- From edelmare-at-muohio.edu Wed Oct 17 11:01:54 2007 21, 23 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 21, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9HG1ral021760 21, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 17 Oct 2007 11:01:54 -0500 21, 23 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 21, 23 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l9HG1lIF032120 21, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 17 Oct 2007 12:01:47 -0400 21, 23 -- Received: from [192.168.1.23] ([134.53.14.105]) 21, 23 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l9HG1lWK017219 21, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 17 Oct 2007 12:01:47 -0400 21, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 21, 23 -- To: microscopy-at-Microscopy.com 21, 23 -- Date: Wed, 17 Oct 2007 12:01:47 -0400 21, 23 -- MIME-Version: 1.0 21, 23 -- Subject: Where to relocate an EM Facility 21, 23 -- Message-ID: {4715F9AB.19955.18B62727-at-edelmare.muohio.edu} 21, 23 -- Priority: normal 21, 23 -- X-mailer: Pegasus Mail for Windows (4.41) 21, 23 -- Content-type: text/plain; charset=ISO-8859-1 21, 23 -- Content-description: Mail message body 21, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 21, 23 -- Content-Transfer-Encoding: 8bit 21, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id l9HG1ral021760 ==============================End of - Headers==============================
At a recent meeting a manufacturer's rep said it took £500,000 (about $1,000,000) to get a room ready for their new top of the range TEM. I suggest we buy lottery tickets and get something like the FEI Titan which comes in a controlled box. Then you can put it anywhere, perhaps.
Dave
-----Original Message----- X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu] Sent: 17 October 2007 17:12 To: David Patton
I have to move my EM Facility somewhere else on campus because we have out grown our existing location and environmental conditions (Vibration, EMI, and Acoustic) are no longer compatible with EM´s. The chosen site will be renovated for an EM Facility but the question comes as to "Where"?
We have had site survey´s done on two potential locations. One location was horrible (Problems out of spec for vibration, EMI-AC, EMI-DC, and Acoustic). The second was poor (out of spec for EMI-AC for 200 Kv TEM and high-end SEM, with distance issues for EMI-DC). Since we are not going to build a new building just for EM (unreasonable of my administration I know), where do I look for physically compatible space? I would like to solicit comments, discussion and suggestions for developing a search criteria for EM Facility locations.
I am hoping that this criteria maybe of use for more than myself and my facility, as I believe more and more of us will be facing similar issues. "Normal" EM´s are moving into higher and higher capabilities, i.e. 200 - 300 KV TEM´s and ultra low voltage FEG SEM´s are becoming the new "standard", and the microscope environmental specs are getting more and more stringent. Manufacturers do not seem readily jumping to handle/correct/compensate in their engineering designs to deal with these issues and the fact that the "standard" microscope still needs to be installed within an existing research building environment. Note: I am not addressing the ultra-highend bleeding edge scopes (i.e. CS-corrected, monochomatic, FEG, count-the- neutrons-in- the-atoms scopes) - yes, there will always be an in house "support" price to pay for the bleeding edge. I am talking about the fact that the "standard" microscope still needs to be installed within an existing research building environment, and this fact its seems is being ignored my the manufacturers. (Albeit: a recent mailing from one manufacturer does somewhat acknowledge the issues and states their new design does allow for "Reduced installation requirements for acoustic and temperature variations" - the easier environmental issues to correct for and is part of a multi- million dollar scope). Third party add-on anti-vibration or EMI cancellation systems are not going to be as good as something inherently designed into the scope and does allow for the "Its not our microscope that´s the problem its their EMI cancellation system" kind of endless loops. Further more, due to cross-interference of EMI cancellation systems, if you have more than one scope, the scopes need to be space prohibitively far apart (20-25m spacing? Four? Five EM´s?).
Criteria for consideration:
Obviously, there can be no hard and fast rules, and every site needs to ultimately be tested, but the list of potential sites can be reduced or prioritized.
If the options exist: Sandy soil is highly preferred. Clay soils are not good. Are there other soil type considerations? (Wet or flood prone areas are definitely not desired, eh?)
For acoustics and vibration: Older, underground (basements), stone, cinder block, concrete building constructions are good. Interior walls of same or wood studded drywall or plaster are good. New, steel studded dry walls are not generally good - they offer little to no support, often serve as vibration/acoustic resonators. (Is there a EMI issue with Steel studs?)
Catch-22 Older maybe bad due to electro-magnetic interference (EMI) issues, particularly old (and new) science buildings as they tend to add additional electrical feeds every which way which causes EMI. Science buildings seem to be bad because there are (albeit physical sciences vs. math or cultural anthropology). Higher ceilings are better (4-5 meters), as electrical and water supply lines which run in the interstitial floor space can cause EMI problems but these drop off quickly with, and the larger room air volume buffers air pressure and temperature.
Wiring: We have to have it, but other than staying away from major building power distribution / supplies waht needs to be considered? And how far from major distributions is reasonable to look at? Question: Twisted power feed cables are ideal, but are they critical?
Wi-fi has been discussed on the microscopy listserv and seems not to be an issue provided you do not mounted the antenna´s on the column.
New Buildings: Built with General construction can be horrible for EMI (lots of electrical supplies for all of 20th century equipment), acoustic (ventilation and steel stud walls), vibrations (lighter cheaper construction methods). BUT if planned for can be controlled and eliminated (?) - however, you (the EM people) have to constantly confirm that construction is being done to spec.
Elevators are not good near EM's, and near = 5 to 8m upto10 to15 meters minimum maybe? I am trying to find out. HOWEVER, there are big problems with a basement level EM Facility which has no elevator access. How do you get the scopes in? Or liquid nitrogen and compressed gas cylinders? Are hydraulic or cable elevators better? (One shaft vs counter weight).
Roads: Lighter the traffic in terms of numbers and vehicle mass is better. Railroads
What have I gotten wrong? What have I ignored? What key factors should be considered?
Campus Politics: Get and keep the biggest research guns and biggest departments happy users, and make the Admin knows you do a good job and keep as many as possible happy.
And so obviously the best location would be a concrete and brick bunker underneath the Arizona desert with no other buildings within 1 kilometer served by a dirt mule path is the only way to go right? Anybody have an old missile silo? Oh but then there´s all those massive steel doors and springs . . .
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 21, 23 -- From edelmare-at-muohio.edu Wed Oct 17 11:01:54 2007 21, 23 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 21, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9HG1ral021760 21, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 17 Oct 2007 11:01:54 -0500 21, 23 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 21, 23 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l9HG1lIF032120 21, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 17 Oct 2007 12:01:47 -0400 21, 23 -- Received: from [192.168.1.23] ([134.53.14.105]) 21, 23 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l9HG1lWK017219 21, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 17 Oct 2007 12:01:47 -0400 21, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 21, 23 -- To: microscopy-at-Microscopy.com 21, 23 -- Date: Wed, 17 Oct 2007 12:01:47 -0400 21, 23 -- MIME-Version: 1.0 21, 23 -- Subject: Where to relocate an EM Facility 21, 23 -- Message-ID: {4715F9AB.19955.18B62727-at-edelmare.muohio.edu} 21, 23 -- Priority: normal 21, 23 -- X-mailer: Pegasus Mail for Windows (4.41) 21, 23 -- Content-type: text/plain; charset=ISO-8859-1 21, 23 -- Content-description: Mail message body 21, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 21, 23 -- Content-Transfer-Encoding: 8bit 21, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id l9HG1ral021760 ==============================End of - Headers==============================
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==============================Original Headers============================== 36, 36 -- From David.Patton-at-uwe.ac.uk Wed Oct 17 11:35:08 2007 36, 36 -- Received: from mailapp04.uwe.ac.uk (mailapp04.uwe.ac.uk [164.11.132.66]) 36, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l9HGZ7VC002066 36, 36 -- for {microscopy-at-Microscopy.com} ; Wed, 17 Oct 2007 11:35:07 -0500 36, 36 -- Received: from (unknown [164.11.132.62]) by mailapp04.uwe.ac.uk with smtp 36, 36 -- id 1b3f_a5c6c856_7cce_11dc_a7d1_00142223915c; 36, 36 -- Wed, 17 Oct 2007 17:33:09 +0100 36, 36 -- Received: from egen-uwe01.campus.ads.uwe.ac.uk 36, 36 -- (egen-uwe01.campus.ads.uwe.ac.uk [164.11.249.121]) 36, 36 -- by mta02.uwe.ac.uk (iPlanet Messaging Server 5.2 HotFix 2.07 (built Jun 24 36, 36 -- 2005)) with SMTP id {0JQ200GNFE2HP6-at-mta02.uwe.ac.uk} for 36, 36 -- microscopy-at-Microscopy.com; Wed, 17 Oct 2007 17:35:05 +0100 (BST) 36, 36 -- Date: Wed, 17 Oct 2007 17:27:36 +0100 36, 36 -- From: David Patton {David.Patton-at-uwe.ac.uk} 36, 36 -- Subject: RE: [Microscopy] Where to relocate an EM Facility 36, 36 -- In-reply-to: {200710171611.l9HGBail028303-at-ns.microscopy.com} 36, 36 -- To: edelmare-at-muohio.edu 36, 36 -- Cc: microscopy-at-Microscopy.com 36, 36 -- Message-id: {F247F674896BE243AD8263C5280E2BDB03157191-at-egen-uwe01} 36, 36 -- MIME-version: 1.0 36, 36 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 36, 36 -- Content-type: text/plain; 36, 36 -- charset="utf-8" 36, 36 -- Content-class: urn:content-classes:message 36, 36 -- Thread-topic: [Microscopy] Where to relocate an EM Facility 36, 36 -- Thread-index: AcgQ2HaFgqZm5FFBQnWkfzt8D+GXwQAAY2zw 36, 36 -- X-MS-Has-Attach: 36, 36 -- X-MS-TNEF-Correlator: 36, 36 -- References: {200710171611.l9HGBail028303-at-ns.microscopy.com} 36, 36 -- X-NAIMIME-Disclaimer: 1 36, 36 -- X-NAIMIME-Modified: 1 36, 36 -- X-NAI-Spam-Score: -1.2 36, 36 -- X-NAI-Spam-Rules: 2 Rules triggered 36, 36 -- BAYES_01=-1.2, HAS_X_HELO=0 36, 36 -- Content-Transfer-Encoding: 8bit 36, 36 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id l9HGZ7VC002066 ==============================End of - Headers==============================
The UI is in the process of consolidating its 4 EMs into one facility so I am painfully aware of the difficulties involved. Our "move" has been 15 years in the making due to, essentially, institutional stupidity. The original facility space developed for the EM Lab (before my time) was a well laid out floor plan: individual bays for up to 5 scopes, large common sample-prep, darkroom, ultramicrotome lab space.....beautiful. All located on the 3rd floor of a steel-framed building-I will spare the gory details of how this happened. Obviously, the space was unusable for EM (any type). The idea of retrofitting each with after-market "fixes" was not a option ($$$) and wouldn't have worked well anyway. The other problem is what I call the "zero-sum space problem:" the UI rarely funds new building with state or private bonds therefore rarely builds new lab space. If you want a new lab someone has to give up that space so you better be good at politics.
My strategy was to locate as many possible space options (non of which were available at the time) and have them all surveyed (EMI, vibrations etc.) along with the 3rd Floor "evil space." The last was done to quell any "Why don't you just use this space" comments. By having site surveys in my pocket when a space did open up I could move quickly with some confidence that the space would work-Upper Administrators like momentum...once in motion it tends to remain in motion. Rooms were not perfect but there were no better alternatives.
Big Acquired Wisdom from this project is that retrofitting lab space is a BAD IDEA-unavoidable maybe........whatta ya gonna do??
Few humble suggestions:
-site survey as many potential sites as you have $$$ for (which you've done). -in addition to basic electrical think about lighting. This seems trivial but we've found that the TEM space needs more controllable lighting (even with CCD cameras) than the SEM labs (obvious but then again, I'm slow) and retrofitting the overhead fluorescents is a pain. NOTE: we had no budget for large-scale renovations such as new walls etc. and even if you do there are problems with new walls in older building (air flow balance for one-see below). -HVAC issues. AC vents, return vents etc. can be a real problem as they seem to always be blowing right on the column! -noise. Some rooms seem quiet when being used as a simple chemistry lab but quite different when there are pumps, chillers, fans etc.
Simple stuff...but like I said, I'm slow. Good luck.
Tom
-----Original Message----- X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu] Sent: Wednesday, October 17, 2007 9:15 AM To: Thomas Williams
I have to move my EM Facility somewhere else on campus because we have out grown our existing location and environmental conditions (Vibration, EMI, and Acoustic) are no longer compatible with EM´s. The chosen site will be renovated for an EM Facility but the question comes as to "Where"?
We have had site survey´s done on two potential locations. One location was horrible (Problems out of spec for vibration, EMI-AC, EMI-DC, and Acoustic). The second was poor (out of spec for EMI-AC for 200 Kv TEM and high-end SEM, with distance issues for EMI-DC). Since we are not going to build a new building just for EM (unreasonable of my administration I know), where do I look for physically compatible space? I would like to solicit comments, discussion and suggestions for developing a search criteria for EM Facility locations.
I am hoping that this criteria maybe of use for more than myself and my facility, as I believe more and more of us will be facing similar issues. "Normal" EM´s are moving into higher and higher capabilities, i.e. 200 - 300 KV TEM´s and ultra low voltage FEG SEM´s are becoming the new "standard", and the microscope environmental specs are getting more and more stringent. Manufacturers do not seem readily jumping to handle/correct/compensate in their engineering designs to deal with these issues and the fact that the "standard" microscope still needs to be installed within an existing research building environment. Note: I am not addressing the ultra-highend bleeding edge scopes (i.e. CS-corrected, monochomatic, FEG, count-the- neutrons-in- the-atoms scopes) - yes, there will always be an in house "support" price to pay for the bleeding edge. I am talking about the fact that the "standard" microscope still needs to be installed within an existing research building environment, and this fact its seems is being ignored my the manufacturers. (Albeit: a recent mailing from one manufacturer does somewhat acknowledge the issues and states their new design does allow for "Reduced installation requirements for acoustic and temperature variations" - the easier environmental issues to correct for and is part of a multi- million dollar scope). Third party add-on anti-vibration or EMI cancellation systems are not going to be as good as something inherently designed into the scope and does allow for the "Its not our microscope that´s the problem its their EMI cancellation system" kind of endless loops. Further more, due to cross-interference of EMI cancellation systems, if you have more than one scope, the scopes need to be space prohibitively far apart (20-25m spacing? Four? Five EM´s?).
Criteria for consideration:
Obviously, there can be no hard and fast rules, and every site needs to ultimately be tested, but the list of potential sites can be reduced or prioritized.
If the options exist: Sandy soil is highly preferred. Clay soils are not good. Are there other soil type considerations? (Wet or flood prone areas are definitely not desired, eh?)
For acoustics and vibration: Older, underground (basements), stone, cinder block, concrete building constructions are good. Interior walls of same or wood studded drywall or plaster are good. New, steel studded dry walls are not generally good - they offer little to no support, often serve as vibration/acoustic resonators. (Is there a EMI issue with Steel studs?)
Catch-22 Older maybe bad due to electro-magnetic interference (EMI) issues, particularly old (and new) science buildings as they tend to add additional electrical feeds every which way which causes EMI. Science buildings seem to be bad because there are (albeit physical sciences vs. math or cultural anthropology). Higher ceilings are better (4-5 meters), as electrical and water supply lines which run in the interstitial floor space can cause EMI problems but these drop off quickly with, and the larger room air volume buffers air pressure and temperature.
Wiring: We have to have it, but other than staying away from major building power distribution / supplies waht needs to be considered? And how far from major distributions is reasonable to look at? Question: Twisted power feed cables are ideal, but are they critical?
Wi-fi has been discussed on the microscopy listserv and seems not to be an issue provided you do not mounted the antenna´s on the column.
New Buildings: Built with General construction can be horrible for EMI (lots of electrical supplies for all of 20th century equipment), acoustic (ventilation and steel stud walls), vibrations (lighter cheaper construction methods). BUT if planned for can be controlled and eliminated (?) - however, you (the EM people) have to constantly confirm that construction is being done to spec.
Elevators are not good near EM's, and near = 5 to 8m upto10 to15 meters minimum maybe? I am trying to find out. HOWEVER, there are big problems with a basement level EM Facility which has no elevator access. How do you get the scopes in? Or liquid nitrogen and compressed gas cylinders? Are hydraulic or cable elevators better? (One shaft vs counter weight).
Roads: Lighter the traffic in terms of numbers and vehicle mass is better. Railroads
What have I gotten wrong? What have I ignored? What key factors should be considered?
Campus Politics: Get and keep the biggest research guns and biggest departments happy users, and make the Admin knows you do a good job and keep as many as possible happy.
And so obviously the best location would be a concrete and brick bunker underneath the Arizona desert with no other buildings within 1 kilometer served by a dirt mule path is the only way to go right? Anybody have an old missile silo? Oh but then there´s all those massive steel doors and springs . . .
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 21, 23 -- From edelmare-at-muohio.edu Wed Oct 17 11:01:54 2007 21, 23 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 21, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9HG1ral021760 21, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 17 Oct 2007 11:01:54 -0500 21, 23 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 21, 23 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l9HG1lIF032120 21, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 17 Oct 2007 12:01:47 -0400 21, 23 -- Received: from [192.168.1.23] ([134.53.14.105]) 21, 23 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l9HG1lWK017219 21, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 17 Oct 2007 12:01:47 -0400 21, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 21, 23 -- To: microscopy-at-Microscopy.com 21, 23 -- Date: Wed, 17 Oct 2007 12:01:47 -0400 21, 23 -- MIME-Version: 1.0 21, 23 -- Subject: Where to relocate an EM Facility 21, 23 -- Message-ID: {4715F9AB.19955.18B62727-at-edelmare.muohio.edu} 21, 23 -- Priority: normal 21, 23 -- X-mailer: Pegasus Mail for Windows (4.41) 21, 23 -- Content-type: text/plain; charset=ISO-8859-1 21, 23 -- Content-description: Mail message body 21, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 21, 23 -- Content-Transfer-Encoding: 8bit 21, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id l9HG1ral021760 ==============================End of - Headers==============================
==============================Original Headers============================== 37, 32 -- From tomw-at-uidaho.edu Wed Oct 17 12:34:34 2007 37, 32 -- Received: from proofadmin.csrv.uidaho.edu (mx2.uidaho.edu [129.101.155.249]) 37, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9HHYYdh015299 37, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Oct 2007 12:34:34 -0500 37, 32 -- Received: from exfe2.its.uidaho.edu (exfe2.its.uidaho.edu [129.101.177.61]) 37, 32 -- by mx2.uidaho.edu (8.13.8/8.13.8) with ESMTP id l9HHYV3Q018775 37, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Oct 2007 10:34:32 -0700 37, 32 -- Received: from EXVS2.its.uidaho.edu ([129.101.177.125]) by exfe2.its.uidaho.edu with Microsoft SMTPSVC(6.0.3790.3959); 37, 32 -- Wed, 17 Oct 2007 10:34:32 -0700 37, 32 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 37, 32 -- Content-class: urn:content-classes:message 37, 32 -- MIME-Version: 1.0 37, 32 -- Content-Type: text/plain; 37, 32 -- charset="iso-8859-1" 37, 32 -- Subject: RE: [Microscopy] Where to relocate an EM Facility 37, 32 -- Date: Wed, 17 Oct 2007 10:33:51 -0700 37, 32 -- Message-ID: {E68EC6D996E21B40BC0017AE100680311CD935-at-EXVS2.its.uidaho.edu} 37, 32 -- In-reply-to: {200710171614.l9HGEfcA030289-at-ns.microscopy.com} 37, 32 -- X-MS-Has-Attach: 37, 32 -- X-MS-TNEF-Correlator: 37, 32 -- Thread-Topic: [Microscopy] Where to relocate an EM Facility 37, 32 -- Thread-Index: AcgQ2NO3NgH+AHntSLy6kXqFMHef+wABRypQ 37, 32 -- References: {200710171614.l9HGEfcA030289-at-ns.microscopy.com} 37, 32 -- From: "Thomas Williams" {tomw-at-uidaho.edu} 37, 32 -- To: {Microscopy-at-microscopy.com} 37, 32 -- X-OriginalArrivalTime: 17 Oct 2007 17:34:32.0309 (UTC) FILETIME=[FA4DEA50:01C810E3] 37, 32 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=4.65.5502:2.3.11,1.2.37,4.0.164 definitions=2007-10-17_06:2007-10-17,2007-10-17,2007-10-17 signatures=0 37, 32 -- X-SpamDetails: rule=notspam policy=default score=0 spamscore=0 ipscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx engine=3.1.0-0708230000 definitions=main-0710170044 37, 32 -- X-SpamBar: 37, 32 -- X-SpamScore: 0 37, 32 -- Content-Transfer-Encoding: 8bit 37, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l9HHYYdh015299 ==============================End of - Headers==============================
} "My strategy was to locate as many possible space options"
And THAT is the question - how do you pick the locations to test?
} "site survey as many potential sites as you have $$$ for"
Comes back to my question: How do "rank" list or limit the locations you get tested? So you can best utilize the limited $$$?
I'm hoping we (the microscopy list community) can come up with a best quess criteria for doing just this.
} My strategy was to locate as many possible space options (non of which } were available at the time) and have them all surveyed (EMI, } vibrations etc.) along with the 3rd Floor "evil space." The last was } done to quell any "Why don't you just use this space" comments. By } having site surveys in my pocket when a space did open up I could move } quickly with some confidence that the space would work-Upper } Administrators like momentum...once in motion it tends to remain in } motion. Rooms were not perfect but there were no better alternatives. }
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 12, 25 -- From edelmare-at-muohio.edu Wed Oct 17 14:14:30 2007 12, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9HJETwB029459 12, 25 -- for {microscopy-at-Microscopy.com} ; Wed, 17 Oct 2007 14:14:29 -0500 12, 25 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 12, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l9HJET0C002440; 12, 25 -- Wed, 17 Oct 2007 15:14:29 -0400 12, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 12, 25 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l9HJETKA031460; 12, 25 -- Wed, 17 Oct 2007 15:14:29 -0400 12, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 12, 25 -- To: "tomw-at-uidaho.edu" {tomw-at-uidaho.edu} 12, 25 -- Date: Wed, 17 Oct 2007 15:14:28 -0400 12, 25 -- MIME-Version: 1.0 12, 25 -- Subject: Re: [Microscopy] RE: Where to relocate an EM Facility 12, 25 -- CC: microscopy-at-Microscopy.com 12, 25 -- Message-ID: {471626D4.6892.196689D9-at-edelmare.muohio.edu} 12, 25 -- Priority: normal 12, 25 -- In-reply-to: {200710171735.l9HHZIZd016478-at-ns.microscopy.com} 12, 25 -- References: {200710171735.l9HHZIZd016478-at-ns.microscopy.com} 12, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 12, 25 -- Content-type: text/plain; charset=US-ASCII 12, 25 -- Content-transfer-encoding: 7BIT 12, 25 -- Content-description: Mail message body 12, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 ==============================End of - Headers==============================
Hi All- I have been asked to do immuno-EM on adherent cells. The PI also provided pellets of the same cells, as controls for the Ab reaction: the cells in the pellets are transfected with the Ag. The rub is that what the PI is REALLY interested in is the interaction of the adherent cells with B cells which have been added to the culture. He is especially interested in looking for the Ag in the fine pseudopod-like projections/connections that form between the 2 cell types. Hence, the cells must remain as an intact monolayer. I've had good luck with the pellets in LRWhite. What can I do about the monolayers? Most of the immuno resins won't polymerize in the presence of air/oxygen. I've tried making an Aclar sandwich, but that was a mess. Should I try an Epon-like resin and then etch the sections? I know that someone out there will have faced this in the past.... -- Leona Cohen-Gould, M.S. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology Core Facility Manager, Optical Microscopy Core Facility Weill Cornell Medical College
Hi Leona It is possible to flat embed your cells in the TC dish. All one needs to do is exclude the air. What I have done is to grow the cells in 35mm dishes. Fix and dehydrate as normal. Fill the dish to over-full and then put the cover on the dish upside-down. If you put the cover on so that one side is down and then you angle the cover down into place (I am not sure that makes sense, let me know if I have to try again) so that there is no bubble under the cover. I have also found that the LRWhite reacts with some TC dish plastic. What has worked is to coat the inside of the TC dish and the top of the cover with sterile, molten 1.5% agar and 0.5% gelatin. I put some in the dish, swirl around until all surfaces are coated, pore out the excess and let dry. The cells do not stick as well, but what I have worked with have been fine. Let me know if you want a formal protocol. David
On Oct 17, 2007, at 1:59 PM, lcgould-at-med.cornell.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi All- } I have been asked to do immuno-EM on adherent cells. The PI also } provided pellets of the same cells, as controls for the Ab reaction: } the cells in the pellets are transfected with the Ag. The rub is } that what the PI is REALLY interested in is the interaction of the } adherent cells with B cells which have been added to the culture. He } is especially interested in looking for the Ag in the fine } pseudopod-like projections/connections that form between the 2 cell } types. Hence, the cells must remain as an intact monolayer. } I've had good luck with the pellets in LRWhite. What can I do about } the monolayers? Most of the immuno resins won't polymerize in the } presence of air/oxygen. I've tried making an Aclar sandwich, but } that was a mess. Should I try an Epon-like resin and then etch the } sections? I know that someone out there will have faced this in the } past.... } -- } Leona Cohen-Gould, M.S. } Sr. Staff Associate in Biochemistry and } Cell & Developmental Biology } Director, Electron Microscopy & Histology Core Facility } Manager, Optical Microscopy Core Facility } Weill Cornell Medical College } } voice (212)746-6146 } fax (212)746-8175 } http://www.cornellcelldevbiology.org } http://www.cornellbiochem.org } } ==============================Original } Headers============================== } 2, 23 -- From lcgould-at-med.cornell.edu Wed Oct 17 15:56:02 2007 } 2, 23 -- Received: from smtp-gw1.med.cornell.edu (smtp- } gw1.med.cornell.edu [140.251.3.74]) } 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id l9HKu2qK011382 } 2, 23 -- for {microscopy-at-microscopy.com} ; Wed, 17 Oct 2007 } 15:56:02 -0500 } 2, 23 -- Received: from mpx2.med.cornell.edu } (pc113142-7.med.cornell.edu [140.251.11.119]) } 2, 23 -- by smtp-gw1.med.cornell.edu (Switch-3.1.8/Switch-3.1.7) } with ESMTP id l9HKtun7012295 } 2, 23 -- for {microscopy-at-microscopy.com} ; Wed, 17 Oct 2007 } 16:56:02 -0400 (EDT) } 2, 23 -- Received: from [140.251.48.23] (mac110773.med.cornell.edu } [140.251.48.23]) } 2, 23 -- by mpx2.med.cornell.edu } 2, 23 -- (Sun Java System Messaging Server 6.1 HotFix 0.11 (built } Jan 28 2005)) } 2, 23 -- with ESMTPA id {0JQ200FPQQ58UQ10-at-mpx2.med.cornell.edu} for } 2, 23 -- microscopy-at-microscopy.com; Wed, 17 Oct 2007 16:55:56 } -0400 (EDT) } 2, 23 -- Date: Wed, 17 Oct 2007 16:55:52 -0400 } 2, 23 -- From: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu} } 2, 23 -- Subject: Microscopy: immuno EM cell monolayers } 2, 23 -- Sender: "Leona Cohen-Gould" {lcgould-at-med.cornell.edu} } 2, 23 -- To: Microscopy Listserver {microscopy-at-microscopy.com} } 2, 23 -- Message-id: {p0623090dc33c22e56ada-at-[140.251.48.23]} } 2, 23 -- MIME-version: 1.0 } 2, 23 -- Content-type: text/plain; charset=us-ascii; format=flowed } 2, 23 -- Content-transfer-encoding: 7BIT } 2, 23 -- X-PMX-Version: 5.3.3.310218, Antispam-Engine: } 2.5.2.313940, Antispam-Data: 2007.10.17.133753 } 2, 23 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%, } Report='__C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, } __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, } __MIME_VERSION 0, __SANE_MSGID 0' } ==============================End of - } Headers============================== }
==============================Original Headers============================== 5, 22 -- From Elliott-at-arizona.edu Wed Oct 17 18:18:20 2007 5, 22 -- Received: from smtpgate.email.arizona.edu (frodo.email.Arizona.EDU [128.196.133.168]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9HNIKen026324 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 17 Oct 2007 18:18:20 -0500 5, 22 -- Received: from frodos_amavis (amavis5.email.arizona.edu [10.0.0.208]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 42F9B27A39F 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 17 Oct 2007 16:18:19 -0700 (MST) 5, 22 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 710A927A2C6 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 17 Oct 2007 16:18:09 -0700 (MST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v752.2) 5, 22 -- In-Reply-To: {200710172059.l9HKxjjU016162-at-ns.microscopy.com} 5, 22 -- References: {200710172059.l9HKxjjU016162-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- Message-Id: {941C30D0-11C5-48CA-9166-FCE6BA846642-at-arizona.edu} 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- From: David Elliott {Elliott-at-arizona.edu} 5, 22 -- Subject: Re: [Microscopy] Microscopy: immuno EM cell monolayers 5, 22 -- Date: Wed, 17 Oct 2007 16:17:59 -0700 5, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 22 -- X-Mailer: Apple Mail (2.752.2) 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mandar.gadre-at-asu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mandar.gadre-at-asu.edu Name: Mandar Gadre
Organization: Arizona State University
Title-Subject: [Filtered] Looking for a used Critical Point Dryer (CPD) Unit
Question: Hello,
We are looking for a used Critical Point Dryer Unit. We would need the size of the chamber to be about 6-8 cm in length and 6-8 cm in inner diameter. If you have a used one which you are willing to part with, we would like to work out a deal.
Looking forward to your reply.
Thanks and Regards,
Mandar Gadre Graduate Student Arizona State University.
I need to replace a broken drive belt on a Reichert Ultracut E ultramicrotome. I last did this, with advice and spare belts from a couple of people in this Group, seven years ago. Well, the way my memory works these days... I found the spare belts I ordered, but I sure can't remember how to get the belt on! I did remember how to get into the thing, and I remember there was some "trick" to getting the belt on around the drive shaft. But I do not remember the "trick". Can anyone help me?
Mahalo! Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 19 -- From tina-at-pbrc.hawaii.edu Wed Oct 17 20:29:58 2007 5, 19 -- Received: from halia.pbrc.hawaii.edu (halia.pbrc.hawaii.edu [128.171.22.7]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9I1Twu0020624 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 17 Oct 2007 20:29:58 -0500 5, 19 -- Received: from halia.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 19 -- by halia.pbrc.hawaii.edu (8.12.11/8.12.11) with ESMTP id l9I1TsJw022489 5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 17 Oct 2007 15:29:55 -1000 (HST) 5, 19 -- Received: from localhost by halia.pbrc.hawaii.edu (8.12.11/8.12.11/Submit) with ESMTP id l9I1Trws022486 5, 19 -- for {Microscopy-at-MSA.Microscopy.Com} ; Wed, 17 Oct 2007 15:29:54 -1000 (HST) 5, 19 -- X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs 5, 19 -- Date: Wed, 17 Oct 2007 15:29:53 -1000 (HST) 5, 19 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 19 -- X-Sender: tina-at-halia 5, 19 -- To: Microscopy Listserver {Microscopy-at-MSA.Microscopy.Com} 5, 19 -- Subject: Replacing belt on Ultracut E 5, 19 -- Message-ID: {Pine.GSO.4.21.0710171526240.22339-100000-at-halia} 5, 19 -- MIME-Version: 1.0 5, 19 -- Content-Type: TEXT/PLAIN; charset=US-ASCII ==============================End of - Headers==============================
Someone else has already suggested doing the polymerization in TC dishes; you can also do cells on coverslips (glass or Thermanox) for flat embedding; the blocks are easier (I think) to retrieve once polymerized, plus you can get away from having to treat the polystyrene dishes to "protect" them from being damaged by the resin. I do resin infiltrations in glass coverslip "Coplin" jars - I'm not sure of their real name, but they are *really* short Coplin-like jars that hold 22 x 22 mm coverslips instead of slides. Smaller coverslips can be done in glass scintillation vials or regular old EM snap-cap vials. A zillion ways to embed: old JB-4 style molds with a bit of Saran wrap, Aclar film (since you already have that), or other transparent cover work very well if you are doing UV polymerization. I used to use the JB-4 chucks as covers when I was doing thermal polymerization in these molds. Another option - several companies sell molds that will work for UV polymerization of coffin blocks if you can work with cells grown on small Thermanox strips, & I know of one company that may make slide-casting molds out of UV-transparent stuff for you if you ask nicely :-) In a pinch, I've used aluminum weighboats with Saran wrap sort of floated on top for bigger samples/coverslips - this uses a lot of resin, but it works. I also tried the Aclar sandwich trick & was not thrilled with the mess that resulted.
Good luck!
Tamara
On Wed, 17 Oct 2007 15:57:23 -0500 lcgould-at-med.cornell.edu wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi All- } I have been asked to do immuno-EM on adherent cells. } The PI also } provided pellets of the same cells, as controls for the } Ab reaction: } the cells in the pellets are transfected with the Ag. } The rub is } that what the PI is REALLY interested in is the } interaction of the } adherent cells with B cells which have been added to the } culture. He } is especially interested in looking for the Ag in the } fine } pseudopod-like projections/connections that form between } the 2 cell } types. Hence, the cells must remain as an intact } monolayer. } I've had good luck with the pellets in LRWhite. What } can I do about } the monolayers? Most of the immuno resins won't } polymerize in the } presence of air/oxygen. I've tried making an Aclar } sandwich, but } that was a mess. Should I try an Epon-like resin and } then etch the } sections? I know that someone out there will have faced } this in the } past.... } -- } Leona Cohen-Gould, M.S. } Sr. Staff Associate in Biochemistry and } Cell & Developmental Biology } Director, Electron Microscopy & Histology Core Facility } Manager, Optical Microscopy Core Facility } Weill Cornell Medical College } } voice (212)746-6146 } fax (212)746-8175 } http://www.cornellcelldevbiology.org } http://www.cornellbiochem.org } } ==============================Original } Headers============================== } 2, 23 -- From lcgould-at-med.cornell.edu Wed Oct 17 } 15:56:02 2007 } 2, 23 -- Received: from smtp-gw1.med.cornell.edu } (smtp-gw1.med.cornell.edu [140.251.3.74]) } 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } with ESMTP id l9HKu2qK011382 } 2, 23 -- for {microscopy-at-microscopy.com} ; Wed, 17 Oct } 2007 15:56:02 -0500 } 2, 23 -- Received: from mpx2.med.cornell.edu } (pc113142-7.med.cornell.edu [140.251.11.119]) } 2, 23 -- by smtp-gw1.med.cornell.edu } (Switch-3.1.8/Switch-3.1.7) with ESMTP id l9HKtun7012295 } 2, 23 -- for {microscopy-at-microscopy.com} ; Wed, 17 Oct } 2007 16:56:02 -0400 (EDT) } 2, 23 -- Received: from [140.251.48.23] } (mac110773.med.cornell.edu [140.251.48.23]) } 2, 23 -- by mpx2.med.cornell.edu } 2, 23 -- (Sun Java System Messaging Server 6.1 HotFix } 0.11 (built Jan 28 2005)) } 2, 23 -- with ESMTPA id } {0JQ200FPQQ58UQ10-at-mpx2.med.cornell.edu} for } 2, 23 -- microscopy-at-microscopy.com; Wed, 17 Oct 2007 } 16:55:56 -0400 (EDT) } 2, 23 -- Date: Wed, 17 Oct 2007 16:55:52 -0400 } 2, 23 -- From: "Leona Cohen-Gould" } {lcgould-at-med.cornell.edu} } 2, 23 -- Subject: Microscopy: immuno EM cell monolayers } 2, 23 -- Sender: "Leona Cohen-Gould" } {lcgould-at-med.cornell.edu} } 2, 23 -- To: Microscopy Listserver } {microscopy-at-microscopy.com} } 2, 23 -- Message-id: } {p0623090dc33c22e56ada-at-[140.251.48.23]} } 2, 23 -- MIME-version: 1.0 } 2, 23 -- Content-type: text/plain; charset=us-ascii; } format=flowed } 2, 23 -- Content-transfer-encoding: 7BIT } 2, 23 -- X-PMX-Version: 5.3.3.310218, Antispam-Engine: } 2.5.2.313940, Antispam-Data: 2007.10.17.133753 } 2, 23 -- X-PerlMx-Spam: Gauge=IIIIIII, Probability=7%, } Report='__C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE } 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, } __MIME_VERSION 0, __SANE_MSGID 0' } ==============================End of - } Headers==============================
Our TN microspec EDS detector has lost vacuum. We're looking for someone who can repair it or help us evaluate whether it's worth the effort. If you know anyone who trouble shoots and repairs detectors this old, please contact me off line. Thanks.
Chris
-- Chris Fleisher Electron Microprobe Lab Coordinator Department of Geology University of Georgia Athens, Ga. 30602 Ph: (706) 542-2419 E-mail: fleisher-at-gly.uga.edu
==============================Original Headers============================== 5, 18 -- From fleisher-at-gly.uga.edu Thu Oct 18 08:02:33 2007 5, 18 -- Received: from puntd1.cc.uga.edu (puntd1.cc.uga.edu [128.192.1.119]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9ID2Xn1006280 5, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Oct 2007 08:02:33 -0500 5, 18 -- Received: from [128.192.40.74] ([128.192.40.74]) 5, 18 -- by puntd1.cc.uga.edu (MOS 3.8.4-GA) 5, 18 -- with ESMTP id IXD00755; 5, 18 -- Thu, 18 Oct 2007 09:02:33 -0400 (EDT) 5, 18 -- Message-ID: {47175A45.1010701-at-gly.uga.edu} 5, 18 -- Date: Thu, 18 Oct 2007 09:06:13 -0400 5, 18 -- From: Chris Fleisher {fleisher-at-gly.uga.edu} 5, 18 -- User-Agent: Mozilla Thunderbird 1.0.2 (Macintosh/20050317) 5, 18 -- X-Accept-Language: en-us, en 5, 18 -- MIME-Version: 1.0 5, 18 -- To: Microscopy-at-microscopy.com 5, 18 -- Subject: Looking for help repairing an EDS detector 5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Thanks for the input. Basement storage closet would be nice but I really need more than one. And what I am finding is that science buildings are NOT the way to go, as science folks like to stuff all the noisey, hot, electrical things in the basement - because who wants to live in the basement anyway?
(I look out my office window and I see a two story basement of a new School of Business, and I'm sitting here wondering how do I get them to give me space for "Science" there. Because I know no Business faculty are going to use the basement area (but why is it two stories tall? 22 feet below ground to the concrete pad?))
Thanks again.
On 18 Oct 2007 at 8:10, Kenneth Livi wrote:
} Richard, } If I were looking around for space, I would look in basement corners. These tend to have low vibration, low EM if away from mechanical rooms and elevators. Maybe there is a store room that can be converted? Of course, you have to invest in the room if you want to give your scope the best chance to perform up to spec or better. We sunk nearly $200k in a room for our CM 300 10 years ago. It was well worth it knowing that we didn't have to fight the environment. Metal studs, or anything that is metal is OK as long as it's not moving. We had acoustic dampening curtains installed that helped cut down the reflected sounds. The noisiest parts of our room are the fans on the computer (we got that down to one) and GIF/EDS devices and the SF6 exhaust (significantly loud enough that you notice it when you turn it off even though the fan is 3 floors above us). The piping, conduits and vents were all routed to the perimeter of the ceiling to make room so the scope emission chamber could be raised. Therefore, we built a "top hat" like ceiling with the center right up to the concrete of the floor above us and the minimum drop ceiling to cover the rest. The curtains were also used to send the A/C intake flow to outside of the "room within a room" and have the cold air flow along the floor and passively rise up with the hot air of the the scope e-rack and computer rack (the two exhaust vents are directly above these areas). We also installed a track of red incandescent lights pointing at choice locations with a remote (long cable) dimmer. The "mood" lighting is great for showing off the scope to visitors. It looks like we really do something here! } Ciao for now, } Ken } } Kenneth Livi, Ph.D. } High-Resolution Analytical Electron Microbeam Facility } 3400 N Charles Street } Department of Earth and Planetary Sciences } Johns Hopkins University } Baltimore, Maryland 21218 USA } } } }
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 10, 25 -- From edelmare-at-muohio.edu Thu Oct 18 08:07:10 2007 10, 25 -- Received: from mulnx11.mcs.muohio.edu (mulnx11.mcs.muohio.edu [134.53.6.66]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9ID798s011152 10, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Oct 2007 08:07:10 -0500 10, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 10, 25 -- by mulnx11.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l9ID78ZM014896; 10, 25 -- Thu, 18 Oct 2007 09:07:08 -0400 10, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 10, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id l9ID78Mo030155; 10, 25 -- Thu, 18 Oct 2007 09:07:08 -0400 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 10, 25 -- To: Kenneth Livi {klivi-at-jhu.edu} , 10, 25 -- "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 25 -- Date: Thu, 18 Oct 2007 09:07:08 -0400 10, 25 -- MIME-Version: 1.0 10, 25 -- Subject: Re: [Microscopy] Where to relocate an EM Facility 10, 25 -- Message-ID: {4717223C.2629.1D3C6788-at-edelmare.muohio.edu} 10, 25 -- Priority: normal 10, 25 -- In-reply-to: {365F712F-EC1C-4204-A695-654C84ED5202-at-jhu.edu} 10, 25 -- References: {200710171609.l9HG9T5s027101-at-ns.microscopy.com} , {365F712F-EC1C-4204-A695-654C84ED5202-at-jhu.edu} 10, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 10, 25 -- Content-type: text/plain; charset=US-ASCII 10, 25 -- Content-transfer-encoding: 7BIT 10, 25 -- Content-description: Mail message body 10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.66 ==============================End of - Headers==============================
I'm a little late in joining this discussion; please forgive me if this has already been mentioned. We also like thermanox round coverslips for growing cells since they do not react with any media or solvent that is generally used for EM. We embed them in wheaton snap caps, which are also impervious to embedding media and the smaller coverslips (aobut 11mm) fit well in them. If polymerization is to be done at 60C, a 1000 ml beaker of dry ice can be included in the polymerization oven to insure a co2 atmosphere in which the media will polymerize with no problems. I expect something similar could be done with UV polymerization, but I have not tried sincce we do this in a freeze-substitution apparatus under N2 atomoshpere.
Good luck,
Doug
==============================Original Headers============================== 5, 26 -- From drk-at-shcc.org Thu Oct 18 09:45:17 2007 5, 26 -- Received: from mail.shcc.org (shcc.org [64.213.211.200]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9IEjHOl000718 5, 26 -- for {microscopy-at-microscopy.com} ; Thu, 18 Oct 2007 09:45:17 -0500 5, 26 -- Received: from por-ex-ch01.research.shcc.org (64.213.211.212) by mail.shcc.org 5, 26 -- (64.213.211.200) with Microsoft SMTP Server (TLS) id 8.0.730.1; Thu, 18 Oct 5, 26 -- 2007 07:45:20 -0700 5, 26 -- Received: from por-ex-mb01.research.shcc.org ([64.213.211.102]) by 5, 26 -- por-ex-ch01.research.shcc.org ([64.213.211.212]) with mapi; Thu, 18 Oct 2007 5, 26 -- 07:47:23 -0700 5, 26 -- From: Doug Keene {drk-at-shcc.org} 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 26 -- Date: Thu, 18 Oct 2007 07:47:23 -0700 5, 26 -- Subject: LR White embedding of cultures 5, 26 -- Thread-Topic: LR White embedding of cultures 5, 26 -- Thread-Index: AQHIEZXKmw7/rWtIrEmVsETIsMWlBA== 5, 26 -- Message-ID: {145D57516E39804A832D96C2EC1C553F0F7A6E25-at-por-ex-mb01.research.shcc.org} 5, 26 -- Accept-Language: en-US 5, 26 -- Content-Language: en-US 5, 26 -- X-MS-Has-Attach: 5, 26 -- X-MS-TNEF-Correlator: 5, 26 -- acceptlanguage: en-US 5, 26 -- Content-Type: text/plain; charset="us-ascii" 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Transfer-Encoding: 8bit 5, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l9IEjHOl000718 ==============================End of - Headers==============================
Energy Beam Sciences would be able to help you with a new model, as well as used.
I will contact you off-line on this.
Mike Dufraine
mandar.gadre-at-asu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mandar.gadre-at-asu.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mandar.gadre-at-asu.edu } Name: Mandar Gadre } } Organization: Arizona State University } } Title-Subject: [Filtered] Looking for a used Critical Point Dryer (CPD) Unit } } Question: Hello, } } We are looking for a used Critical Point Dryer Unit. We would need the size of the chamber to be about 6-8 cm in length and 6-8 cm in inner diameter. } If you have a used one which you are willing to part with, we would like to work out a deal. } } Looking forward to your reply. } } Thanks and Regards, } } Mandar Gadre } Graduate Student } Arizona State University. } } } } Login Host: 149.169.225.26 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 12, 11 -- From zaluzec-at-microscopy.com Wed Oct 17 18:38:55 2007 } 12, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 12, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9HNcs9h006324 } 12, 11 -- for {microscopy-at-microscopy.com} ; Wed, 17 Oct 2007 18:38:55 -0500 } 12, 11 -- Mime-Version: 1.0 } 12, 11 -- Message-Id: {p06240801c33c4d7f03ac-at-[206.69.208.22]} } 12, 11 -- Date: Wed, 17 Oct 2007 18:38:53 -0500 } 12, 11 -- To: microscopy-at-microscopy.com } 12, 11 -- From: mandar.gadre-at-asu.edu (by way of MicroscopyListserver) } 12, 11 -- Subject: viaWWW: Looking for a used Critical Point Dryer (CPD) Unit } 12, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
-- Michael F. Dufraine EM - Product Manager TEL 800-992-9037 X340 MDufraine-at-ebsciences.com www.ebsciences.com
==============================Original Headers============================== 8, 27 -- From mdufraine-at-ebsciences.com Thu Oct 18 10:12:32 2007 8, 27 -- Received: from mx-outbound01.easydns.com (mailout.easydns.com [205.210.42.54]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9IFCWWC013603 8, 27 -- for {microscopy-at-microscopy.com} ; Thu, 18 Oct 2007 10:12:32 -0500 8, 27 -- Received: from mail.ebsciences.com (unknown [69.182.224.2]) 8, 27 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 8, 27 -- (No client certificate requested) 8, 27 -- by mx-outbound01.easydns.com (Postfix) with ESMTP id 593888323; 8, 27 -- Thu, 18 Oct 2007 11:12:30 -0400 (EDT) 8, 27 -- Received: from [10.10.0.190] 8, 27 -- by mail.ebsciences.com with esmtpsa (TLSv1:AES256-SHA:256) 8, 27 -- (Exim 4.67) 8, 27 -- (envelope-from {mdufraine-at-ebsciences.com} ) 8, 27 -- id 1IiX30-00050d-Cc; Thu, 18 Oct 2007 11:12:30 -0400 8, 27 -- Message-ID: {471777DD.7000905-at-ebsciences.com} 8, 27 -- Date: Thu, 18 Oct 2007 11:12:29 -0400 8, 27 -- From: Mike Dufraine {mdufraine-at-ebsciences.com} 8, 27 -- Organization: Energy Beam Sciences 8, 27 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 8, 27 -- MIME-Version: 1.0 8, 27 -- To: mandar.gadre-at-asu.edu, microscopy-at-microscopy.com 8, 27 -- Subject: Re: [Microscopy] viaWWW: Looking for a used Critical Point Dryer 8, 27 -- (CPD) Unit 8, 27 -- References: {200710172339.l9HNdSUA007388-at-ns.microscopy.com} 8, 27 -- In-Reply-To: {200710172339.l9HNdSUA007388-at-ns.microscopy.com} 8, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
As one who has to do flat embedding every once in a while - yes, I so admit there is more to the world of viral EM than negative staining - I have followed this line with interest. Unfortunately we do not see all of the responses to Leona's quiery. I'm comfortable with how we do things using routine, organic based resins. But LR White has been a challenge over the years.
Doug, are you referring to the wheaton snap cap closures with the septa or the snap cap glass vials. If you are using the septa caps - a neat idea by the way - how are you covering the sample for LR White? Are you filling them and using aclar, do y ou have another sealer?
Paul
==============================Original Headers============================== 7, 21 -- From paul_hazelton-at-umanitoba.ca Thu Oct 18 10:23:39 2007 7, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9IFNd0b025727 7, 21 -- for {microscopy-at-microscopy.com} ; Thu, 18 Oct 2007 10:23:39 -0500 7, 21 -- Received: from umanitoba.ca (net40.medmb.umanitoba.ca [140.193.11.160]) 7, 21 -- (authenticated bits=0) 7, 21 -- by electra.cc.umanitoba.ca (8.13.0/8.13.0) with ESMTP id l9IFNc0Q021581; 7, 21 -- Thu, 18 Oct 2007 10:23:38 -0500 (CDT) 7, 21 -- Message-ID: {47177A9C.5040803-at-umanitoba.ca} 7, 21 -- Date: Thu, 18 Oct 2007 10:24:12 -0500 7, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 7, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.4) Gecko/20030624 Netscape/7.1 (ax) 7, 21 -- X-Accept-Language: en-us, en 7, 21 -- MIME-Version: 1.0 7, 21 -- To: drk-at-shcc.org 7, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com} 7, 21 -- Subject: Re: [Microscopy] LR White embedding of cultures 7, 21 -- References: {200710181447.l9IElJfX004017-at-ns.microscopy.com} 7, 21 -- In-Reply-To: {200710181447.l9IElJfX004017-at-ns.microscopy.com} 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
It was several years ago, but I Jim Nicolino satisfactorily rebuilt one of my LN2 EDS detectors. No vested interest. Latest web site I have: http://advancedanalysistech.com/index2.html
Woody White BWXT Services
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Hi All,
Our TN microspec EDS detector has lost vacuum. We're looking for someone who can repair it or help us evaluate whether it's worth the effort. If you know anyone who trouble shoots and repairs detectors this old, please contact me off line. Thanks.
Chris
-- Chris Fleisher Electron Microprobe Lab Coordinator Department of Geology University of Georgia Athens, Ga. 30602 Ph: (706) 542-2419 E-mail: fleisher-at-gly.uga.edu
==============================Original Headers============================== 5, 18 -- From fleisher-at-gly.uga.edu Thu Oct 18 08:02:33 2007 5, 18 -- Received: from puntd1.cc.uga.edu (puntd1.cc.uga.edu [128.192.1.119]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9ID2Xn1006280 5, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Oct 2007 08:02:33 -0500 5, 18 -- Received: from [128.192.40.74] ([128.192.40.74]) 5, 18 -- by puntd1.cc.uga.edu (MOS 3.8.4-GA) 5, 18 -- with ESMTP id IXD00755; 5, 18 -- Thu, 18 Oct 2007 09:02:33 -0400 (EDT) 5, 18 -- Message-ID: {47175A45.1010701-at-gly.uga.edu} 5, 18 -- Date: Thu, 18 Oct 2007 09:06:13 -0400 5, 18 -- From: Chris Fleisher {fleisher-at-gly.uga.edu} 5, 18 -- User-Agent: Mozilla Thunderbird 1.0.2 (Macintosh/20050317) 5, 18 -- X-Accept-Language: en-us, en 5, 18 -- MIME-Version: 1.0 5, 18 -- To: Microscopy-at-microscopy.com 5, 18 -- Subject: Looking for help repairing an EDS detector 5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 16, 29 -- From nwwhite-at-bwxt.com Thu Oct 18 12:47:20 2007 16, 29 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 16, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9IHlKB9009269 16, 29 -- for {microscopy-at-msa.microscopy.com} ; Thu, 18 Oct 2007 12:47:20 -0500 16, 29 -- Received: from ([131.184.13.224]) 16, 29 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.6408680; 16, 29 -- Thu, 18 Oct 2007 13:46:53 -0400 16, 29 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 16, 29 -- Thu, 18 Oct 2007 13:46:53 -0400 16, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 29 -- Content-class: urn:content-classes:message 16, 29 -- MIME-Version: 1.0 16, 29 -- Content-Type: text/plain; 16, 29 -- charset="us-ascii" 16, 29 -- Subject: RE: [Microscopy] Looking for help repairing an EDS detector 16, 29 -- Date: Thu, 18 Oct 2007 13:46:53 -0400 16, 29 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB7F87A00-at-BWXSPO01.BWXS.BWXTECH.NET} 16, 29 -- In-Reply-To: {200710181303.l9ID3MJr007031-at-ns.microscopy.com} 16, 29 -- X-MS-Has-Attach: 16, 29 -- X-MS-TNEF-Correlator: 16, 29 -- Thread-Topic: [Microscopy] Looking for help repairing an EDS detector 16, 29 -- Thread-Index: AcgRh3X897AtWOTtQbijDPf23bclSAAJsJ6w 16, 29 -- References: {200710181303.l9ID3MJr007031-at-ns.microscopy.com} 16, 29 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 16, 29 -- To: {fleisher-at-gly.uga.edu} , 16, 29 -- "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 16, 29 -- X-OriginalArrivalTime: 18 Oct 2007 17:46:53.0614 (UTC) FILETIME=[DE91D4E0:01C811AE] 16, 29 -- Content-Transfer-Encoding: 8bit 16, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l9IHlKB9009269 ==============================End of - Headers==============================
} His name is Jim Nicolino - he specializes in detector repair and I } personally can recommend him!
PulseTor/AAT 1816 St. Johns Bluff Road Suite 305 Jacksonville, Florida 32246 904.646.3069 FAX 904.646.3131 JNicolino-at-comcast.net
Peter
} ---------------------------------------------------------------------------- } } Hi All, } } Our TN microspec EDS detector has lost vacuum. We're looking for } someone who can repair it or help us evaluate whether it's worth the } effort. If you know anyone who trouble shoots and repairs detectors } this old, please contact me off line. Thanks. } } Chris } } -- } Chris Fleisher } Electron Microprobe Lab Coordinator } Department of Geology } University of Georgia } Athens, Ga. 30602 } Ph: (706) 542-2419 E-mail: fleisher-at-gly.uga.edu
-- Peter Ingram Sr. Physicist Adj. Professor of Pathology, Duke University Medical Center Box 90319 LaSalle Street Extension DURHAM NC USA 27708-0319
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cynthia.page-at-colorado.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Question: Hi I am wondering if any one has a good source for Dumont Inox N5 self closing Tweezer. We buy them by the dozen and our last order contained 5 out of 12 tweezers with tips either bent, twisted or just too loose. I am so surprised to have this issue with these fine tweezers I would think they would inspect them before sending. Has anyone experienced this?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cayala-at-lifespan.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: cayala-at-lifespan.org Name: Carol Ayala
Organization: Rhode Island Hospital
Title-Subject: [Filtered] Re: Immuno EM cell monolayers
Question: Hello,
I thought Iíd add my two cents as well in regards to embedding adherent cells for immuno-EM.
I have found that cells grown specifically on Permanox plastic slides (Lab-Tek Chamber slides) can easily be embedded in LR White resin. Once the slide has been processed into LR White, it can be scored and broken into 2 pieces. I use aluminum weighing dishes as my embedding molds. (Low form, aluminum, fluted, weighing dish, approx.2 1/4" wide by 1/2" deep). Put approximately 10-12ml LR White into dish and place the half slide,- Cell Side Down!, on top of the liquid, it will float. Carefully place a second weigh dish down onto the surface of the resin. The second weigh dish should seat neatly inside the first dish (and is actually floating on the liquid). The meniscus of the liquid should rise up between the dishes and just be visible up at the lip. Place these in the oven for polymerization,. These overlapping dishes block out the air so there is no problem with polymerization. Next day carefully peel off aluminum dishes and, using a jewelers saw, cut right along the edges of the slide. I smooth the edges of the slide with sandpaper and score the interface between slide and resin. Then starting at the corners, lightly wedging a razor blade between the slide and resin you can easily separate the two layers. I have done this successfully many, many times.
Additionally I should mention that I have noticed a difference in the temperature at which LR White will polymerize. (And my oven is very stable). The most recent, very fresh supply I have is able to polymerize at 48 C. While tests in the past have shown that the lowest temp at which it would polymerize was 52 C. I donít know if this varies from lot to lot, or if it is a function of the freshness of the resin.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mike.net.mo-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mike.net.mo-at-gmail.com Name: fanxiaoming
Title-Subject: [Filtered] Fxm Need help on Confocal FV1000 with two-photon laser
Question: We used two-photon laser as emission light source,but we only observe the image by DAPI staining(light wavelengh:710nm),when we increased the wavelengh and the light power of the two -photon laser we can't get the imgae by GFP stainning(light wavelengh: {910nm),I don't know which reason caused this problem,could you give me some advice ? Thanks!
This Question was submitted to Ask-A-Microscopist by (fara.sudlow-at-stjude.org) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, October 17, 2007 at 13:40:20 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both fara.sudlow-at-stjude.org as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: fara.sudlow-at-stjude.org Name: fara sudlow
Organization: SJCRH
Education: Undergraduate College
Location: Memphis, TN USA
Title: 2-D analysis software
Question: I was wondering if you had any recommendations for software that will perform some in-depth 2-D analysis. I am interested in free and licensed software and please include any pros and cons associated with each that you may know.
I am working for Sharon Frase at St. Jude Children's Research Hospital and we are researching different types of software applications that would fit our current needs and allow us to expand more in the future. The 2D analysis would include length, width, area, threshholding, particle counting... Any information that you might have on different programs as they relate to TEM would be much appreciated, like how do they deal with a large grayscale and are there parameters to determine what actually is a particle, etc. Also, if you have any ideas on 3D reconstruction, we would greatly appreciate that information as well.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.msa.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both camiller-at-anatomy.iupui.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: camiller-at-anatomy.iupui.edu Name: Caroline Miller
Organization: Indiana Microscopy Society
Title-Subject: [Filtered] Fall Meeting
Question: The Indiana Microscopy Society is having a fall meeting and social on November 7, 2007 at the Clarian Pathology Laboratory Building, Indianapolis, IN from 4:00-8:00 PM. There is no charge for the meeting or dinner if a paid 2007-2008 member. We have two quest speakers, Tuli Mukhopadhyay, from IU, Bloomington, "How to Assemble a Virus:Microscopes Required (Cryo High Resolution EM) and James F. Leary from Purdue, "High Speed Interactive Molecular Imaging with Laser Opto-injection of Macromolecules and Cell Sorting" For more information check out the INMS website, www.indianamicroscopy.org
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both steven.mcdaniel-at-ucr.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: steven.mcdaniel-at-ucr.edu Name: Stephen McDaniel
Question: Does anyone one on the list have experience with the EFFA Multiple Grid Staining Device? I haven't found any references about its use.
} From the EMS website
"Simple and easy to use for most known EM staining techniques. Filtered, multiple step staining of up to 24 specimen grids in a carbon dioxide free environment. All plastic construction and it is resistant to all EM staining chemicals."
==============================Original Headers============================== 8, 11 -- From zaluzec-at-microscopy.com Sat Oct 20 11:00:21 2007 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9KG0Jar001547 8, 11 -- for {microscopy-at-microscopy.com} ; Sat, 20 Oct 2007 11:00:20 -0500 8, 11 -- Mime-Version: 1.0 8, 11 -- Message-Id: {p06240800c33fd6862561-at-[206.69.208.22]} 8, 11 -- Date: Sat, 20 Oct 2007 11:00:17 -0500 8, 11 -- To: microscopy-at-microscopy.com 8, 11 -- From: steven.mcdaniel-at-ucr.edu (by way of MicroscopyListserver) 8, 11 -- Subject: viaWWW: EFFA Multiple Grid Staining Device 8, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
From coordinatormartinprak-at-yahoo.de Mon Oct 22 13:37:58 2007 Return-Path: {coordinatormartinprak-at-yahoo.de} Received: from mail.pvcsd.org (mail.ccsd.k12.ny.us [209.94.106.5] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9MIbqrG007467; Mon, 22 Oct 2007 13:37:54 -0500 Received: from mail.pvcsd.org (localhost [127.0.0.1]) by mail.pvcsd.org (8.12.11/8.12.11/Debian-5) with ESMTP id l9MIZMlv032697; Mon, 22 Oct 2007 14:35:24 -0400 Received: (from www-data-at-localhost) by mail.pvcsd.org (8.12.11/8.12.11/Debian-5) id l9MIY0w6032632; Mon, 22 Oct 2007 14:34:00 -0400 X-Authentication-Warning: mail.pvcsd.org: www-data set sender to coordinatormartinprak-at-yahoo.de using -f Received: from 80.255.59.242 ([80.255.59.242]) by mail.pvcsd.org (IMP) with HTTP for {ggutterman-at-localhost} ; Mon, 22 Oct 2007 14:33:59 -0400 Message-ID: {1193078039.471ced1757c73-at-mail.pvcsd.org}
Hello All,
I am looking for a copy of the circuit schematic for a GW Electronics (BSE) "System 47".
I thought I had one long ago, but it is certainly not included in my copy of the manual. I would really appreciate locating a copy that can be scanned and emailed to me.
Thanks,
Woody White BWXT Services Lynchburg, VA nwwhite-at-bwxt.com
==============================Original Headers============================== 7, 26 -- From nwwhite-at-bwxt.com Mon Oct 22 13:58:29 2007 7, 26 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9MIwTVW008055 7, 26 -- for {microscopy-at-msa.microscopy.com} ; Mon, 22 Oct 2007 13:58:29 -0500 7, 26 -- Received: from ([131.184.13.224]) 7, 26 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.6448990; 7, 26 -- Mon, 22 Oct 2007 14:58:07 -0400 7, 26 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.1830); 7, 26 -- Mon, 22 Oct 2007 14:58:07 -0400 7, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 26 -- Content-class: urn:content-classes:message 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Type: text/plain; 7, 26 -- charset="us-ascii" 7, 26 -- Subject: BSE Schematic? 7, 26 -- Date: Mon, 22 Oct 2007 14:58:07 -0400 7, 26 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB7F87A06-at-BWXSPO01.BWXS.BWXTECH.NET} 7, 26 -- X-MS-Has-Attach: 7, 26 -- X-MS-TNEF-Correlator: 7, 26 -- Thread-Topic: BSE Schematic? 7, 26 -- Thread-Index: AcgU3XuKnKtHPtyKRJKOLECYdC2OsA== 7, 26 -- From: "White, Woody N." {NWWhite-at-bwxt.com} 7, 26 -- To: "MicroscopyListServer" {Microscopy-at-msa.microscopy.com} 7, 26 -- X-OriginalArrivalTime: 22 Oct 2007 18:58:07.0653 (UTC) FILETIME=[7BBF6D50:01C814DD] 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l9MIwTVW008055 ==============================End of - Headers==============================
I would like to wake up the list (is it hibernating?) with the following issue. I prepare Epon for 15 years and it was always clear, like a good old Johnnie Walker (and almost as toxic :-D). Today, after I added BDMA and I carefully mixed as usual, I found it full of small dirt, like precipitates. At the beginning I thought I mixed too strongly (sometimes I cannot control my strength ;-)) and these were small bubbles so I let it calm down, but to no avail. These are definitely NOT small bubbles. It never happened before and I wonder what I should do.
- Do somebody have had this problem before? - Can I use this mixture? - What went wrong? There is no expiry date on none of the products but they are not older than y few years (stored at RT). I am afraid that, if I prepare it again with the same products, I will get the same result.
Best regards, Stephane
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==============================Original Headers============================== 6, 21 -- From nizets2-at-yahoo.com Tue Oct 23 02:51:02 2007 6, 21 -- Received: from web37411.mail.mud.yahoo.com (web37411.mail.mud.yahoo.com [209.191.91.143]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l9N7p2Nc002784 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 23 Oct 2007 02:51:02 -0500 6, 21 -- Received: (qmail 50406 invoked by uid 60001); 23 Oct 2007 07:51:02 -0000 6, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 21 -- s=s1024; d=yahoo.com; 6, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 6, 21 -- b=wHtoV6KVC+u+Rq+OUVGi+dEF5VREX62ygJx3z0qahiObZuZ2plPnDTgXhCxOIoYK/5hg29a/Xg6icS05QSvQqPO14mWFIq2+JEGGEXnS1AxD9TzzJydzkqoOoKPcz9LU1dPQtSSuRRaLksBH1QKE8hN6RQBXyzCKLFm/2XYxwQg=; 6, 21 -- X-YMail-OSG: c_hdOF0VM1moIen1xpCIj4E5MwiUbNj1_f0GpeAZTHFWGFQgaSqY_z3PZhg0QWHInTUQyHrpg52WckXpNE9OzuKqI34p.utHrMxoxPEC73Q85QngUMg- 6, 21 -- Received: from [80.122.101.102] by web37411.mail.mud.yahoo.com via HTTP; Tue, 23 Oct 2007 00:51:01 PDT 6, 21 -- X-Mailer: YahooMailRC/814.06 YahooMailWebService/0.7.134.12 6, 21 -- Date: Tue, 23 Oct 2007 00:51:01 -0700 (PDT) 6, 21 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 21 -- Subject: dirty Epon 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: text/plain; charset=us-ascii 6, 21 -- Message-ID: {962563.49505.qm-at-web37411.mail.mud.yahoo.com} 6, 21 -- Content-Transfer-Encoding: 8bit 6, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l9N7p2Nc002784 ==============================End of - Headers==============================
Recently I started using a new Hitachi TM-1000 desktop SEM.
It is wonderfully easy to use - taking about 5 minutes to switch on, load samples and start running.
My impression is that this SEM uses a relatively low energy electron beam that offers:
(i) relatively low-resolution (good to around 1,000 to 2,000 x magnification, which is enough for many purposes),
(ii) very quick sample inspection (for macro-materials such as seeds, other plant parts, insects, and so on),
(iii) no need for cooling, a high vacuum, and carbon or other coating of samples.
With this kind of set up, what are the likely problems that new users should be alert to?
I do not have any other experience of SEM, apart from a brief period of undergraduate training with a 1980s era, high-resolution, liquid nitrogen-cooled SEM (used for thin-section, freeze-fracture, and ultrastructure studies).
The instruction book tells us to avoid sticky fluids that will attach to the detector and destroy it. Does this mean we cannot use a ballpoint pen to mark areas of the tape with ink? Can we use an alcohol-based ink (e.g. marker pen) for this purpose?
What kind of tape is best to use on stubs to hold samples?
The microscopy tapes we have are either too strong and leave a mess of adhesive on the stub, or produce a grainy background that makes it difficult to distinguish the fine feathery structures on the foot of a small insect.
What kinds of sample treatments can be recommended for an SEM machine of this sort?
It seems that smaller samples (i.e. pollen) quickly develop a fuzzy halo which obscures detail - this may be an effect of electric charge building up (I guess). Or is it a result of working at the limit of the working distance of the electron focusing system?
What elementary or other textbooks on (low-resolution) SEM can be recommended?
Are the kinds of questions I am asking already answered in such books?
Peter Matthews
(Ethnobotany & Prehistory, National Museum of Ethnology, Osaka, Japan).
==============================Original Headers============================== 21, 24 -- From pjm=microscopy=microscopy.com=sntnljft-at-gol.com Tue Oct 23 06:31:09 2007 21, 24 -- Received: from smtp01.dentaku.gol.com (smtp01.dentaku.gol.com [203.216.5.71]) 21, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9NBV8Sp021673 21, 24 -- for {microscopy-at-microscopy.com} ; Tue, 23 Oct 2007 06:31:09 -0500 21, 24 -- Received: from www-data by smtp01.dentaku.gol.com with local (Dentaku) 21, 24 -- id 1IkHyV-0006RB-F1; Tue, 23 Oct 2007 20:31:07 +0900 21, 24 -- To: microscopy-at-microscopy.com 21, 24 -- Subject: Advice needed for using Hitachi TM-1000 desktop SEM 21, 24 -- Received: from 220.145.14.114 (auth. user pjm-at-pp.mail.gol.com) 21, 24 -- by www.webmail.gol.com with HTTP; Tue, 23 Oct 2007 11:31:07 +0000 21, 24 -- X-IlohaMail-Blah: pjm-at-gol.com 21, 24 -- X-IlohaMail-Method: mail() [mem] 21, 24 -- X-IlohaMail-Dummy: moo 21, 24 -- X-Mailer: IlohaMail/0.8.14 (On: www.webmail.gol.com) 21, 24 -- Message-ID: {zSKJ4sD0.1193139067.4503810.pjm-at-gol.com} 21, 24 -- In-Reply-To: {200710230755.l9N7t82D008207-at-ns.microscopy.com} 21, 24 -- From: "Peter Matthews" {pjm-at-gol.com} 21, 24 -- Bounce-To: "Peter Matthews" {pjm-at-gol.com} 21, 24 -- Errors-To: "Peter Matthews" {pjm-at-gol.com} 21, 24 -- CC: pjm-at-gol.com 21, 24 -- MIME-Version: 1.0 21, 24 -- Content-Type: text/plain; charset="us-ascii" 21, 24 -- Date: Tue, 23 Oct 2007 20:31:07 +0900 21, 24 -- X-Abuse-Complaints: abuse-at-gol.com ==============================End of - Headers==============================
You are correct, the TM-1000 is wonderfully easy to use! I'd like to add it was high schooler tested and approved! Over the past summer I had the opportunity to introduce high school students to SEM with the TM-1000 in the classroom. Over 70 students got hands on time with the TM-1000 and the feedback was it was fun, exciting and a "window to another world"!!!!
The TM-1000 utilizes a tungsten filament source operated at 15kV to obtain a resolution of ~30nm (from what I remember) and magnifications up to 10,000X. There is a solid state backscattered electron detector and a charge up reduction mode which reduces sample charging for insulating samples. All of these specs made imaging beef jerky, nail polish, coffee beans, peanuts, broccoli, a pencil eraser and whatever else the students chose really simple.
We experienced no problems with the TM-1000 over the entire term of the course. Just make sure the sample height does not exceed the maximum height on the height gauge and you will not have to worry about getting sticky fluids on the pole piece or detector. (If you open the specimen chamber door and crouch down and peak up into the sample chamber you can see the four-quadrant electron detector at the bottom of the pole piece, this is what you have to be careful not to damage!) So mark away with the ball point pen.
As for tapes....wee just used standard double sided carbon tape and quarters, nickles and pennies as the sample pucks. I guess you could try a 3M double sided tape which is easier to remove than the carbon tape. Although, a little acetone gets any adhesive off relatively easily.
The fuzzy halo you mention......it may be you do not have the TM-1000 in the charge-up reduction mode which is found in one of the drop down menus. Any sample treatments you perform on standard SEM samples are fair game for the TM-1000, but make your life simple, don't chance a heavy gold coating or laborious critical point drying. Just stick it on the stub and image.
Well, I can go on about how much fun students had with the TM-1000 but I have to get some work done. If you'd like to see the over 400 micrographs the students acquired during the course from magnification of 50X - 10,000X of a variety of samples then visit
and if you want to see presentations the students created from the micrograph data visit
http://duketip.nano2.googlepages.com
Hope some of this info helps. Good luck with the TM-1000.
As a disclaimer I do not work for or sell the TM-1000, just a microscopist who had a really great time allowing students to discover the micro and nano-scale with this SEM. Every high school should have a TM-1000. Or at least we should load up a few RVs with 2-3 of them and tour different schools introducing students of all ages to not only microscopy but science and technology!
Donovan
Donovan N. Leonard Visiting Research Assistant Professor Dept. Physics & Astronomy Appalachian State University 231 CAP Building 525 Rivers St. Boone, NC 28608
==============================Original Headers============================== 14, 27 -- From donovan.leonard-at-gmail.com Tue Oct 23 08:35:35 2007 14, 27 -- Received: from nz-out-0506.google.com (nz-out-0506.google.com [64.233.162.239]) 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9NDZZZc005546 14, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 23 Oct 2007 08:35:35 -0500 14, 27 -- Received: by nz-out-0506.google.com with SMTP id o37so764343nzf 14, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 23 Oct 2007 06:35:35 -0700 (PDT) 14, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 27 -- d=gmail.com; s=beta; 14, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 14, 27 -- bh=xZH6J/2npZ8+9wrdaaDUxLHf4y/h6acGX2iOXzxMrv0=; 14, 27 -- b=ZDw8aUB1X5Xm+ywiomi9UIGsfJB+6SI3RyBg78tKK7gEAzw/cKM/pJi0PI/RX6O947PsBfPFgkosIultQks0/vDMF4zRF/tnHNMwyU2ZOsz25myWsOIM3lF1U4pg1fojfqShO2iTNGJR9zpPgFRqZlyHMAi/C/yVI2wvLUNEun0= 14, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 14, 27 -- d=gmail.com; s=beta; 14, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 14, 27 -- b=j/O3uq3tcjoJivOJ5LwSkIz2FLJWLpxqc5e3LrbmlR73LtQj6y8Xm9Rj35RWViUW80vD1THjtMYs8NNLqgtw5eUsVPAxvz498sMt0rdtlSNhKiKS+tRiZvoEdeD7REaQ7S0pIP7oG4zAxD0/YSEnQqAg8ohqyK+vBsNBplbHvZg= 14, 27 -- Received: by 10.115.47.1 with SMTP id z1mr3124873waj.1193146532330; 14, 27 -- Tue, 23 Oct 2007 06:35:32 -0700 (PDT) 14, 27 -- Received: by 10.114.76.16 with HTTP; Tue, 23 Oct 2007 06:35:32 -0700 (PDT) 14, 27 -- Message-ID: {1a0a54550710230635m68662297w460f5e2d157f0522-at-mail.gmail.com} 14, 27 -- Date: Tue, 23 Oct 2007 09:35:32 -0400 14, 27 -- From: "D.N. Leonard" {donovan.leonard-at-gmail.com} 14, 27 -- To: Microscopy-at-microscopy.com 14, 27 -- Subject: Re: Advice needed for using Hitachi TM-1000 desktop SEM 14, 27 -- MIME-Version: 1.0 14, 27 -- Content-Type: text/plain; charset=ISO-8859-1 14, 27 -- Content-Transfer-Encoding: 7bit 14, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I have seen precipitation of something (???) from Epo-Fix after several years shelf-life. Threw it away and purchased new batch. If you care at all about your sample, never compromise quality with any suspicious preparation materials.
I am sure that if Chuck Garber were around, he would tell us all exactly what the precipitating component is.
Regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations." Tom Magliozzi
nizets2-at-yahoo. com To gary.m.brown-at-exxonmobil.com 10/23/07 02:53 cc AM Subject [Microscopy] dirty Epon Please respond to nizets2-at-yahoo. com
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi all!
I would like to wake up the list (is it hibernating?) with the following issue. I prepare Epon for 15 years and it was always clear, like a good old Johnnie Walker (and almost as toxic :-D). Today, after I added BDMA and I carefully mixed as usual, I found it full of small dirt, like precipitates. At the beginning I thought I mixed too strongly (sometimes I cannot control my strength ;-)) and these were small bubbles so I let it calm down, but to no avail. These are definitely NOT small bubbles. It never happened before and I wonder what I should do.
- Do somebody have had this problem before? - Can I use this mixture? - What went wrong? There is no expiry date on none of the products but they are not older than y few years (stored at RT). I am afraid that, if I prepare it again with the same products, I will get the same result.
Best regards, Stephane
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==============================Original Headers============================== 6, 21 -- From nizets2-at-yahoo.com Tue Oct 23 02:51:02 2007 6, 21 -- Received: from web37411.mail.mud.yahoo.com (web37411.mail.mud.yahoo.com [209.191.91.143]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l9N7p2Nc002784 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 23 Oct 2007 02:51:02 -0500 6, 21 -- Received: (qmail 50406 invoked by uid 60001); 23 Oct 2007 07:51:02 -0000 6, 21 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 21 -- s=s1024; d=yahoo.com; 6, 21 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID;
AFM Technical Sales Representatives-US and UK Asylum Research has openings available for Technical Sales Representatives in the United States and in our Oxford, UK office. The candidates should have knowledge of AFM, instrumentation, and application experience in the areas of physics, electrical measurements, material science, polymers, and/or bioscience, or similar scientific instrumentation experience. The individuals will be responsible for all sales activities including generating new leads and lead follow-up, attending trade shows, customer quotations, technical presentations, product demonstrations, as well as customer application support. There will be frequent interface with sales/ marketing and application scientists. Must have excellent communications skills, both written and oral. There will be frequent travel.
To apply for a position, please send your resume in a PDF file to jobs-at-AsylumResearch.com and indicate the job position in the subject line. You may also fax it to 805-696-6444. Please also include a cover letter, resume and salary requirements. Additional information can be found at http://www.asylumresearch.com/About/About.shtml#CR
Terry Mehr
Asylum Research
==============================Original Headers============================== 4, 13 -- From terry-at-AsylumResearch.com Tue Oct 23 15:46:45 2007 4, 13 -- Received: from exchange.AsylumResearch.com (exchange.asylumresearch.com [207.154.79.129]) 4, 13 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9NKkiaE009178 4, 13 -- for {Microscopy-at-microscopy.com} ; Tue, 23 Oct 2007 15:46:45 -0500 4, 13 -- Mime-Version: 1.0 (Apple Message framework v752.2) 4, 13 -- Content-Transfer-Encoding: 7bit 4, 13 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 4, 13 -- To: Microscopy-at-microscopy.com 4, 13 -- From: Terry Mehr {terry-at-AsylumResearch.com} 4, 13 -- Subject: Job Openings- AFM Technical Representatives-Asylum Research 4, 13 -- Date: Tue, 23 Oct 2007 13:46:37 -0700 4, 13 -- X-Mailer: Apple Mail (2.752.2) 4, 13 -- Message-ID: {1IkQeB-0006j6-Op-at-exchange.AsylumResearch.com} ==============================End of - Headers==============================
I recently saw this instrument at the M&M conference in Ft. Lauderdale, FL. I was impressed by it, much more so than I was by the FEI Phenom. In particular, since I am a high school teacher, I challenged the sales person. Since I deal with students that will go out and find whatever they want (And you'd be surprised what they find! One student handed me a booger on a slide once and wanted to look at it under a light microscope!)
I happened to be eating my lunch at the time of my demo, which consisted of a burger and fries. Expecting a sample-prep nightmare, I offered a french fry from my plate, which the sales rep quickly broke off, placed on a stub, inserted it into the microscope, and viewed it. It wasn't one of those dry, crusty ones, either. It was a hot, oily, wet fry.
I was amazed at the imaging capabilities on such a bad specimen.
My take on it, though, is that Hitachi Canada has the better accessories for it. They have a motorized stage control as well as a nicer EDS unit that attaches to it. They also have a switchbox which overcomes my one complaint about the instrument- the lack of ability to switch from topo to compo mode on the BSE detector.
All in all, my impression (Given what I saw, as well as the stories I heard from using it in middle- and high-schools for demos) was that it is a very versatile instrument that would be hard to break.
Don't take this as a warranty, though! Inspect your boogers at your own risk!
--Justin A. Kraft Leadership Academy West West Palm Beach, FL
==============================Original Headers============================== 7, 31 -- From kraftpiano-at-gmail.com Tue Oct 23 21:35:37 2007 7, 31 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.189]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9O2Zb7A029312 7, 31 -- for {microscopy-at-microscopy.com} ; Tue, 23 Oct 2007 21:35:37 -0500 7, 31 -- Received: by rv-out-0910.google.com with SMTP id k20so44698rvb 7, 31 -- for {microscopy-at-microscopy.com} ; Tue, 23 Oct 2007 19:35:36 -0700 (PDT) 7, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 31 -- d=gmail.com; s=beta; 7, 31 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 7, 31 -- bh=+berUsfq7kkAEpDTyQOGezQ6G8Qj7BmKPtSRTBgbOOQ=; 7, 31 -- b=OO/0o6ZdPnGaEeEMVb1eB72mGxvDaY/w3q2MFKN9d0r6ZaDvcdgK8Zi1S/UKuizTjhdmpBDtopXYTSaFiHqj+NZS4y+I/N3XC0bgQIYmPmOF7s1NnWYlTf02lJJF6C/8xl+30yaVcqboYoae3YI88aBLT432inASdbDO2OUg6g4= 7, 31 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 31 -- d=gmail.com; s=beta; 7, 31 -- h=received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 7, 31 -- b=JlZd3qgM/uYVWtzVoupygEnAfjK9Ukfgisk8MqjLQP+R3o4z0AzmHYXzqL0JJlcpLgpaxY4qssUUBgu2qyvu+Kixkt+o7trxEVQSrJNB8GaZaST3FVPS1L6ZqBsVhoSbObbUktj4JHOOKnSik/ueXNCa1lWtdgkMCVzzDfwSjZU= 7, 31 -- Received: by 10.141.90.17 with SMTP id s17mr47058rvl.1193193336438; 7, 31 -- Tue, 23 Oct 2007 19:35:36 -0700 (PDT) 7, 31 -- Received: by 10.141.206.17 with HTTP; Tue, 23 Oct 2007 19:35:36 -0700 (PDT) 7, 31 -- Message-ID: {25e2b0d20710231935k620d5500s5d2cd5538256a13e-at-mail.gmail.com} 7, 31 -- Date: Tue, 23 Oct 2007 22:35:36 -0400 7, 31 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 7, 31 -- To: pjm-at-gol.com, microscopy-at-microscopy.com 7, 31 -- Subject: Re: [Microscopy] Advice needed for using Hitachi TM-1000 desktop SEM 7, 31 -- In-Reply-To: {25e2b0d20710231448w3dc5a4b9ia3d2c8996c83dec2-at-mail.gmail.com} 7, 31 -- MIME-Version: 1.0 7, 31 -- Content-Type: text/plain; charset=ISO-8859-1 7, 31 -- Content-Transfer-Encoding: 7bit 7, 31 -- Content-Disposition: inline 7, 31 -- References: {200710231138.l9NBcJ7S031769-at-ns.microscopy.com} 7, 31 -- {25e2b0d20710231447q4c5bea68s978ef1f9d0a0592c-at-mail.gmail.com} 7, 31 -- {25e2b0d20710231448w3dc5a4b9ia3d2c8996c83dec2-at-mail.gmail.com} ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ncoombs-at-chem.utoronto.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ncoombs-at-chem.utoronto.ca Name: Neil Coombs
Organization: Centre for Nanostructure Imaging, University of Toronto
Question: One of our graduate students is working on a block copolymer system (NIPAM/PDMA). The polymers are relatively similar in structure, however they differ in that one contains a secondary amine and the other a tertiary amine. We are looking for a heavy metal stain (and/or staining conditions/protocols) that is likely to preferentially attach to one of these groups. Any help would be appreciated!
Dr. N. Coombs Centre for Nanostructure Imaging Dept. of Chemistry, University of Toronto
Neil Coombs asked about selective staining of block copolymers, in order to image their nanostructure. I don't have an answer to the chemical question but instead suggest trying AFM phase imaging instead of TEM. Atomic Force Microscopy has been very successful in getting image contrast between different polymer domains without staining. The mechanical interaction of the AFM probe with the sample surface senses local differences in stiffness and/or adhesion in order to create contrast. The AFM can scan as-produced exterior surfaces of test samples and on ultramicrotomed blocks in order to see inside the bulk. There is no need to create a good thin section, just a smooth block face. Examples of phase images can be found at: www.asmicro.com/Applications/phase.htm
Disclaimers: My company provides AFM analysis services, training and consulting, and we buy and sell used AFMs that often include phase capability.
Historical background: I was the first person outside Digital Instruments to make a AFM phase image while tapping on the surface (11/1994). For about a year after that, I was the only person in the world promoting phase imaging as a technique for material analysis. I still get a kick from telling people to try it.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] =============================================
----- Original Message ----- From: ncoombs-at-chem.utoronto.ca To: donc-at-asmicro.com Sent: Wednesday, October 24, 2007 11:23 PM Subject: [a] [Microscopy] viaWWW: Differential Polymer Staining
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ncoombs-at-chem.utoronto.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ncoombs-at-chem.utoronto.ca Name: Neil Coombs
Organization: Centre for Nanostructure Imaging, University of Toronto
Question: One of our graduate students is working on a block copolymer system (NIPAM/PDMA). The polymers are relatively similar in structure, however they differ in that one contains a secondary amine and the other a tertiary amine. We are looking for a heavy metal stain (and/or staining conditions/protocols) that is likely to preferentially attach to one of these groups. Any help would be appreciated!
Dr. N. Coombs Centre for Nanostructure Imaging Dept. of Chemistry, University of Toronto
A position is available immediately for a trained individual to join the Electron Microscopy Centre at Imperial College, South Kensington Campus, LONDON (UK).
The newly-created Centre has state-of-the-art infrastructure and a wide range of techniques for visualisation of biological structures from macromolecules to cellular organisation. You will have a biological or technical degree, experience in cell biology research environment, as well as in specimen preparation techniques for Transmission Electron Microscopy and the ability to perform ultra-thin sectioning. You will be able to communicate on specialised technical issues, have good analytical and problem solving skills and be computer literate. In addition you will have excellent communication skills, the ability to be precise and meticulous with strong attention to detail and the ability to exercise your initiative to work independently and as part of a team. You will be expected to perform some routine EM for Imperial research groups and provide training to new users.
The position offers opportunities for participating in research projects, methods development and learning techniques such as Electron Tomography and high resolution EM.
A job description and person specification can be obtained from the following link:
https://www.imperial.ac.uk/employment
Further details can be obtained from Dr Raffaella Carzaniga on r.carzaniga-at-imperial.ac.uk
Closing date: 19 November 2007.
==============================Original Headers============================== 13, 29 -- From r.carzaniga-at-imperial.ac.uk Thu Oct 25 11:18:10 2007 13, 29 -- Received: from smtp1.cc.ic.ac.uk (smtp1.cc.ic.ac.uk [155.198.5.155]) 13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9PGIAcO023381 13, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Oct 2007 11:18:10 -0500 13, 29 -- Received: from icexp1.cc.ic.ac.uk ([155.198.3.41] helo=icexp1.ic.ac.uk) 13, 29 -- by smtp1.cc.ic.ac.uk with smtp (Exim 4.67) 13, 29 -- (envelope-from {r.carzaniga-at-imperial.ac.uk} ) 13, 29 -- id 1Il5PN-00064P-RM 13, 29 -- for Microscopy-at-microscopy.com; Thu, 25 Oct 2007 17:18:09 +0100 13, 29 -- Received: from icex6.ic.ac.uk ([155.198.3.6]) by icexp1.ic.ac.uk with Microsoft SMTPSVC(6.0.3790.3959); 13, 29 -- Thu, 25 Oct 2007 17:17:38 +0100 13, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 13, 29 -- Content-class: urn:content-classes:message 13, 29 -- MIME-Version: 1.0 13, 29 -- Content-Type: text/plain; 13, 29 -- charset="iso-8859-1" 13, 29 -- Disposition-Notification-To: "Carzaniga, Raffa" {r.carzaniga-at-imperial.ac.uk} 13, 29 -- Subject: TEM/position available-Electron Microscopy Technician-London-UK 13, 29 -- Date: Thu, 25 Oct 2007 17:17:37 +0100 13, 29 -- Message-ID: {B32CD30A6C6DB6419B2FB76DB496740AE12C29-at-icex6.ic.ac.uk} 13, 29 -- X-MS-Has-Attach: 13, 29 -- X-MS-TNEF-Correlator: 13, 29 -- Thread-Topic: TEM/position available-Electron Microscopy Technician-London-UK 13, 29 -- Thread-Index: AcgXIo8F9dlgCj6QS5SzAlfqUZzJAQ== 13, 29 -- From: "Carzaniga, Raffa" {r.carzaniga-at-imperial.ac.uk} 13, 29 -- To: {Microscopy-at-microscopy.com} 13, 29 -- X-OriginalArrivalTime: 25 Oct 2007 16:17:38.0276 (UTC) FILETIME=[8F6E5A40:01C81722] 13, 29 -- Content-Transfer-Encoding: 8bit 13, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l9PGIAcO023381 ==============================End of - Headers==============================
} Date: Thu, 25 Oct 2007 13:48:51 -0500 } To: Zaluzec-at-MSA.MICROSCOPY.COM } From: sun1grass-at-yahoo.com () } Subject: [Filtered] AskAMicroscopist: softwares for crystal structure anlysis } } Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sun1grass-at-yahoo.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, October 25, 2007 at 13:48:51 } --------------------------------------------------------------------------- } } Email: sun1grass-at-yahoo.com } Name: Fengli Wang } } Organization: Colorado School of Mines } } Education: Graduate College } } Location: golden, CO } } Question: Hi, } } Can anybody recommend some good softwares for crystal structure anlysis? Where can I get pcpwin? Thank you very much. } } Sincerely, } Fengli } } } --------------------------------------------------------------------------- }
==============================Original Headers============================== 1, 11 -- From zaluzec-at-ultra5.microscopy.com Thu Oct 25 21:23:39 2007 1, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 1, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9Q2NaNf018751 1, 11 -- for {microscopy-at-microscopy.com} ; Thu, 25 Oct 2007 21:23:38 -0500 1, 11 -- Mime-Version: 1.0 1, 11 -- Message-Id: {p06240800c346fffae51f-at-[206.69.208.22]} 1, 11 -- Date: Thu, 25 Oct 2007 21:23:34 -0500 1, 11 -- To: microscopy-at-microscopy.com 1, 11 -- From: Ask-A-Microscopist {zaluzec-at-ultra5.microscopy.com} 1, 11 -- Subject: AskAMicroscopist : softwares for crystal structure anlysis 1, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wlehman-at-bu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wlehman-at-bu.edu Name: Tori Hatch
Organization: Boston University
Title-Subject: [Filtered] position available
Question: POSITION AVAILABLE Senior Electron Microscopy Technician
A Senior Electron Microscopy Technician is sought to perform duties in support of research work on the structure of actin filament complexes that (1) are associated with cardiac and skeletal muscle regulatory proteins to control muscle activity and (2) interact with smooth muscle actin-binding proteins to modulate the assembly of the smooth muscle cytoskeleton. Applicants must have experience in high-resolution EM work and first-rate facility with computer-assisted image analysis. The candidate should have a Bachelorís degree and several years of experience. Prior familiarity with preserving and recording macromolecular assemblies in negative stain and by cryo-EM methods would be invaluable. Experience in supervising graduate students and post-doctoral fellows would also be important.
Interested applicants should send their CV with three references to:
Dr. William Lehman Professor of Physiology & Biophysics Department of Physiology & Biophysics Boston University School of Medicine 715 Albany Street Boston, MA 02118
Or email:
wlehman-at-bu.edu
Dr. Lehmanís research program is summarized on his home page:
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Email: DLowry-at-asu.edu Name: David Lowry
Organization: Arizona State University
Title-Subject: [Filtered] part for Reichert Ultracut-E microtome
Question: we have a Reichert Ultracut-E microtome that needs a part, specifically the entire block-holder apparatus that clamps into the cantilever arm, with 360ƒ rotating chuck and arc segment for block adjustment. If anyone may have an old, infirm Reichert ultramicrotome in the corner from which they would be willing to sell or donate this particular part, please contact me off-line. Thank you,
To accommodate new light microscopes coming to our facility we need to split a couple of rooms with black-out curtains. We've seen some nice set-ups with curtains that slide on metal rails installed in the ceiling, can people recommend vendors for something like that?
Thanks in advance.
-- G. Esteban Fernandez, Ph.D. Associate Director Molecular Cytology Research Core Facility University of Missouri 1201 E. Rollins St. Columbia, MO 65211
(573)882-4895
http://www.biotech.missouri.edu/mcc
==============================Original Headers============================== 4, 23 -- From fernandezg-at-missouri.edu Mon Oct 29 09:09:22 2007 4, 23 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9TE9M21008261 4, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 29 Oct 2007 09:09:22 -0500 4, 23 -- Received: from UM-XMAIL03.um.umsystem.edu ([209.106.228.3]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 4, 23 -- Mon, 29 Oct 2007 09:09:21 -0500 4, 23 -- x-mimeole: Produced By Microsoft Exchange V6.5 4, 23 -- Content-class: urn:content-classes:message 4, 23 -- MIME-Version: 1.0 4, 23 -- Content-Type: text/plain; 4, 23 -- charset="iso-8859-1" 4, 23 -- Subject: LM vendors for black-out curtains 4, 23 -- Date: Mon, 29 Oct 2007 09:09:21 -0500 4, 23 -- Message-ID: {002C76CE90154E4EAD095153F371D71D043EF495-at-UM-XMAIL03.um.umsystem.edu} 4, 23 -- X-MS-Has-Attach: 4, 23 -- X-MS-TNEF-Correlator: 4, 23 -- Thread-Topic: LM vendors for black-out curtains 4, 23 -- Thread-Index: AcgaNU1QgMxd+XWBSMipvxebgyJIfQ== 4, 23 -- From: "Fernandez, Gerardo E." {fernandezg-at-missouri.edu} 4, 23 -- To: {Microscopy-at-microscopy.com} 4, 23 -- X-OriginalArrivalTime: 29 Oct 2007 14:09:21.0733 (UTC) FILETIME=[4D961750:01C81A35] 4, 23 -- Content-Transfer-Encoding: 8bit 4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l9TE9M21008261 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (xmoeinx-at-yahoo.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, October 26, 2007 at 06:14:07 ---------------------------------------------------------------------------
Email: xmoeinx-at-yahoo.com Name: Moein Arzi
Organization: sharif
Education: Graduate College
Location: Tehran, Iran
Question: what is a solution of silver jet polish?
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bowlingads-at-hotmail.co.uk) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, October 28, 2007 at 10:31:23 ---------------------------------------------------------------------------
Email: bowlingads-at-hotmail.co.uk Name: Adam husband
Organization: shef biomed
Education: Undergraduate College
Location: sheffield, yorkshire, UK
Question: why is resolution in the z-axis is different from that in the x and y axes in confocal microscopy? this question is really bugging me, cant find many articles that explain the differences between the axes.
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Email: yyang-at-cae.wisc.edu Name: Yong Yang
Organization: University of WIsconsin-Madison
Title-Subject: [Filtered] Philips CM 200 specimen holder
Question: Hell everyone,
I am looking for a TEM specimen holder for Philips CM 200, and it should be double tilt (not very large angle, just regular one), but one important feature is that the sample (eletropolished, 3mm disk) can be fastened tightly on the holder to avoid be sucked onto the poles since my samples are ferritic. And our CM200 is special, since it has the narrow pole-piece gap UltraTwin pole piece.
Adam, I've never used a confocal, but it seems to me that the answer is the same as for other types of microscopes: the mechanism is completely different for Z than for X & Y. X & Y resolution is much discussed and is based upon wavelength contraints, numerical aperture contraints, working distance contraints and similar items with electrons (instead of light). Since the depth of field is going to be the biggest factor in determining the Z resolution (which is usually considerably greater (worse) than the XY resolution) I suspect that your Z resolution is going to be determined by the Z axis of the stage (or however the confocal determines its slices) in conjunction with the depth of field at any scanned point. I suspect that, like an SEM, the scanning point by point greatly increases the depth of field and therefore hurts Z resolution even more.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: bowlingads-at-hotmail.co.uk [mailto:bowlingads-at-hotmail.co.uk] Sent: Monday, October 29, 2007 10:36 AM To: kenconverse-at-qualityimages.biz
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (bowlingads-at-hotmail.co.uk) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, October 28, 2007 at 10:31:23 ---------------------------------------------------------------------------
Email: bowlingads-at-hotmail.co.uk Name: Adam husband
Organization: shef biomed
Education: Undergraduate College
Location: sheffield, yorkshire, UK
Question: why is resolution in the z-axis is different from that in the x and y axes in confocal microscopy? this question is really bugging me, cant find many articles that explain the differences between the axes.
Here is the November 2007 Microscopy Today table of contents. I will close the subscription list for this issue on Thursday November 1st, 2007.
Microscopists in North America and MSA members anywhere qualify for free subscriptions. Anyone else may subscribe for US$50 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com . Subscription rate for non-qualified readers will go to US$60 in 2008.
Thank you.
Ron Anderson, Editor ======================== Establishing the Initial Embryonic Axis Stephen W. Carmichael and Gary C. Schoenwolf1, Mayo Clinic and the 1University of Utah
Beyond Death: Forensic Investigations of pre-Columbian Mummies from the Tarapacá Valley, Chile, Using Variable Pressure SEM and Raman Spectroscopy S.V. Prikhodko, C. Fischer, R. Boytner, M. C. Lozada, M. Uribe, I. Kakoulli, UCLA, U. of Chicago, and U. de Chile
Applications of the Helium Ion Microscope Larry Scipioni, Lewis Stern, and John Notte, ALIS Business Unit, Carl Zeiss SMT, Inc., Peabody, MA
Methods of Maximizing X-ray Detection in SEMs David Rohde, Patrick Camus, Thermo Fisher Scientific, Madison, WI
Three Dimensional Imaging of Structure and Flow?Critical to Advances in Microfluidics Carlos Hidrovo1, and Terence Lundy2, 1Stanford University 2Hyphenated Systems, Burlingame, CA
Is a Cs Corrector Necessary for Lorentz Vector Field Tomography? Charudatta Phatak, Marc De Graef, Carnegie Mellon University, Pittsburgh, PA
TEM of Bacteriophages Found in Marine Sources A. Fejeran1, J. Polanco2, G. Lander3, T. Ajero Jr3, B. Carragher3, C. S. Potter,3 1Mount Miguel High School, Spring Valley, CA, 2Hilltop High School, Chula Vista, CA, 3Scripps Research Institute, La Jolla, CA
Nanoscale Thermal Property Characterization of Automotive Polymer Coatings Louis T. Germinario, Eastman Chemical Company, Kingsport, TN
Computer-Controlled Polishing System For Preparing Multiple Pre-FIB TEM Specimens D. J. MacMahon1 and E. Raz-Moyal2, Micron Technology, Inc. and 2Gatan, Inc., Manassas, VA and Pleasanton, CA
Tungsten Filament Heating Effects Paul Beauregard, Microscopist, Greensburg, TN
The Database Solution to Particle-by-Particle Analysis of Mixed Mineral Dusts B.R. Strohmeier, K.L. Bunker, K.E. Harris, R. Hoch, and R.J. Lee, RJ Lee Group, Inc., Monroeville, PA
Table Top SEM Utilization in a High School Nanotechnology Course Leonard, D.N., Appalachian State University, Boone, NC
Microscopy for Children Caroline Schooley, Project MICRO Coordinator, MSA
Industry News
NetNotes SAMPLE PREPARATION ? staining lipids for TEM SAMPLE PREPARATION - staining polysaccharides for TEM SAMPLE PREPARATION ? Thermanox coverslip SAMPLE PREPARATION - dewaxing leaf surface for SEM SAMPLE PREPARATION - osmium tetroxide on Lowicryl sections SAMPLE PREPARATION ? premature polymerization SAMPLE PREPARATION - Au on C and Al/W dendrites SAMPLE PREPARATION ? sputter coater glass chamber SAMPLE PREPARATION - sputter coater thickness EM - Wi-Fi and RF interference TEM ? contamination SEM - tilt correction SEM - wt% or atom%? SEM - secondary electron detection EDX - SDD detectors ELECTRON DIFFRACTION XRF in SEM-ED GENERAL TECHNIQUES - canned air
Advertiser's Index
==============================Original Headers============================== 21, 18 -- From microscopytoday-at-tampabay.rr.com Tue Oct 30 08:54:33 2007 21, 18 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.122]) 21, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9UDsX4E029829 21, 18 -- for |--Microscopy-at-Microscopy.Com--|; Tue, 30 Oct 2007 08:54:33 -0500 21, 18 -- Received: from [127.0.0.1] (really [24.73.73.214]) 21, 18 -- by hrndva-omta03.mail.rr.com with ESMTP 21, 18 -- id |--20071030135430.VMEK4063.hrndva-omta03.mail.rr.com-at-[127.0.0.1]--|; 21, 18 -- Tue, 30 Oct 2007 13:54:30 +0000 21, 18 -- Message-ID: |--47273793.8060407-at-tampabay.rr.com--| 21, 18 -- Date: Tue, 30 Oct 2007 09:54:27 -0400 21, 18 -- From: Microscopy Today |--microscopytoday-at-tampabay.rr.com--| 21, 18 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 21, 18 -- MIME-Version: 1.0 21, 18 -- To: Listserver |--Microscopy-at-Microscopy.Com--|, 21, 18 -- Confocal Listserver |--confocal-at-listserv.buffalo.edu--| 21, 18 -- Subject: Microscopy Today November 2007 Table of Contents 21, 18 -- Content-Type: text/plain; charset=windows-1252; format=flowed 21, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
==============================Original Headers============================== 25, 17 -- From randerson20-at-tampabay.rr.com Tue Oct 30 08:56:52 2007 25, 17 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.123]) 25, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9UDuqfQ029893 25, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 30 Oct 2007 08:56:52 -0500 25, 17 -- Received: from [127.0.0.1] (really [24.73.73.214]) 25, 17 -- by hrndva-omta05.mail.rr.com with ESMTP 25, 17 -- id {20071030135651.ORUU4100.hrndva-omta05.mail.rr.com-at-[127.0.0.1]} 25, 17 -- for {Microscopy-at-Microscopy.Com} ; Tue, 30 Oct 2007 13:56:51 +0000 25, 17 -- Message-ID: {47273820.9010403-at-tampabay.rr.com} 25, 17 -- Date: Tue, 30 Oct 2007 09:56:48 -0400 25, 17 -- From: Ronald Anderson {randerson20-at-tampabay.rr.com} 25, 17 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 25, 17 -- MIME-Version: 1.0 25, 17 -- To: Listserver {Microscopy-at-Microscopy.Com} 25, 17 -- Subject: Microscopy Today November 2007 Table of Contents] 25, 17 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 25, 17 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: eoptics-at-mcmaster.ca Name: Fred Pearson
Organization: McMaster University
Title-Subject: [Filtered] TEM--CM12 Quad Serial Interface
Question: Good Day:
I am looking for a functioning "quad serial interface board" for the Philips CM series TEM. Ours is a CM12. If you have such item that is not being used and for sale please reply.
I'd like to mine the collective knowledge of this community once again. Every now and then, someone asks me to look at a solution of just surfactant or bile salt micelles (i.e., sodium taurocholate/lecithin or Polysorbate 80). They give me literature citing scattering studies that show these micelles should be of some "TEM appropriate" size. They usually request cryo TEM as the sample prep method, since they don't want to perturb the system by introducing contrast agents.
What inevitably happens is that I prepare the solution for cryo TEM (vitrify solution on Lacey Carbon grid and image) and see nothing. So here are my questions:
1. Does anyone work with micelle systems such as these, or is it a lost cause due to inherent low contrast, or reasons I'm not aware of? I've done some preliminary literature searches, and found nothing specifically on TEM imaging of surfactants or bile salt micelles alone in solution (which may have already answered my question).
2. If so, what type of sample prep or contrast agents do you use?
Any information is much appreciated. Thanks, Jessica
____________________ Jessica Cervantes, MS Research Chemist
Bend Research Inc 64550 Research Rd Bend, OR 97701
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==============================Original Headers============================== 14, 14 -- From cervantes-at-bendres.com Tue Oct 30 12:13:40 2007 14, 14 -- Received: from BRIEX04A.bri.local (bc161112.bendcable.com [216.228.161.112]) 14, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9UHDdbU028964 14, 14 -- for {Microscopy-at-microscopy.com} ; Tue, 30 Oct 2007 12:13:40 -0500 14, 14 -- MIME-Version: 1.0 14, 14 -- Content-Type: text/plain; 14, 14 -- charset="us-ascii" 14, 14 -- Subject: TEM Help with Imaging of Micelle Solutions 14, 14 -- Date: Tue, 30 Oct 2007 10:13:39 -0700 14, 14 -- Message-ID: {8943D65F9AD70E4488AD6DE09F15088768C81D-at-BRIEX04A} 14, 14 -- From: "Cervantes, Jessica" {cervantes-at-bendres.com} 14, 14 -- To: {Microscopy-at-microscopy.com} 14, 14 -- Content-Transfer-Encoding: 8bit 14, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id l9UHDdbU028964 ==============================End of - Headers==============================
I have a question (actually, a set of questions) about Quantimix capsules. I know they are usable for growing sell cultures. But is it possible to observe these sells while they are still alive, without fixation and staining? Do I have to use special "Imaging Buffer" or growth media will be OK? What level of magnifications can I expect?
Thanks,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
Dear Jessica, I think that your vitreous ice cryoTEM approach is the best choice to image micelles. Several years ago we were studying gallstone formation and attempted to visualize by cryoTEM the earliest stages of cholesterol nucleation using a supersaturated model bile composed of cholesterol, lecithin, and taurocholate in an aqueous solution with 0.15M NaCl. We were able to see various types of unilamellar and multilamellar vesicles in a background of micelles. Our homemade holey carbon films were relatively thick because we were trying to capture particles expected to be much larger than micelles. You might want to consider very thin carbon layers. I think we were single side blotting to concentrate the particles; there may be some trial and error in working out the blotting times You can take a look at the micrographs in the paper in Biophysical Journal Vol. 76, pp.1436-1451. There are more technical details and possibly some useful references. Good luck, Don
Donald Gantz Research Histologist/Electron Microscopist Dept. Physiology and Biophysics Center for Advanced Biomedical Research Boston University School of Medicine 715 Albany Street Boston, MA 02118 email: gantz-at-bu.edu
==============================Original Headers============================== 3, 24 -- From gantz-at-bu.edu Tue Oct 30 16:18:37 2007 3, 24 -- Received: from relay17.bu.edu (relay17.bu.edu [128.197.27.68]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9ULIaaa029158 3, 24 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 30 Oct 2007 16:18:36 -0500 3, 24 -- X-Envelope-From: gantz-at-bu.edu 3, 24 -- Received: from biophysics1.bumc.bu.edu (biophysics1.bumc.bu.edu [155.41.208.134]) 3, 24 -- by relay17.bu.edu (8.13.1/8.13.1) with ESMTP id l9ULIHCj022133 3, 24 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 30 Oct 2007 17:18:17 -0400 3, 24 -- Received: from gantz (cabr3-dhcp-208-153.bumc.bu.edu [155.41.208.153]) 3, 24 -- by biophysics1.bumc.bu.edu (8.13.8/8.13.8) with SMTP id l9ULIHS7018339 3, 24 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 30 Oct 2007 17:18:17 -0400 3, 24 -- Message-ID: {003801c81b3a$5b271440$99d0299b-at-gantz} 3, 24 -- From: "Don Gantz" {gantz-at-bu.edu} 3, 24 -- To: {Microscopy-at-MSA.Microscopy.com} 3, 24 -- Subject: TEM Help with Imaging of Micelle Solutions 3, 24 -- Date: Tue, 30 Oct 2007 16:18:02 -0500 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Type: text/plain; 3, 24 -- charset="iso-8859-1" 3, 24 -- Content-Transfer-Encoding: 7bit 3, 24 -- X-Priority: 3 3, 24 -- X-MSMail-Priority: Normal 3, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1807 3, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1807 ==============================End of - Headers==============================
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Email: Angel.Paredes-at-uth.tmc.edu Name: Angel
Organization: University of Texas Health Science Center
Title-Subject: [Filtered] Problem with: Tecnai Polara G2 New Film camera system
Question: Hi,
We have a 300 kV Tecnai Polara G2. We now have the new redesigned low noise camera boxes with the new film cassettes. For those who have gotten the new camera system, I am have a lot of camera jams. Four since last Friday to this past Monday. Is anyone else having problems with jams using this new film camera system? Has anyone had any success fixing the problem?
Angel: Yes, we have been having problems as well. However, we have not used enough film to get a definite handle on the problem (or problems?).
1. There is a pin on a metal plate that is inside the viewing chamber on the end nearest the user. If this pin does not come in contact with the slide from the film box the films will jam going back into the camera. You can see if that is the problem because each film plate should be absolutely horizontal. With the bent pin it won't drop the film plate evenly. That plate with the pin attached bends easily. It can be bent back again but FEI has them and if you wait long enough you might be able to get one.
2. Make sure the slide mechanism underneath is tight. It has four screws, if I remember right, and if there is too much play you will have problems.
There is a difference in the two problems. With #1 you actually get a jammed plate with #2 the plate doesn't slide all the way into the beam and you get a film that is exposed on only half of it.
Norm Olson UCSD
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==============================Original Headers============================== 11, 25 -- From nholson-at-ucsd.edu Tue Oct 30 17:36:02 2007 11, 25 -- Received: from outbound2.ucsd.edu (outbound2.ucsd.edu [132.239.1.206]) 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9UMa2JU022573 11, 25 -- for {microscopy-at-microscopy.com} ; Tue, 30 Oct 2007 17:36:02 -0500 11, 25 -- Received: from smtp.ucsd.edu (smtp.ucsd.edu [132.239.1.49]) 11, 25 -- by outbound2.ucsd.edu (8.13.6/8.13.6) with ESMTP id l9UMZs73009088 11, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK); 11, 25 -- Tue, 30 Oct 2007 15:35:55 -0700 (PDT) 11, 25 -- DomainKey-Signature: a=rsa-sha1; s=2007001; d=ucsd.edu; c=simple; q=dns; 11, 25 -- b=iWdEAyvKP0L/yTZa91LRml1kRi81s5z963ILk6kdg2BMV1rzmXhV+RLSAT4NNwYAb 11, 25 -- l8flPsS4QV1OQraqAONXQ== 11, 25 -- Received: from [132.239.70.186] (patrick-70-186.ucsd.edu [132.239.70.186]) 11, 25 -- by smtp.ucsd.edu (8.13.6/8.13.6) with ESMTP id l9UMZrB1084649; 11, 25 -- Tue, 30 Oct 2007 15:35:54 -0700 (PDT) 11, 25 -- X-Authentication-Warning: smtp.ucsd.edu: Host patrick-70-186.ucsd.edu [132.239.70.186] claimed to be [132.239.70.186] 11, 25 -- Mime-Version: 1.0 11, 25 -- Message-Id: {p0624080cc34d5ffaef09-at-[132.239.70.186]} 11, 25 -- In-Reply-To: {200710302221.l9UMLt5l012426-at-ns.microscopy.com} 11, 25 -- References: {200710302221.l9UMLt5l012426-at-ns.microscopy.com} 11, 25 -- Date: Tue, 30 Oct 2007 15:36:23 -0700 11, 25 -- To: Angel.Paredes-at-uth.tmc.edu, microscopy-at-microscopy.com 11, 25 -- From: Norm Olson {nholson-at-ucsd.edu} 11, 25 -- Subject: Re: [Microscopy] viaWWW: Problem with: Tecnai Polara G2 New Film 11, 25 -- camera system 11, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Contact Brigitte Sternberg - Brigitte P Sternberg {brigittnanoanalytical-at-yahoo.com} . She has done a great deal of cryo work in this area. I had a chance to learn more about it at the recent SPIE meeting in San Diego and included that info in an upcoming article in America Lab (November, most likely): "Focus on Microscopy" column which can be found under "ARTICLES" at www.iscpubs.com. Briggitte's website is http://nanoanalytical.netfirms.com
Hope this is helpful.
Barbara Foster, President
We've moved! Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme E: bfoster-at-mme1.com W: www.MicroscopyEducation.com
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P. S. Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Just call us here in the MME office for details.
At 03:33 PM 10/30/2007, gantz-at-bu.edu wrote:
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==============================Original Headers============================== 20, 19 -- From bfoster-at-mme1.com Tue Oct 30 17:14:08 2007 20, 19 -- Received: from smtp102.sbc.mail.mud.yahoo.com (smtp102.sbc.mail.mud.yahoo.com [68.142.198.201]) 20, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l9UME83l010072 20, 19 -- for |--microscopy-at-microscopy.com--|; Tue, 30 Oct 2007 17:14:08 -0500 20, 19 -- Message-Id: |--200710302214.l9UME83l010072-at-ns.microscopy.com--| 20, 19 -- Received: (qmail 71515 invoked from network); 30 Oct 2007 22:14:08 -0000 20, 19 -- Received: from unknown (HELO barbsd505.mme1.com) (bfostermme-at-sbcglobal.net-at-68.94.39.83 with login) 20, 19 -- by smtp102.sbc.mail.mud.yahoo.com with SMTP; 30 Oct 2007 22:14:07 -0000 20, 19 -- X-YMail-OSG: zCf1CHwVM1mjsnresXNNjy4g4mQgfQLTWsX4qahsVmP9T8UgjQUeW6cEmCamU0KhjUXzbSG2GQwZcBmAqa9kxQTVJ7Ot9hIJtFYba__K1.CTbglXJNSfbnv1G5OCVcb_7g2A6wegFnKVDlkmeQUizrIsQnBlqUg6eqBuEoNtbF0A 20, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 20, 19 -- Date: Tue, 30 Oct 2007 17:11:29 -0600 20, 19 -- To: gantz-at-bu.edu, microscopy-at-microscopy.com 20, 19 -- From: Barbara Foster |--bfoster-at-mme1.com--| 20, 19 -- Subject: Re: [Microscopy] TEM Help with Imaging of Micelle Solutions 20, 19 -- Cc: Brigitte P Sternberg |--brigittnanoanalytical-at-yahoo.com--| 20, 19 -- In-Reply-To: |--200710302127.l9ULRTd5007840-at-ns.microscopy.com--| 20, 19 -- References: |--200710302127.l9ULRTd5007840-at-ns.microscopy.com--| 20, 19 -- Mime-Version: 1.0 20, 19 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
==============================Original Headers============================== 23, 20 -- From bfostermme-at-sbcglobal.net Tue Oct 30 17:51:33 2007 23, 20 -- Received: from smtp111.sbc.mail.mud.yahoo.com (smtp111.sbc.mail.mud.yahoo.com [68.142.198.210]) 23, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id l9UMpXFs002455 23, 20 -- for {microscopy-at-microscopy.com} ; Tue, 30 Oct 2007 17:51:33 -0500 23, 20 -- Message-Id: {200710302251.l9UMpXFs002455-at-ns.microscopy.com} 23, 20 -- Received: (qmail 76608 invoked from network); 30 Oct 2007 22:51:33 -0000 23, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 23, 20 -- s=s1024; d=sbcglobal.net; 23, 20 -- h=Received:X-YMail-OSG:X-Mailer:Date:To:From:Subject:Mime-Version:Content-Type; 23, 20 -- b=t3e8H2FFFXabG0XNknjSWL29X4BAhv1CDy0krHYRqUMKuxmDjZ7WE4Qp8bPLUTsrjcWUmuKJgOpZL5QeY65GBJ7vV3CbUa96Hp00icnJiazAPke1bFenb3Kdwl8uwrdX51G0kUyl6fd3ThsYmcRC3F/Ej129IYlr6x8oQe3BKbk= ; 23, 20 -- Received: from unknown (HELO barbsd505.sbcglobal.net) (bfostermme-at-sbcglobal.net-at-68.94.39.83 with login) 23, 20 -- by smtp111.sbc.mail.mud.yahoo.com with SMTP; 30 Oct 2007 22:51:32 -0000 23, 20 -- X-YMail-OSG: OUy1L0IVM1ksEMPR9W8a2Krt_XU6WL_GGHoMxkNA__1ypMOHFw955NFWVW5udEW9F4CA6LOnlVsXlND1QEmdO6PHzlerrF.qaGrDEJZeEjYR8iC5XxHW9fAogQJnJT5S1Epki6LmgcWJE7AWLkQ5sjPsHyc4z82NNTwBi7I_gCru 23, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 23, 20 -- Date: Tue, 30 Oct 2007 17:48:54 -0600 23, 20 -- To: microscopy-at-microscopy.com 23, 20 -- From: Barbara Foster {bfostermme-at-sbcglobal.net} 23, 20 -- Subject: EM Help with Imaging of Micelle Solutions 23, 20 -- Mime-Version: 1.0 23, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
Does anyone have suggestions about how to get more accurate EDX quantitative analysis results on biology samples? A friend of mine asked about the pratical procudure, but I just have material science background. Thanks in advance.
Regards, Huisheng JIAO
==============================Original Headers============================== 3, 27 -- From huisheng.jiao-at-gmail.com Wed Oct 31 03:50:29 2007 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.191]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id l9V8oSRj026924 3, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 31 Oct 2007 03:50:28 -0500 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so76137rvb 3, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 31 Oct 2007 01:50:28 -0700 (PDT) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=p8oEm5YToZxrvB3/b06btXmq9sdYtaCLikwFD70qNCQ=; 3, 27 -- b=NUhFO3qDgyHSib1GMFExjO6qJ9FrvsI5y4p2NPx30W5wHvgzG/qvxKGIQi/o5PHhqwnoDXC4AwUqulL0hQYvA44gPkCbi9g81Srm8bi/r/yLqZ77VrrVh+0fh5HnTtWifAfcfmRb/Wo12tyNdkHHr7HnXLw5Wx2ifXiOaPc5zUs= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=beta; 3, 27 -- h=received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- b=FLb3JmgVnxu17E+ujKW6ffGxro2q1+VYds1g2UBZMrBsEepgIzzWnXVQL9fuYQDwA2L3/jbXu3Ss2J2DbY8+xdMzqz2+VyJEXgwUFdA50CtrvjtVSEURO82nsz0MbwO0siqpZwnPgxvVCslMv2uo4coGbal99k8phVRZwnlb7C0= 3, 27 -- Received: by 10.114.149.2 with SMTP id w2mr1678128wad.1193820628179; 3, 27 -- Wed, 31 Oct 2007 01:50:28 -0700 (PDT) 3, 27 -- Received: by 10.114.52.3 with HTTP; Wed, 31 Oct 2007 01:50:28 -0700 (PDT) 3, 27 -- Message-ID: {ed8387f0710310150i4784303l71f61894b85f4d9a-at-mail.gmail.com} 3, 27 -- Date: Wed, 31 Oct 2007 08:50:28 +0000 3, 27 -- From: "Huisheng JIAO" {huisheng.jiao-at-gmail.com} 3, 27 -- To: Microscopy-at-microscopy.com 3, 27 -- Subject: EDX on Biology sample 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
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